Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAE485_RS05180 Genome accession   NZ_CP151686
Coordinates   1014950..1016659 (+) Length   569 a.a.
NCBI ID   WP_215861867.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain QBS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009950..1021659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAE485_RS05155 (AAE485_05070) xth 1010540..1011313 (+) 774 WP_278666306.1 exodeoxyribonuclease III -
  AAE485_RS05160 (AAE485_05075) hemH 1011310..1012332 (+) 1023 WP_233470670.1 ferrochelatase -
  AAE485_RS05165 (AAE485_05080) - 1012384..1012872 (+) 489 WP_064218850.1 peptidylprolyl isomerase -
  AAE485_RS05170 (AAE485_05085) - 1012940..1014103 (+) 1164 WP_409409674.1 anhydro-N-acetylmuramic acid kinase -
  AAE485_RS05175 (AAE485_05090) aroE 1014081..1014980 (+) 900 WP_409409675.1 shikimate dehydrogenase -
  AAE485_RS05180 (AAE485_05095) pilB 1014950..1016659 (+) 1710 WP_215861867.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAE485_RS05185 (AAE485_05100) - 1017114..1018382 (+) 1269 WP_409409676.1 two-component system sensor histidine kinase NtrB -
  AAE485_RS05190 (AAE485_05105) pilR 1018379..1019737 (+) 1359 WP_215861728.1 sigma-54 dependent transcriptional regulator Regulator
  AAE485_RS05195 (AAE485_05110) - 1019734..1020534 (+) 801 WP_064220377.1 prepilin peptidase -
  AAE485_RS05200 (AAE485_05115) coaE 1020535..1021137 (+) 603 WP_215861727.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62896.65 Da        Isoelectric Point: 6.4404

>NTDB_id=979818 AAE485_RS05180 WP_215861867.1 1014950..1016659(+) (pilB) [Acidithiobacillus ferriphilus strain QBS3]
MATNPAHITLTPVLRALVSNGLSDEAQLQRLAADPARGKMPLLFYAVERGAVPASVLMAHLSARYNMPMLDLDAVMINEL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNTGLQVLPILVEANKLVKAVNAAANSMQGGIDDVFMD
KPRGEEEQEEFDLAQESDGSVEDAPVVRFARQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDVLHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFLPDQRKAFEEAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLNFATALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCPHCKKPLRIPEQALIEAGF
THEDLQGWRPLGAVGCDQCNNTGYKGRMGLYQVMPVSDAMREIIMRGGTSIDLARQATQEGVLTMRQNGLRRVREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=979818 AAE485_RS05180 WP_215861867.1 1014950..1016659(+) (pilB) [Acidithiobacillus ferriphilus strain QBS3]
ATGGCTACAAATCCAGCACACATCACGTTGACGCCCGTATTGCGGGCGTTGGTCAGCAACGGGTTAAGCGATGAGGCGCA
GTTGCAACGCCTTGCCGCCGATCCGGCGCGTGGCAAGATGCCGCTGCTGTTTTATGCGGTTGAAAGGGGTGCCGTTCCTG
CCTCTGTGCTTATGGCGCACCTTTCTGCGCGCTATAACATGCCCATGCTTGATCTGGATGCGGTCATGATTAATGAGTTG
CTTATTCGGAAGCTCGACAAAGGACTGATGACCCGCTATCTGGTGCTGCCCTTGTCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCGGACCCTACGGATTTTAAGGCGGTGGAGGATGTGAAGTTCAACACGGGTCTGCAGGTGTTGCCAATACTGG
TCGAGGCCAATAAGCTGGTAAAGGCGGTGAATGCCGCAGCCAACAGTATGCAAGGCGGGATTGACGATGTTTTCATGGAT
AAACCGCGCGGCGAAGAAGAGCAGGAAGAATTCGATCTTGCCCAGGAGAGTGATGGCAGTGTCGAGGATGCACCCGTGGT
GCGTTTTGCCCGGCAATTGCTGTTGGATGCCATTCAACGCGGGGTTTCCGACATTCATCTGGAACCGTACGAAAAGGACT
TTCGGGTGCGTTACCGACTCGATGGCGTATTGCAGGATGTGTTGCATCCGCCGGTGGCGCTGCGTGACGGTGTCACTTCG
CGCCTGAAGATTCTCTGTCGTCTGGATATTTCTGAGCGGCGTTTACCCCAGGATGGCCGTTTGCGGGTCAGGGTGCCGCC
GGCGCGGGTTATTGATTTCCGGGTTTCTTTTTTGCCCACCAGTTTTGGCGAAACCATTGTGCTGCGCTTGCTGGATCCGG
CCAGTTCCAAGGTGCCCATAGAACAACTGGGCTTTTTGCCGGACCAGCGCAAAGCTTTTGAAGAGGCCATCCACCGGCCC
TACGGTATGATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACGACGACTCTTTACACCGCGCTGAATATTCTCAATAC
CGGCGACTGCAATATCAGTACCGCAGAGGATCCTGTGGAAATCCCGGTGTACGGGATTAATCAGGTGAATATCAACGAGC
GTATCGGTCTCAACTTTGCCACCGCCTTGCGTTCCTTTCTGCGTCAGGACCCTGACATCATTATGGTCGGTGAGGTGCGT
GATCTGGAAACCGCGGAAACGGCCATCAAAGCCGCCCAGACGGGTCATCTGGTGTTGGCCACCCTGCATACCAATGATGC
ACCACAGAGTCTCACCCGTCTGGAAAATATGGGCATCCCTACCTATAACATAGCAGGCGGTGTGCACTTGGTGATGGCGC
AGCGACTCGCACGCAAACTCTGCCCGCATTGTAAAAAGCCGCTGCGCATTCCCGAACAGGCCCTGATTGAGGCGGGATTT
ACGCACGAGGATCTGCAGGGTTGGCGTCCTCTGGGCGCGGTGGGCTGTGATCAGTGCAACAACACCGGATATAAAGGCCG
GATGGGACTCTATCAGGTGATGCCGGTGAGCGATGCTATGCGTGAAATCATTATGCGCGGTGGTACCTCCATCGATCTGG
CGCGGCAGGCGACACAAGAGGGAGTGCTGACCATGCGGCAAAACGGATTGAGGCGGGTCAGGGAGGGTGTCACTAGTCTT
GAAGAGGTCCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.354

100

0.49
  pilB Acinetobacter baumannii D1279779

48.063

99.824

0.48
  pilB Acinetobacter baylyi ADP1

46.479

99.824

0.464
  pilF Neisseria gonorrhoeae MS11

45.276

98.594

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

43.106

100

0.434
  pilB Vibrio cholerae strain A1552

46.641

91.564

0.427
  pilB Vibrio campbellii strain DS40M4

44.828

91.74

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.322

0.371

Multiple sequence alignment