Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AABM54_RS02110 Genome accession   NZ_CP150918
Coordinates   468237..469301 (+) Length   354 a.a.
NCBI ID   WP_347903403.1    Uniprot ID   -
Organism   Pseudomonas purpurea strain DGS26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463237..474301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM54_RS02100 - 464100..465368 (+) 1269 WP_347903399.1 malic enzyme-like NAD(P)-binding protein -
  AABM54_RS02105 - 465581..468022 (-) 2442 WP_347903401.1 penicillin-binding protein 1A -
  AABM54_RS02110 pilM 468237..469301 (+) 1065 WP_347903403.1 pilus assembly protein PilM Machinery gene
  AABM54_RS02115 - 469301..469867 (+) 567 WP_347903405.1 PilN domain-containing protein -
  AABM54_RS02120 pilO 469864..470487 (+) 624 WP_347903407.1 type 4a pilus biogenesis protein PilO -
  AABM54_RS02125 - 470484..471011 (+) 528 WP_347903409.1 pilus assembly protein PilP -
  AABM54_RS02130 pilQ 471025..473115 (+) 2091 WP_347903410.1 type IV pilus secretin PilQ Machinery gene
  AABM54_RS02135 aroK 473120..473638 (+) 519 WP_347903411.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38107.72 Da        Isoelectric Point: 4.8170

>NTDB_id=973770 AABM54_RS02110 WP_347903403.1 468237..469301(+) (pilM) [Pseudomonas purpurea strain DGS26]
MLGLFNKKNRTLLGIDISSTSVKLLELSRSGTRYRVEAYAVEPLPANAVVEKNIAELEGVGQALTRVLLKARSSVRTVAV
AVAGSAVITKTLEMDAGLSADELENQLKIEADQYIPYPLEEVAIDFEVQGPSLRNPERIDVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLASSQERLTVAVVDIGATMTTLSVLLNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNTVDHILLAGGTASIAGLDRLIEQRLGTPTQVANP
FSDMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=973770 AABM54_RS02110 WP_347903403.1 468237..469301(+) (pilM) [Pseudomonas purpurea strain DGS26]
GTGCTGGGACTCTTCAATAAAAAAAACCGGACACTTCTGGGGATCGACATCAGCTCGACGTCGGTGAAGCTCCTGGAGCT
GAGCCGCTCAGGCACCCGTTACCGGGTCGAGGCCTACGCCGTAGAGCCGTTGCCGGCCAACGCGGTCGTGGAAAAAAACA
TCGCTGAACTCGAAGGGGTAGGCCAGGCGCTCACGCGTGTCCTGCTCAAGGCCAGGAGCAGCGTGCGCACGGTTGCCGTC
GCGGTGGCCGGGTCTGCGGTGATCACCAAGACCCTTGAGATGGATGCAGGGCTCTCTGCCGACGAACTCGAAAACCAGCT
GAAGATCGAAGCCGATCAGTACATTCCCTATCCACTGGAAGAAGTCGCCATCGATTTCGAAGTCCAGGGCCCGTCCCTGC
GTAACCCCGAACGCATCGACGTGCTGCTGGCCGCATGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTG
GCCGGGCTGACCGCACGGGTGGTCGATGTCGAGGCCTACGCACTGGAGCGCTCTTTCGGGTTGCTGGCGACTCAACTGGC
CAGTTCCCAGGAGCGCCTGACGGTAGCGGTGGTCGACATCGGTGCCACCATGACCACCCTGAGCGTGCTGCTCAACGGCA
AGATCATCTACACCCGCGAGCAGCTCTTTGGCGGTCGCCAGCTCACCGAAGAGATCCAGCGTCGTTATGGCCTGACCGTC
GAACAGGCCGGGCTGGCAAAAAAACAGGGCGGCCTGCCCGACGATTACGTCAGCGAAGTCCTGCAACCTTTCAGGGAAGC
GCTGGTGCAGCAGGTGTCCCGATCCCTTCAGTTCTTTTTCGCTTCCGGCCAGTACAACACGGTCGACCACATCCTGTTGG
CGGGGGGGACGGCCTCGATTGCCGGGCTGGACCGGCTGATCGAGCAGCGCCTCGGCACGCCGACCCAGGTAGCCAACCCT
TTCTCGGACATGGCCCTGAGCGCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCACCAGCGCTGATGATTGCCTGCGG
CCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment