Detailed information NTG0097828

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: R8619_RS09745
• Genome accession: NZ_AP026918
• Coordinates: 1874538..1874999 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250540.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain PZ900700009
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1869538..1879999

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R8619_RS09710 (PC0009_18800)	-	1869842..1870666 (-)	825	WP_001832473.1	membrane protein insertase YidC	-
R8619_RS09715 (PC0009_18810)	rnpA	1870641..1871012 (-)	372	WP_000739244.1	ribonuclease P protein component	-
R8619_RS09720	-	1871029..1871160 (-)	132	WP_000768904.1	hypothetical protein	-
R8619_RS09725 (PC0009_18820)	-	1871161..1872351 (-)	1191	WP_000167752.1	acetate kinase	-
R8619_RS09730 (PC0009_18830)	comYH	1872402..1873355 (-)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
R8619_RS09735	-	1873416..1874010 (-)	595	Protein_1890	class I SAM-dependent methyltransferase	-
R8619_RS09740 (PC0009_18860)	comGG/cglG	1874147..1874560 (-)	414	WP_000265620.1	competence type IV pilus minor pilin ComGG	Machinery gene
R8619_RS09745 (PC0009_18870)	comGF/cglF	1874538..1874999 (-)	462	WP_000250540.1	competence type IV pilus minor pilin ComGF	Machinery gene
R8619_RS09750 (PC0009_18880)	comGE/cglE	1874962..1875264 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
R8619_RS09755 (PC0009_18890)	comGD/cglD	1875227..1875631 (-)	405	WP_000588023.1	competence type IV pilus minor pilin ComGD	Machinery gene
R8619_RS09760 (PC0009_18900)	comGC/cglC	1875624..1875950 (-)	327	WP_050260822.1	comG operon protein ComGC	Machinery gene
R8619_RS09765 (PC0009_18910)	comGB/cglB	1875952..1876968 (-)	1017	WP_073177425.1	competence type IV pilus assembly protein ComGB	Machinery gene
R8619_RS09770 (PC0009_18920)	comGA/cglA/cilD	1876916..1877857 (-)	942	WP_000249559.1	competence type IV pilus ATPase ComGA	Machinery gene
R8619_RS09775 (PC0009_18930)	-	1877933..1878298 (-)	366	WP_000286415.1	DUF1033 family protein	-
R8619_RS09780 (PC0009_18940)	-	1878565..1879820 (+)	1256	WP_370657740.1	ISL3 family transposase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17850.40 Da
• Isoelectric Point: 8.0016

>NTDB_id=97828 R8619_RS09745 WP_000250540.1 1874538..1874999(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700009]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=97828 R8619_RS09745 WP_000250540.1 1874538..1874999(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700009]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	92.157	100	0.922