Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   WOB24_RS16065 Genome accession   NZ_CP150891
Coordinates   3437528..3439645 (-) Length   705 a.a.
NCBI ID   WP_341303928.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3432528..3444645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS16050 (WOB24_16050) - 3434262..3435839 (-) 1578 WP_341303926.1 AAA family ATPase -
  WOB24_RS16055 (WOB24_16055) aroB 3435850..3436953 (-) 1104 WP_341303927.1 3-dehydroquinate synthase -
  WOB24_RS16060 (WOB24_16060) aroK 3437005..3437523 (-) 519 WP_339080303.1 shikimate kinase AroK -
  WOB24_RS16065 (WOB24_16065) pilQ 3437528..3439645 (-) 2118 WP_341303928.1 type IV pilus secretin PilQ Machinery gene
  WOB24_RS16070 (WOB24_16070) pilP 3439662..3440186 (-) 525 WP_341303929.1 type 4a pilus biogenesis lipoprotein PilP -
  WOB24_RS16075 (WOB24_16075) pilO 3440183..3440806 (-) 624 WP_341303930.1 type 4a pilus biogenesis protein PilO -
  WOB24_RS16080 (WOB24_16080) pilN 3440803..3441375 (-) 573 WP_341303931.1 type 4a pilus biogenesis protein PilN -
  WOB24_RS16085 (WOB24_16085) pilM 3441375..3442439 (-) 1065 WP_339080306.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75523.67 Da        Isoelectric Point: 4.8200

>NTDB_id=973535 WOB24_RS16065 WP_341303928.1 3437528..3439645(-) (pilQ) [Pseudomonas sp. TMP25]
MKKNNQSAQRNVVTNILPSFLGLSLLAALVSPSLMAADLQTLDVSALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDL
PGVTNKLGAKTRELGVGNARSVTVVEAKDRTRLIVNLTSLSPYNTRSEGNSIFIVVGEGVGNSVASNSPAPAPVPVAAKK
YVPQANAISNIDFQRGELGEGNIVITLSDASISPDIQDQGGKIRLDFAGTQLPESLRVKLDVKDFATPVQFVSATGTASK
ASILIEPTGVYDYLAYQTDNKLTISVKPLTQDEAEQRKADSFSYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVA
GNITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESKKQVADLAPLRRELIQVNYAKASDMAQL
FQSVTSADGAVADRGSVTVDDRTNSIIAYQTQERLDELRRIIAQLDVAVRQVMIEARIVEANVDYDKALGVNWRGAGVGD
NNFVVGGSGGDRPGAPGFGQNPGAQNPTPSLGSFVDMGVAGGSGIGIGFITDNTILDLELTAMEKTGNGEIISQPKVVTS
DKETAKILKGSEIPYQEASSSGASTTSFKEASLSLEVTPQITPDNRIIMEVKVTKDSIGQVFPDGVSINKNEVNAKVLVH
DGETIVLGGVFSNTQVKGVDKIPFLGDLPYLGRLFRRDTVKDNKSELLIFLTPRIMNNQAVAVSR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=973535 WOB24_RS16065 WP_341303928.1 3437528..3439645(-) (pilQ) [Pseudomonas sp. TMP25]
ATGAAAAAAAATAACCAATCTGCTCAGCGAAATGTGGTCACGAACATATTGCCCTCTTTTTTAGGCCTTTCCCTTTTAGC
GGCACTCGTCTCGCCATCCCTTATGGCCGCAGACCTGCAAACGCTTGATGTCTCTGCGTTACCGGGAGATCGAGTTGAGT
TGAAGCTCACATTCGATGAGCCTGTTCTTGCGCCTCGCGGTTATACAATCGAGCAGCCCGCGCGTATCGCCCTTGACCTA
CCGGGCGTTACCAACAAGTTGGGTGCCAAGACCAGAGAATTAGGTGTAGGCAATGCACGAAGCGTCACGGTTGTTGAAGC
GAAAGACCGGACGCGTCTTATTGTGAACCTGACTAGCTTATCGCCTTACAACACGCGCTCGGAAGGTAATAGCATTTTTA
TAGTGGTGGGTGAGGGTGTCGGTAATTCAGTCGCCAGTAACTCGCCGGCACCTGCTCCAGTTCCTGTGGCGGCAAAAAAA
TATGTACCTCAAGCCAATGCGATAAGTAATATTGATTTTCAGCGTGGCGAGTTAGGCGAAGGTAATATTGTAATTACCCT
ATCCGATGCCTCGATTAGTCCTGACATACAAGATCAAGGTGGTAAAATTCGCTTGGACTTTGCAGGGACGCAATTGCCTG
AGTCGCTAAGGGTAAAACTGGATGTTAAGGATTTTGCTACGCCTGTTCAGTTTGTCAGTGCGACAGGTACTGCTAGCAAA
GCCAGCATCCTTATCGAGCCTACCGGCGTCTATGATTATTTGGCTTACCAGACCGATAACAAGCTTACTATTAGCGTTAA
GCCGCTAACTCAAGACGAAGCGGAGCAGCGCAAGGCTGATAGCTTCTCCTACACCGGTGAAAAGTTATCTCTCAATTTCC
AAGATATTGATGTTCGCTCTGTTTTGCAGTTAATTGCAGACTTTACTGATCTGAATCTGGTTGCCAGCGATACGGTTGCT
GGGAACATCACGCTGCGCTTGCAAAACGTGCCATGGGATCAGGCTCTTGATCTTGTGCTCAAGACTAAAGGTTTAGATAA
GCGGAAAGTCGGTAATGTGTTGCTGGTTGCGCCAGCTGATGAGATAGCTGCACGTGAGCGTCAAGAGCTTGAGTCCAAGA
AGCAAGTTGCAGACCTTGCGCCTTTGCGCCGCGAGCTTATACAAGTTAATTACGCTAAAGCCTCCGACATGGCCCAGTTA
TTCCAGTCGGTGACCAGTGCCGATGGTGCTGTGGCTGATCGTGGTTCGGTAACTGTTGATGATCGTACTAACAGTATTAT
TGCCTACCAAACCCAAGAGCGACTTGATGAACTGCGTCGTATAATTGCGCAGCTTGATGTGGCCGTGCGCCAGGTGATGA
TTGAGGCTCGGATCGTTGAGGCCAATGTCGATTATGATAAGGCGCTAGGTGTTAACTGGCGCGGTGCCGGGGTTGGCGAT
AATAATTTCGTAGTAGGCGGGAGTGGCGGAGATCGTCCTGGCGCACCCGGTTTCGGACAAAACCCCGGAGCTCAGAATCC
AACTCCATCTCTAGGGTCTTTTGTTGATATGGGCGTAGCCGGGGGGTCTGGAATAGGCATCGGCTTTATAACGGATAACA
CAATCCTTGATCTTGAATTGACGGCTATGGAAAAAACTGGCAATGGCGAGATTATTTCTCAACCAAAAGTAGTTACTTCA
GATAAAGAAACTGCAAAAATACTTAAGGGCTCTGAGATTCCATATCAAGAGGCAAGTTCTAGTGGTGCGAGCACCACATC
TTTTAAAGAAGCCTCACTCTCACTTGAAGTGACTCCGCAAATCACGCCAGATAATAGAATTATTATGGAGGTTAAGGTCA
CCAAGGATTCTATCGGTCAAGTATTCCCAGATGGAGTATCAATTAATAAAAACGAAGTCAACGCTAAGGTTCTAGTTCAT
GATGGAGAAACAATTGTGCTTGGGGGGGTCTTTTCAAATACACAAGTCAAGGGCGTAGATAAGATACCGTTCCTTGGAGA
TCTACCCTATTTGGGTCGATTATTCCGGCGTGATACAGTTAAGGACAATAAGTCAGAACTACTTATCTTCCTTACCCCCC
GCATCATGAATAATCAAGCAGTTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(386-451)

AMIN domain (AMIN)

(52-134)

Bacterial type II and III secretion system protein (Secretin)

(542-696)

Secretin and TonB N terminus short domain (STN)

(312-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.072

100

0.739
  comQ Acinetobacter baylyi ADP1

42.671

97.73

0.417
  pilQ Acinetobacter baumannii D1279779

41.787

98.44

0.411
  pilQ Acinetobacter baumannii strain A118

41.787

98.44

0.411

Multiple sequence alignment