Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   R8563_RS09800 Genome accession   NZ_AP026917
Coordinates   1878277..1878738 (-) Length   153 a.a.
NCBI ID   WP_000250523.1    Uniprot ID   A0A0B7M7T3
Organism   Streptococcus pneumoniae strain PZ900700007     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1873277..1883738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8563_RS09765 (PC0007_18900) - 1873582..1874406 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  R8563_RS09770 (PC0007_18910) rnpA 1874381..1874752 (-) 372 WP_000739246.1 ribonuclease P protein component -
  R8563_RS09775 - 1874769..1874900 (-) 132 WP_000768904.1 hypothetical protein -
  R8563_RS09780 (PC0007_18920) - 1874901..1876091 (-) 1191 WP_000167768.1 acetate kinase -
  R8563_RS09785 (PC0007_18930) comYH 1876142..1877095 (-) 954 WP_000345086.1 class I SAM-dependent methyltransferase Machinery gene
  R8563_RS09790 - 1877156..1877750 (-) 595 Protein_1901 class I SAM-dependent methyltransferase -
  R8563_RS09795 (PC0007_18960) comGG/cglG 1877853..1878299 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8563_RS09800 (PC0007_18970) comGF/cglF 1878277..1878738 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8563_RS09805 (PC0007_18980) comGE/cglE 1878701..1879003 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8563_RS09810 (PC0007_18990) comGD/cglD 1878966..1879370 (-) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8563_RS09815 (PC0007_19000) comGC/cglC 1879363..1879689 (-) 327 WP_000738621.1 comG operon protein ComGC Machinery gene
  R8563_RS09820 (PC0007_19010) comGB/cglB 1879691..1880707 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8563_RS09825 (PC0007_19020) comGA/cglA/cilD 1880655..1881596 (-) 942 WP_000249568.1 competence type IV pilus ATPase ComGA Machinery gene
  R8563_RS09830 (PC0007_19030) - 1881672..1882037 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8563_RS09835 (PC0007_19040) - 1882188..1883246 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=97749 R8563_RS09800 WP_000250523.1 1878277..1878738(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700007]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=97749 R8563_RS09800 WP_000250523.1 1878277..1878738(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700007]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGAT
TGTCATCAGTGGGGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M7T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

92.157

100

0.922

Multiple sequence alignment