Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AACK07_RS02615 Genome accession   NZ_AP029012
Coordinates   563406..565544 (+) Length   712 a.a.
NCBI ID   WP_275541829.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain MAFF 301069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558406..570544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACK07_RS02595 (MAFF301069_05060) - 560483..561553 (+) 1071 WP_011002880.1 type IV pilus biogenesis protein PilM -
  AACK07_RS02600 (MAFF301069_05070) - 561567..562199 (+) 633 WP_011002879.1 PilN domain-containing protein -
  AACK07_RS02605 (MAFF301069_05080) - 562202..562861 (+) 660 WP_020832821.1 type 4a pilus biogenesis protein PilO -
  AACK07_RS02610 (MAFF301069_05090) - 562858..563403 (+) 546 WP_011002877.1 pilus assembly protein PilP -
  AACK07_RS02615 (MAFF301069_05100) pilQ 563406..565544 (+) 2139 WP_275541829.1 temperature dependent type IV pilus secretin PilQ Machinery gene
  AACK07_RS02620 (MAFF301069_05110) - 565686..566258 (+) 573 WP_011002875.1 shikimate kinase -
  AACK07_RS02625 (MAFF301069_05120) aroB 566255..567361 (+) 1107 WP_011002874.1 3-dehydroquinate synthase -
  AACK07_RS02630 (MAFF301069_05130) - 567381..568544 (+) 1164 WP_118871990.1 deoxyguanosinetriphosphate triphosphohydrolase -
  AACK07_RS02635 (MAFF301069_05140) - 568694..569368 (-) 675 WP_016727683.1 OmpW family protein -
  AACK07_RS02640 (MAFF301069_05150) - 569635..570360 (+) 726 WP_118871991.1 transposase -

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76379.47 Da        Isoelectric Point: 9.8259

>NTDB_id=97299 AACK07_RS02615 WP_275541829.1 563406..565544(+) (pilQ) [Ralstonia pseudosolanacearum strain MAFF 301069]
MRRVKGGLVQAVRVARGGAVAWLSLCMLMVGGQALAQQAITAPASNAVERVEQASTGESTVVTVTLKDTPAQKPVEFSTQ
QPARIAIDFFGTAFAQGRANYQYGGKLLRAASVVQIGDRTRVVLDLARQSQYKSEIRGNQYVLTLGAAPTASATPVPTFA
APAPTAGVERPSVRNIDFRRGEELAGRVVVDLSTSNSAINIAQQGQNLVVDFAGATLPQSLRRRFDVSDFGTPVQAMRAT
DNGTGARLVIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERMSLNFQNIDIRSLLQVFADFTNLNIVTSD
SVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRNELATKEKAELESQQQVTELEPLRSQVFRLNYQRADDV
RNMLLGTGGTAGGGAAASRILSKRGSLTSDARTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEADDTFSRNLG
AKLGFAAKTNGAGYGNTYSNVVSPVTKDATWDNSAAVSLPANGINGVNAASVAVSLFNAGAGRFLALELSALEADGRGKI
ISSPRVVTADNIKALIEQGTEVPYQQATSSGATSVSFKKANLKLEVTPKITPDGNVFLDVDVNKDSLGTQTTNGFAINTK
HVQTQVLVENGGTVVIGGIYTQNERTDVNKVPLLGDIPVLGNLFKSTAKTNDRTELLVFLTPRVLSDQLSLK

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=97299 AACK07_RS02615 WP_275541829.1 563406..565544(+) (pilQ) [Ralstonia pseudosolanacearum strain MAFF 301069]
ATGCGTCGCGTGAAGGGCGGCCTGGTGCAGGCCGTTCGAGTGGCCCGGGGGGGCGCAGTGGCATGGCTTTCGCTGTGTAT
GCTGATGGTGGGTGGACAGGCGTTGGCCCAGCAGGCAATCACGGCGCCGGCAAGCAATGCCGTCGAACGCGTGGAACAAG
CCTCGACAGGCGAATCCACCGTGGTGACGGTGACGCTGAAGGACACGCCCGCCCAGAAGCCGGTGGAATTCAGCACGCAG
CAGCCTGCGCGCATCGCCATCGACTTCTTCGGCACGGCATTCGCCCAGGGGCGTGCCAACTATCAATATGGCGGCAAGCT
GCTGCGCGCGGCCAGCGTGGTGCAGATTGGTGACCGCACCCGCGTCGTGCTGGATCTGGCGCGCCAGTCGCAGTACAAGT
CCGAGATCCGGGGCAACCAGTACGTGCTGACGCTGGGCGCCGCGCCGACGGCGTCGGCCACCCCGGTGCCGACCTTCGCC
GCGCCCGCGCCGACGGCCGGTGTGGAGCGGCCGTCCGTGCGCAACATCGACTTCCGCCGTGGCGAAGAGCTGGCCGGCCG
TGTGGTGGTCGACCTGTCGACGTCCAACTCCGCGATCAACATCGCTCAGCAGGGGCAAAACCTGGTGGTCGATTTCGCCG
GTGCCACGCTGCCGCAATCGCTGCGTCGCCGCTTTGACGTGAGCGACTTTGGCACGCCGGTGCAGGCCATGCGCGCCACC
GACAACGGCACGGGCGCTCGCCTGGTCATCGAGCCGCGCGGCAACTGGCAGTACAGCTCGTACCAGACCGACACGCAGTT
CGTGGTGGAAGTCCGCCCGACCAAGGAGGACCCGAACAAGCTGATCTCCGGTCCGGGCTATCGCGGGGAGCGCATGTCGC
TGAACTTCCAGAACATCGACATTCGTTCGCTGCTGCAGGTCTTTGCCGACTTCACCAACCTGAACATCGTCACCAGCGAC
AGCGTGACGGGGACCCTGTCGCTGCGTCTGAAGGACGTGCCGTGGGATCAGGCGCTGCAGATCGTGCTGGATTCCAAGGG
CCTGGCCTCGCGCCGCAACGGCAACGTGCTGTGGGTGGCGCCGCGCAACGAACTGGCGACCAAGGAGAAGGCTGAGCTGG
AGTCGCAGCAACAGGTGACCGAGCTGGAGCCGCTGCGCAGCCAGGTGTTCCGTCTGAATTACCAGCGTGCTGACGACGTG
CGCAACATGCTGCTGGGTACCGGAGGAACGGCGGGCGGTGGTGCTGCGGCGTCGCGCATCTTGTCCAAGCGTGGCTCGCT
GACGTCGGATGCCCGTACCAACCAGCTGTTCGTCTCGGATATCCCGAGCAAGCTGGAGGAGGTGCAGGCCTTCCTGCTGA
AGATTGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGATGACACGTTCAGCCGCAATCTTGGG
GCAAAGCTGGGCTTCGCGGCCAAGACCAATGGTGCCGGGTATGGCAACACCTACAGCAACGTGGTGTCGCCGGTCACGAA
AGACGCCACTTGGGATAACAGCGCGGCCGTCAGCCTGCCCGCCAATGGCATCAACGGTGTGAACGCCGCCAGTGTGGCAG
TGAGCTTGTTCAACGCCGGTGCCGGCCGTTTCCTGGCGCTCGAACTGTCGGCGCTGGAAGCCGATGGCCGCGGCAAGATC
ATCTCCAGCCCGCGTGTGGTCACCGCTGACAACATCAAGGCGCTGATCGAGCAGGGTACCGAAGTGCCTTACCAGCAGGC
GACCTCGTCGGGTGCGACGTCGGTTTCGTTCAAAAAGGCCAACCTGAAATTGGAGGTGACGCCGAAGATTACGCCGGACG
GCAACGTGTTCCTGGACGTGGACGTCAACAAGGACAGCCTGGGCACGCAGACCACCAACGGCTTCGCCATCAACACCAAG
CACGTGCAGACCCAGGTGCTGGTCGAGAACGGCGGCACGGTTGTGATTGGCGGCATCTACACGCAGAACGAACGGACCGA
TGTGAACAAGGTCCCGCTGCTGGGTGACATCCCGGTGCTGGGCAACCTGTTCAAGAGCACGGCCAAGACCAACGACCGGA
CGGAACTGCTGGTGTTCCTGACGCCGCGCGTGCTGTCCGACCAACTGTCGCTGAAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(551-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-461)

Secretin and TonB N terminus short domain (STN)

(316-361)

AMIN domain (AMIN)

(61-143)

AMIN domain (AMIN)

(195-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

41.467

93.82

0.389
  pilQ Neisseria gonorrhoeae strain FA1090

36.401

100

0.372
  comQ/pilQ Acinetobacter baylyi ADP1

38.15

97.191

0.371
  pilQ Legionella pneumophila strain Lp02

39.269

92.275

0.362
  pilQ Legionella pneumophila strain ERS1305867

39.269

92.275

0.362

Multiple sequence alignment