Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACNHKQ_RS21080 Genome accession   NZ_CP182139
Coordinates   4283175..4284413 (-) Length   412 a.a.
NCBI ID   WP_000815972.1    Uniprot ID   -
Organism   Escherichia coli strain CUWW06     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4278175..4289413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNHKQ_RS21060 (ACNHKQ_21060) dam 4278787..4279623 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  ACNHKQ_RS21065 (ACNHKQ_21065) damX 4279730..4281016 (-) 1287 WP_000343210.1 cell division protein DamX -
  ACNHKQ_RS21070 (ACNHKQ_21070) aroB 4281108..4282196 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  ACNHKQ_RS21075 (ACNHKQ_21075) aroK 4282253..4282774 (-) 522 WP_000818618.1 shikimate kinase AroK -
  ACNHKQ_RS21080 (ACNHKQ_21080) comE 4283175..4284413 (-) 1239 WP_000815972.1 DNA uptake porin HofQ Machinery gene
  ACNHKQ_RS21085 (ACNHKQ_21085) hofP 4284325..4284729 (-) 405 WP_001264141.1 DNA utilization protein HofP -
  ACNHKQ_RS21090 (ACNHKQ_21090) hofO 4284719..4285159 (-) 441 WP_032281706.1 DNA utilization protein HofO -
  ACNHKQ_RS21095 (ACNHKQ_21095) hofN 4285143..4285682 (-) 540 WP_032281707.1 DNA utilization protein HofN -
  ACNHKQ_RS21100 (ACNHKQ_21100) hofM 4285682..4286461 (-) 780 WP_032281708.1 DNA utilization protein HofM -
  ACNHKQ_RS21105 (ACNHKQ_21105) mrcA 4286581..4289133 (+) 2553 WP_032281709.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44735.24 Da        Isoelectric Point: 5.9488

>NTDB_id=979543 ACNHKQ_RS21080 WP_000815972.1 4283175..4284413(-) (comE) [Escherichia coli strain CUWW06]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=979543 ACNHKQ_RS21080 WP_000815972.1 4283175..4284413(-) (comE) [Escherichia coli strain CUWW06]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAAAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCCCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAAGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCAGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362