Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAEO81_RS28585 Genome accession   NZ_CP151650
Coordinates   6377574..6379664 (-) Length   696 a.a.
NCBI ID   WP_166596127.1    Uniprot ID   -
Organism   Pseudomonas sp. RC10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6372574..6384664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEO81_RS28570 (AAEO81_28570) - 6374172..6375806 (-) 1635 WP_341960473.1 AAA family ATPase -
  AAEO81_RS28575 (AAEO81_28575) aroB 6375817..6376920 (-) 1104 WP_166596126.1 3-dehydroquinate synthase -
  AAEO81_RS28580 (AAEO81_28580) aroK 6377051..6377569 (-) 519 WP_110950932.1 shikimate kinase AroK -
  AAEO81_RS28585 (AAEO81_28585) pilQ 6377574..6379664 (-) 2091 WP_166596127.1 type IV pilus secretin PilQ Machinery gene
  AAEO81_RS28590 (AAEO81_28590) pilP 6379718..6380245 (-) 528 WP_166596128.1 type 4a pilus biogenesis lipoprotein PilP -
  AAEO81_RS28595 (AAEO81_28595) pilO 6380242..6380889 (-) 648 WP_166596129.1 type 4a pilus biogenesis protein PilO -
  AAEO81_RS28600 (AAEO81_28600) - 6380886..6381473 (-) 588 WP_166596130.1 PilN domain-containing protein -
  AAEO81_RS28605 (AAEO81_28605) pilM 6381473..6382537 (-) 1065 WP_166596131.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 74760.05 Da        Isoelectric Point: 5.4069

>NTDB_id=979528 AAEO81_RS28585 WP_166596127.1 6377574..6379664(-) (pilQ) [Pseudomonas sp. RC10]
MTRILSIIGVSLWMAMLSPILQAANLKTLDVAALPGDRVELKLSFDGPVAAPRGYTTEQPARIALDLPGVTSQLAIKSRD
LGSGNAHSVVVVEAKDRTRVIINLTTLAPYSSRVDGNNLFVVVGQGAAAAQGSQPSTAQGAPRVSVPPATASAAQAKPAP
RSFAPGPRSIKNVDFQRGELGEGNVVIELSDAGIAPDIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVNSSVSG
DKASISIEPSGAFDYSAYQTENKLTISVRPLTNEDVARRNADKLVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDT
VQGGITLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIA
KLFQSVTSAESKTDERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSTT
GGKYTTGGNGTVTGAGNTNGPYVDMGVANPTSSIGLGFLTNNTILDLELTAMEKTGNGEVVSQPKVVTSDKETAKILKGT
EVPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVADGETIVIGG
VFSNTQNKVVDKVPFLGDVPYVGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=979528 AAEO81_RS28585 WP_166596127.1 6377574..6379664(-) (pilQ) [Pseudomonas sp. RC10]
ATGACTAGGATTCTTTCGATTATCGGCGTGTCGCTATGGATGGCGATGCTTTCACCGATTCTCCAGGCGGCCAACCTCAA
GACGCTCGATGTCGCCGCATTGCCGGGGGATCGGGTCGAGTTGAAACTTTCCTTCGACGGCCCTGTGGCCGCCCCGCGCG
GCTATACCACCGAGCAACCGGCGCGCATCGCGCTGGACTTGCCCGGCGTGACCAGCCAACTGGCGATCAAGAGCCGGGAC
CTGGGGTCGGGTAACGCTCACAGTGTCGTGGTGGTCGAAGCCAAGGATCGCACCCGTGTGATCATCAACCTGACCACGCT
CGCGCCCTATAGCTCCCGCGTGGATGGCAACAACCTGTTCGTCGTCGTCGGGCAGGGTGCCGCCGCCGCGCAAGGTTCAC
AGCCAAGTACCGCCCAAGGCGCGCCGCGCGTGAGCGTGCCTCCTGCAACGGCCAGCGCCGCGCAAGCCAAACCGGCCCCT
CGCTCCTTTGCGCCGGGTCCGCGTTCGATCAAGAACGTCGATTTCCAGCGCGGCGAACTGGGTGAAGGCAACGTTGTGAT
CGAGCTGAGCGATGCAGGCATTGCGCCGGACATTCAGGAGCAGGGCGGCAAGATTCGTGTGGATTTCGCCAAGACTCAAC
TGCCTGATCCGTTGCGCGTACGCCTCGACGTGAAGGATTTCGCCACACCGGTCCAGTTCGTGAACTCCAGCGTGAGCGGC
GACAAGGCGAGCATCTCCATCGAGCCGTCCGGTGCGTTCGATTACTCGGCCTATCAAACCGAAAACAAACTGACCATCAG
CGTGCGTCCGTTGACCAACGAAGACGTCGCTCGCCGCAATGCCGACAAGCTGGTCTACACCGGCGAGAAACTGTCGCTGA
ACTTCCAGGACATTGACGTGCGCTCGGTGCTGCAATTGATCGCCGACTTCACCAATCTCAATCTGGTGGCCAGTGACACC
GTTCAAGGCGGCATCACCCTGCGTCTGCAAAACGTGCCGTGGGACCAGGCGCTTGACCTGGTGCTCAAGACCAAAGGCCT
GGACAAGCGCAAGATCGGCAACGTGTTGCTGGTCGCGCCTGCTGACGAGATTGCCGCTCGTGAACGTCAGGAGCTGGAAT
CGCTGAAGCAGATTGCCGAGCTCGCACCGCTGCGTCGTGAACTGCTGCAAGTGAACTATGCCAAGGCTGCGGACATCGCC
AAGCTGTTCCAGTCGGTGACCAGCGCTGAGTCGAAGACCGACGAGCGCGGTTCGATCACCGTCGATGAGCGGACCAACAA
CATCATCGCCTATCAAACGCAGGACCGGCTCGATGAGCTGCGCCGTATCGTCTCGCAGCTGGACATTCCTGTGCGTCAGG
TGATGATCGAGGCGCGCATCGTCGAAGCCAACGTCGACTACGACAAGGCGCTGGGTGTGCGTTGGGGCGGTTCCACCACC
GGCGGTAAATACACCACGGGCGGTAATGGCACGGTGACGGGCGCTGGTAACACCAATGGCCCATACGTGGACATGGGAGT
GGCCAACCCAACGTCTTCGATTGGTCTGGGCTTCCTGACCAACAACACGATTCTCGACCTTGAACTGACCGCCATGGAAA
AAACCGGTAACGGTGAGGTGGTGTCGCAACCCAAGGTCGTCACTTCGGACAAGGAAACCGCGAAAATTCTGAAAGGTACA
GAAGTGCCGTATCAGGAAGCGAGTTCCAGCGGTGCGACGTCGGTGTCGTTCAAAGAGGCGTCGTTGTCCCTGGAAGTAAC
CCCGCAGATCACTCCGGACAACCGCATCATCATGGAAGTGAAGGTGACCAAGGACGAACCTGACTACGTGAACACCGTGT
TGGGCGTACCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTGGCCGACGGCGAAACCATCGTGATTGGTGGC
GTGTTCTCCAATACACAGAATAAAGTCGTCGATAAGGTGCCATTTTTGGGCGATGTGCCGTATGTTGGCCGCCTTTTCCG
GCGGGATGTTGTCTCGGAGAAAAAATCCGAGCTGTTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(314-360)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.299

100

0.75
  comQ Acinetobacter baylyi ADP1

42.489

100

0.427
  pilQ Acinetobacter baumannii strain A118

39.154

100

0.412
  pilQ Acinetobacter baumannii D1279779

39.154

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

39.447

98.707

0.389
  pilQ Legionella pneumophila strain Lp02

39.301

98.707

0.388

Multiple sequence alignment