Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAEO81_RS28605 Genome accession   NZ_CP151650
Coordinates   6381473..6382537 (-) Length   354 a.a.
NCBI ID   WP_166596131.1    Uniprot ID   -
Organism   Pseudomonas sp. RC10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6376473..6387537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEO81_RS28580 (AAEO81_28580) aroK 6377051..6377569 (-) 519 WP_110950932.1 shikimate kinase AroK -
  AAEO81_RS28585 (AAEO81_28585) pilQ 6377574..6379664 (-) 2091 WP_166596127.1 type IV pilus secretin PilQ Machinery gene
  AAEO81_RS28590 (AAEO81_28590) pilP 6379718..6380245 (-) 528 WP_166596128.1 type 4a pilus biogenesis lipoprotein PilP -
  AAEO81_RS28595 (AAEO81_28595) pilO 6380242..6380889 (-) 648 WP_166596129.1 type 4a pilus biogenesis protein PilO -
  AAEO81_RS28600 (AAEO81_28600) - 6380886..6381473 (-) 588 WP_166596130.1 PilN domain-containing protein -
  AAEO81_RS28605 (AAEO81_28605) pilM 6381473..6382537 (-) 1065 WP_166596131.1 pilus assembly protein PilM Machinery gene
  AAEO81_RS28610 (AAEO81_28610) - 6382744..6385221 (+) 2478 WP_341960479.1 penicillin-binding protein 1A -
  AAEO81_RS28615 (AAEO81_28615) - 6385408..6386676 (-) 1269 WP_341960480.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38218.78 Da        Isoelectric Point: 4.6320

>NTDB_id=979529 AAEO81_RS28605 WP_166596131.1 6381473..6382537(-) (pilM) [Pseudomonas sp. RC10]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGNALTRVLVKAKTNVKIAAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVDVLLAACRKENVEVREAALAL
ANLTARVVDVEAYALERSFGLLSAQLGQDHEQLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSL
GEAGLAKKQGGLPDDYTLEVLQPFKDAVVQQVSRSLQFFFAAGQYNRVDYIMLAGGTASIAGLDRLIQERLGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=979529 AAEO81_RS28605 WP_166596131.1 6381473..6382537(-) (pilM) [Pseudomonas sp. RC10]
GTGTTCGAACTCTTCAGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTGAAACTCCTTGAACT
GAGTCGTTCCGGCACCCGCTACAAGGTCGAGTCTTACGCGGTCGAGCCACTGCCCGCCAACGCGGTGGTGGAAAAAAACA
TTGCCGAACTGGAAGGGGTCGGCAATGCGTTGACGCGGGTGTTGGTAAAAGCCAAGACAAACGTCAAAATCGCGGCGGTT
GCAGTGGCGGGTTCGGCGGTCATTACCAAGACCATCGAGATGGATGCCGGTCTTTCCGACGACGAGATGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCGCTTGAAGAAGTTGCTATCGATTTCGAGGTGCAAGGCTATTCGGCAC
GCAATCCCGAGCGTGTCGATGTGCTGCTCGCTGCCTGCCGCAAGGAAAACGTCGAAGTCCGCGAAGCTGCGTTGGCGCTG
GCCAACCTGACCGCGCGCGTGGTCGATGTTGAAGCCTACGCGTTGGAGCGCTCCTTCGGCCTGTTGTCCGCTCAGTTGGG
TCAGGATCACGAACAACTGACCGTCGCAGTCATCGACATCGGCGCCACCATGACCACACTCAGCGTGCTGCACAACGGCC
GCATCATTTATACCCGCGAGCAACTGTTTGGCGGGCGTCAGCTCACTGAAGAAATTCAGCGCCGTTACGGCCTGTCCCTC
GGCGAAGCCGGGCTTGCGAAGAAGCAGGGCGGCTTGCCGGACGACTACACGCTAGAAGTGCTGCAGCCGTTCAAGGACGC
CGTGGTTCAGCAGGTTTCCCGCTCGTTGCAGTTCTTCTTCGCCGCCGGTCAGTACAACCGTGTCGACTACATCATGCTGG
CAGGCGGGACGGCATCCATTGCCGGACTGGACCGTCTGATCCAGGAACGCCTGGGCACCCCGACCATGGTCGCCAACCCT
TTTGCCGACATGGCGCTCAGCGCCAAGGTGAACGCGGGCGCGCTGGCGAGTGACGCCCCGGCCTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment