Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WOB24_RS16085 Genome accession   NZ_CP150891
Coordinates   3441375..3442439 (-) Length   354 a.a.
NCBI ID   WP_339080306.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3436375..3447439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS16060 (WOB24_16060) aroK 3437005..3437523 (-) 519 WP_339080303.1 shikimate kinase AroK -
  WOB24_RS16065 (WOB24_16065) pilQ 3437528..3439645 (-) 2118 WP_341303928.1 type IV pilus secretin PilQ Machinery gene
  WOB24_RS16070 (WOB24_16070) pilP 3439662..3440186 (-) 525 WP_341303929.1 type 4a pilus biogenesis lipoprotein PilP -
  WOB24_RS16075 (WOB24_16075) pilO 3440183..3440806 (-) 624 WP_341303930.1 type 4a pilus biogenesis protein PilO -
  WOB24_RS16080 (WOB24_16080) pilN 3440803..3441375 (-) 573 WP_341303931.1 type 4a pilus biogenesis protein PilN -
  WOB24_RS16085 (WOB24_16085) pilM 3441375..3442439 (-) 1065 WP_339080306.1 pilus assembly protein PilM Machinery gene
  WOB24_RS16090 (WOB24_16090) - 3442621..3445038 (+) 2418 WP_341303932.1 penicillin-binding protein 1A -
  WOB24_RS16095 (WOB24_16095) - 3445135..3446403 (-) 1269 WP_339080308.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37825.21 Da        Isoelectric Point: 4.4692

>NTDB_id=973536 WOB24_RS16085 WP_339080306.1 3441375..3442439(-) (pilM) [Pseudomonas sp. TMP25]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLAKSKSGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGSSARNAERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAEQLGGGRDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVARSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FAEMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=973536 WOB24_RS16085 WP_339080306.1 3441375..3442439(-) (pilM) [Pseudomonas sp. TMP25]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTACTCGGGATCGATATCAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGCCGCTCCGGAAGCCGCTACAAGGTAGAGGCTTACGCAGTCGAGCCGCTCCCGCCAAACGCGGTGGTCGAAAAAAACA
TTGCCGAATTGGAGGGTGTTGGCCAAGCGCTTGCGCGCGTGCTCGCCAAATCCAAGAGCGGTGTAAAAACCGTGGCGGTG
GCGGTGGCCGGTTCAGCAGTGATTACCAAAACCATTGAGATGGAAGCAGGGCTTTCTGACGATGAGTTGGAAAATCAGCT
GAAGATCGAAGCTGACCAATACATCCCCTATCCGCTGGAAGAAGTGGCAATCGACTTTGAAGTGCAGGGCTCATCCGCCC
GCAACGCCGAGCGCGTTGAAGTACTACTCGCCGCTTGCCGCAAGGAAAACGTCGAGGTTCGCGAAGCCGCTTTGGCGCTT
GCTGGCCTGACTGCAAAAGTGGTTGATGTTGAGGCGTATGCCCTTGAGCGTGCTTACGGTCTGCTAGCCGAGCAGTTGGG
TGGTGGTCGCGATGAGCTGACTGTTGCGGTTGTAGATATCGGCGCAACCATGACCACCCTCAGCGTGCTGCACAACGGTC
GCACCATCTATACGCGCGAGCAGTTGTTTGGCGGCAAGCAGCTAACGGAAGAAATTCAACGGCGTTATGGTTTATCCGTT
GAGGAGGCCGGTCTTGCTAAGAAGCAAGGCGGTTTACCGGATGACTATGACAGCGAAGTGCTACAGCCTTTTAAAGAGGC
GGTGGTGCAGCAGGTAGCTCGCTCCCTGCAATTTTTCTTCGCCGCCGGCCAATTCAATGACGTCGACTACATCCTGTTAG
CAGGCGGTACTGCGTCAATCCCAGATCTAGATCGTCTGATCCAACAGAAAATAGGCACCCAGACATTGGTCGCCAATCCA
TTCGCTGAAATGGCACTGAGTAGCAAGGTCAATGCAGGCGCGCTTGCCAGTGATGCGCCAGCCTTGATGATTGCTTGTGG
TCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment