Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   WOC14_RS25400 Genome accession   NZ_CP150846
Coordinates   5553793..5555283 (+) Length   496 a.a.
NCBI ID   WP_085592358.1    Uniprot ID   A0AAJ5IGP5
Organism   Pseudomonas asiatica strain CJ23     
Function   require for natural transformation (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 5533672..5607826 5553793..5555283 within 0


Gene organization within MGE regions


Location: 5533672..5607826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC14_RS25300 ppk1 5535133..5537373 (-) 2241 WP_013974735.1 polyphosphate kinase 1 -
  WOC14_RS25305 - 5537587..5538231 (+) 645 WP_015272245.1 DedA family protein -
  WOC14_RS25310 - 5538781..5539359 (-) 579 Protein_4961 sterol desaturase family protein -
  WOC14_RS25315 elbB 5539472..5540131 (+) 660 WP_004375530.1 isoprenoid biosynthesis glyoxalase ElbB -
  WOC14_RS25320 - 5540285..5540737 (-) 453 WP_013974739.1 YaiI/YqxD family protein -
  WOC14_RS25325 - 5540910..5543765 (+) 2856 WP_406833742.1 class I adenylate cyclase -
  WOC14_RS25330 rnk 5543881..5544291 (-) 411 WP_023661830.1 nucleoside diphosphate kinase regulator -
  WOC14_RS25335 - 5544438..5544662 (-) 225 WP_013974742.1 DUF1289 domain-containing protein -
  WOC14_RS25340 cyaY 5544664..5544996 (-) 333 WP_236222898.1 iron donor protein CyaY -
  WOC14_RS25345 - 5545330..5545545 (+) 216 WP_004375542.1 lipoprotein -
  WOC14_RS25350 lysA 5545555..5546802 (+) 1248 WP_015272250.1 diaminopimelate decarboxylase -
  WOC14_RS25355 dapF 5546807..5547637 (+) 831 WP_013974744.1 diaminopimelate epimerase -
  WOC14_RS25360 - 5547651..5548361 (+) 711 WP_054573426.1 DUF484 family protein -
  WOC14_RS25365 xerC 5548361..5549260 (+) 900 WP_015272253.1 tyrosine recombinase XerC -
  WOC14_RS25370 - 5549257..5549952 (+) 696 WP_013974747.1 HAD family hydrolase -
  WOC14_RS25375 sutA 5550133..5550462 (-) 330 WP_013974748.1 transcriptional regulator SutA -
  WOC14_RS25380 - 5550892..5552223 (-) 1332 WP_015272254.1 ammonium transporter -
  WOC14_RS25385 glnK 5552270..5552608 (-) 339 WP_002555808.1 P-II family nitrogen regulator -
  WOC14_RS25390 - 5553010..5553276 (+) 267 WP_015272255.1 accessory factor UbiK family protein -
  WOC14_RS25395 - 5553277..5553630 (+) 354 WP_015272256.1 gamma-glutamylcyclotransferase family protein -
  WOC14_RS25400 comM 5553793..5555283 (+) 1491 WP_085592358.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  WOC14_RS25405 - 5555493..5555861 (-) 369 WP_015272300.1 response regulator transcription factor -
  WOC14_RS25410 - 5555858..5556850 (-) 993 WP_015272301.1 response regulator -
  WOC14_RS25415 - 5557089..5562137 (+) 5049 WP_406833743.1 AAA family ATPase -
  WOC14_RS25420 ycaC 5562192..5562818 (-) 627 WP_004375560.1 isochorismate family cysteine hydrolase YcaC -
  WOC14_RS25425 - 5562892..5563254 (-) 363 WP_015272303.1 AraC family transcriptional regulator -
  WOC14_RS25430 - 5563435..5564409 (+) 975 WP_043936302.1 GlxA family transcriptional regulator -
  WOC14_RS25435 - 5564591..5566378 (+) 1788 WP_085721334.1 monovalent cation:proton antiporter-2 (CPA2) family protein -
  WOC14_RS25440 - 5567096..5567389 (+) 294 WP_015272306.1 hypothetical protein -
  WOC14_RS25445 ycaC 5567567..5568196 (-) 630 WP_013974760.1 isochorismate family cysteine hydrolase YcaC -
  WOC14_RS25450 - 5568477..5569388 (+) 912 WP_015272307.1 LysR substrate-binding domain-containing protein -
  WOC14_RS25455 - 5569482..5570744 (+) 1263 WP_236222891.1 OprD family porin -
  WOC14_RS25460 - 5570746..5572344 (+) 1599 WP_406833744.1 MFS transporter -
  WOC14_RS25465 - 5572514..5574355 (-) 1842 WP_015272310.1 amidohydrolase -
  WOC14_RS25470 - 5574733..5575551 (-) 819 WP_085721331.1 alpha/beta fold hydrolase -
  WOC14_RS25475 - 5575591..5575899 (-) 309 WP_015272312.1 hypothetical protein -
  WOC14_RS25480 ycaC 5576014..5576643 (-) 630 WP_406833745.1 isochorismate family cysteine hydrolase YcaC -
  WOC14_RS25485 - 5576964..5578391 (+) 1428 WP_054572018.1 mechanosensitive ion channel family protein -
  WOC14_RS25490 - 5578494..5579780 (-) 1287 WP_013974769.1 FAD-binding oxidoreductase -
  WOC14_RS25495 - 5579904..5581394 (-) 1491 WP_003253547.1 aldehyde dehydrogenase family protein -
  WOC14_RS25500 - 5581531..5582421 (+) 891 WP_015272315.1 LysR family transcriptional regulator -
  WOC14_RS25505 - 5582643..5584028 (+) 1386 WP_406833746.1 VOC family protein -
  WOC14_RS25510 - 5584223..5585149 (-) 927 WP_015272317.1 LysR substrate-binding domain-containing protein -
  WOC14_RS25515 - 5585306..5586694 (+) 1389 WP_406833747.1 NorM family multidrug efflux MATE transporter -
  WOC14_RS25520 - 5586795..5588462 (-) 1668 WP_236222600.1 bifunctional diguanylate cyclase/phosphodiesterase -
  WOC14_RS25525 rep 5588716..5590725 (+) 2010 WP_015272320.1 DNA helicase Rep -
  WOC14_RS25530 - 5590791..5591363 (+) 573 WP_009683322.1 xanthine phosphoribosyltransferase -
  WOC14_RS25535 - 5591474..5593336 (-) 1863 WP_139657912.1 acetyl-CoA hydrolase/transferase C-terminal domain-containing protein -
  WOC14_RS25540 - 5593612..5594016 (-) 405 WP_038408395.1 cytochrome c5 family protein -
  WOC14_RS25545 - 5594171..5594719 (-) 549 WP_013974777.1 cupin domain-containing protein -
  WOC14_RS25550 alr 5594829..5595902 (-) 1074 WP_015272322.1 alanine racemase -
  WOC14_RS25555 dadA 5596051..5597355 (-) 1305 WP_013974779.1 D-amino acid dehydrogenase -
  WOC14_RS25560 dadR 5597512..5598000 (+) 489 WP_003258963.1 transcriptional regulator DadR -
  WOC14_RS25565 - 5598003..5598356 (-) 354 WP_013974780.1 YkgJ family cysteine cluster protein -
  WOC14_RS25570 - 5598556..5599848 (+) 1293 WP_015272323.1 FAD-binding oxidoreductase -
  WOC14_RS25575 - 5600004..5600234 (-) 231 WP_015272324.1 DUF1127 domain-containing protein -
  WOC14_RS25580 - 5600413..5601843 (+) 1431 WP_162491226.1 PLP-dependent aminotransferase family protein -
  WOC14_RS25585 - 5601904..5603460 (+) 1557 WP_015272325.1 phospholipase D family protein -
  WOC14_RS25590 - 5603582..5604721 (-) 1140 WP_015272326.1 MFS transporter -
  WOC14_RS25595 - 5604852..5606345 (-) 1494 WP_013974786.1 aldehyde dehydrogenase -
  WOC14_RS25600 - 5606542..5606901 (+) 360 WP_003258971.1 cupin domain-containing protein -
  WOC14_RS25605 rpmG 5606973..5607128 (-) 156 WP_003253507.1 50S ribosomal protein L33 -
  WOC14_RS25610 rpmB 5607139..5607375 (-) 237 WP_003258972.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 52808.51 Da        Isoelectric Point: 7.7255

>NTDB_id=972927 WOC14_RS25400 WP_085592358.1 5553793..5555283(+) (comM) [Pseudomonas asiatica strain CJ23]
MSLALVHSRAQVGVQAPAVSVETHLANGLPHLTLVGLPETTVKESKDRVRSAIVNSGLNYPQRRITQNLAPADLPKDGGR
YDLAIALGILAADGQVPTATLAEVECLGELALSGKLRPVQGVLPAALAAREAGRALVVPRENAEEASLAGGLVVYAVGHL
LELVAHLNGQVPLPPYAANGLILQQRPYPDLSEVQGQLAAKRALLLAAAGAHNLLFTGPPGTGKTLLASRLPGLLPPLDE
HEALEVAAIQSVSGHTPLSSWPQRPFRHPHHSASGPALVGGSSRPQPGEITLAHHGVLFLDELPEFERRVLEVLREPLES
GEIVIARARDKVRFPARFQLVAAMNPCPCGYLGDPTGRCRCSTEQIARYRNKLSGPLLDRIDLHLTVARESTTLNNQPCG
ETSADVAAKVAEARELQQRRQGCANAFLDLEGLRRHCGLAAADQAWLEGACERLTLSLRAAHRLLKVARTLADLEGSQAI
GRAHLAEALQYRPGSS

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=972927 WOC14_RS25400 WP_085592358.1 5553793..5555283(+) (comM) [Pseudomonas asiatica strain CJ23]
ATGTCCCTAGCCCTCGTCCACAGCCGCGCCCAGGTGGGCGTGCAGGCACCGGCGGTCAGCGTCGAAACCCACCTGGCCAA
CGGCTTGCCCCATCTCACGCTGGTCGGTCTGCCGGAAACCACGGTCAAAGAGAGCAAGGACCGGGTACGCAGCGCCATCG
TCAACTCCGGTTTGAACTACCCGCAGCGGCGCATCACCCAGAACCTCGCCCCCGCCGACCTGCCCAAGGATGGCGGGCGC
TATGACCTGGCAATTGCCCTGGGTATCCTCGCCGCCGATGGCCAGGTACCAACGGCCACCCTTGCCGAGGTCGAATGCCT
GGGCGAACTGGCGCTGTCTGGCAAGTTGCGCCCGGTGCAGGGCGTGTTGCCCGCAGCACTGGCGGCACGCGAAGCAGGCC
GGGCGCTGGTGGTGCCGCGGGAGAATGCCGAGGAAGCCAGCCTGGCAGGCGGGCTGGTGGTGTATGCGGTGGGGCACCTG
CTGGAACTGGTCGCCCACCTGAACGGCCAGGTGCCATTGCCACCGTATGCCGCCAACGGCCTGATCCTGCAGCAACGTCC
GTACCCGGACCTGAGCGAAGTGCAGGGCCAACTGGCGGCCAAGCGTGCGCTGCTGCTAGCGGCGGCCGGGGCGCACAACC
TTTTGTTCACCGGGCCTCCCGGTACCGGCAAGACCTTGCTTGCCAGCCGCTTGCCGGGGCTGCTGCCACCACTGGACGAG
CACGAAGCACTGGAAGTGGCGGCGATACAGTCGGTCAGCGGCCATACGCCACTGAGCAGCTGGCCGCAGCGGCCCTTCCG
CCATCCGCATCACTCCGCCTCCGGCCCGGCACTGGTCGGCGGCAGCAGCCGGCCGCAACCGGGCGAAATCACCCTCGCCC
ACCATGGCGTGCTGTTTCTGGATGAACTACCTGAGTTCGAGCGTCGCGTGCTGGAAGTGTTGCGCGAACCGCTGGAGTCG
GGCGAGATCGTGATTGCCCGGGCCCGCGACAAGGTGCGCTTCCCCGCACGTTTCCAGCTGGTGGCGGCAATGAACCCCTG
CCCTTGCGGCTATCTGGGCGACCCCACTGGCCGCTGCCGCTGCAGTACCGAGCAGATCGCGCGGTATCGCAACAAGCTGT
CCGGGCCGTTGCTGGACCGTATCGACCTGCACCTGACCGTGGCCCGGGAGAGCACCACGCTGAACAACCAGCCCTGCGGC
GAGACCAGTGCCGATGTGGCGGCCAAGGTAGCCGAGGCACGTGAACTGCAACAGCGGCGGCAAGGGTGTGCGAATGCGTT
TCTCGACCTTGAGGGGTTGCGCCGCCATTGTGGCTTGGCAGCGGCGGACCAGGCCTGGCTGGAGGGGGCATGTGAACGGC
TGACCCTGTCGTTGCGCGCGGCACACCGGTTGCTGAAGGTGGCGCGGACGCTGGCGGATCTGGAAGGTAGCCAGGCAATT
GGCCGGGCGCATCTGGCCGAGGCCCTGCAGTACCGGCCGGGGAGCAGTTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

55.6

100

0.56
  comM Vibrio campbellii strain DS40M4

55.758

99.798

0.556
  comM Vibrio cholerae strain A1552

55.152

99.798

0.55
  comM Glaesserella parasuis strain SC1401

53.892

100

0.544
  comM Legionella pneumophila str. Paris

49.395

100

0.494
  comM Legionella pneumophila strain ERS1305867

49.395

100

0.494
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

46.813

100

0.474

Multiple sequence alignment