Detailed information NTG0097980

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: ACGHT2_RS10545
• Genome accession: NZ_AP031455
• Coordinates: 2018784..2019782 (-)
• Length: 332 a.a.
• NCBI ID: WP_003066863.1
• Uniprot ID: E0PGS8
• Organism: Streptococcus pasteurianus strain k46-0107-A9
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2013784..2024782

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACGHT2_RS10530 (K460107A9_19830)	treP	2014357..2016330 (-)	1974	WP_270619807.1	PTS system trehalose-specific EIIBC component	-
ACGHT2_RS10535 (K460107A9_19840)	treR	2016527..2017240 (+)	714	WP_003066857.1	trehalose operon repressor	Regulator
ACGHT2_RS10540 (K460107A9_19850)	-	2017650..2018000 (-)	351	Protein_2018	isopeptide-forming domain-containing fimbrial protein	-
ACGHT2_RS10545 (K460107A9_19860)	ruvB	2018784..2019782 (-)	999	WP_003066863.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
ACGHT2_RS10550 (K460107A9_19870)	nrdG	2019911..2020525 (-)	615	WP_003066866.1	anaerobic ribonucleoside-triphosphate reductase activating protein	-
ACGHT2_RS10555 (K460107A9_19880)	-	2020566..2021072 (-)	507	WP_003066869.1	GNAT family N-acetyltransferase	-
ACGHT2_RS10560 (K460107A9_19890)	-	2021084..2021230 (-)	147	WP_013852326.1	hypothetical protein	-
ACGHT2_RS10565 (K460107A9_19900)	nrdD	2021471..2023687 (-)	2217	WP_003066874.1	anaerobic ribonucleoside-triphosphate reductase	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37509.92 Da
• Isoelectric Point: 4.5455

>NTDB_id=97980 ACGHT2_RS10545 WP_003066863.1 2018784..2019782(-) (ruvB) [Streptococcus pasteurianus strain k46-0107-A9]
MSRILDNDIMGDEEFAERTLRPQFLKEYIGQDKVKNQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVITNELGVNL
KQTSGPAIEKAGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGNGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYDVEDLTEIVERTADIFEMEIVHEAAQELARRSRGTPRIANRLLKRVRDYAQIMGDGIIT
KDITDKALTMLDVDHEGLDYIDQKILRTMIEVYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATEK
AYEHLGYPYSEK

Nucleotide

• Length: 999 bp

>NTDB_id=97980 ACGHT2_RS10545 WP_003066863.1 2018784..2019782(-) (ruvB) [Streptococcus pasteurianus strain k46-0107-A9]
ATGAGCAGAATTTTAGATAATGATATTATGGGTGATGAGGAATTTGCGGAGCGCACGCTTCGTCCTCAGTTTTTAAAAGA
ATATATCGGACAAGATAAGGTTAAAAATCAGCTAAAAATCTTTATTGAGGCAGCAAAATTACGTGACGAGTCTTTGGACC
ATGTGCTATTGTTTGGACCTCCAGGTCTTGGTAAAACGACAATGGCATTTGTGATTACTAATGAATTAGGGGTGAATTTA
AAACAGACATCTGGACCTGCTATCGAAAAAGCTGGTGATTTGGTTGCTATCTTGAATGATTTGGAACCTGGTGATGTGCT
TTTTATTGACGAAATTCATCGTATGCCAATGGCTGTGGAAGAAGTACTTTATAGCGCCATGGAAGATTTTTATATTGACA
TCATGATTGGCAATGGCGAGACTAGTCGTAGTGTTCACTTGGATTTACCGCCGTTTACCCTGATTGGTGCGACAACACGT
GCTGGTATGCTTTCTAATCCTTTACGTGCTCGTTTTGGCATTACTGGGCATATGGAATACTATGACGTTGAAGATTTGAC
AGAAATTGTTGAACGCACGGCAGATATTTTTGAAATGGAAATTGTCCATGAAGCTGCCCAAGAATTGGCACGACGTAGTC
GTGGAACACCTCGTATTGCTAATCGTCTCTTAAAACGTGTACGTGATTACGCCCAAATTATGGGAGATGGTATTATCACA
AAAGATATTACCGATAAAGCATTGACGATGTTAGATGTTGACCATGAGGGGCTTGATTACATTGACCAGAAAATTTTGCG
TACCATGATTGAGGTTTATAACGGTGGTCCTGTCGGTTTAGGAACGCTATCCGTTAATATCGCTGAAGAGCGTGATACTG
TTGAGGATATGTATGAGCCGTATTTGATTCAAAAAGGCTTTATTATGCGCACTCGTACTGGTCGTGTGGCAACTGAAAAA
GCTTATGAACATTTGGGCTATCCTTATTCGGAAAAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

3D structure

• AlphaFold DB: E0PGS8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	89.157	100	0.892
ruvB	Streptococcus pneumoniae R6	89.157	100	0.892
ruvB	Streptococcus pneumoniae D39	89.157	100	0.892
ruvB	Bacillus subtilis subsp. subtilis str. 168	61.61	97.289	0.599
ruvB	Helicobacter pylori 26695	49.693	98.193	0.488
ruvB	Synechocystis sp. PCC 6803	49.521	94.277	0.467