Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACL60T_RS03465 Genome accession   NZ_CP178659
Coordinates   468342..469580 (+) Length   412 a.a.
NCBI ID   WP_000816000.1    Uniprot ID   -
Organism   Escherichia coli strain VTB96933v     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463342..474580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL60T_RS03440 (ACL60T_03440) mrcA 463622..466174 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  ACL60T_RS03445 (ACL60T_03445) hofM 466294..467073 (+) 780 WP_001469392.1 DNA utilization protein HofM -
  ACL60T_RS03450 (ACL60T_03450) hofN 467073..467612 (+) 540 WP_001069324.1 DNA utilization protein HofN -
  ACL60T_RS03455 (ACL60T_03455) hofO 467596..468036 (+) 441 WP_000367368.1 DNA utilization protein HofO -
  ACL60T_RS03460 (ACL60T_03460) hofP 468026..468430 (+) 405 WP_001604912.1 DNA utilization protein HofP -
  ACL60T_RS03465 (ACL60T_03465) comE 468342..469580 (+) 1239 WP_000816000.1 DNA uptake porin HofQ Machinery gene
  ACL60T_RS03470 (ACL60T_03470) aroK 469981..470502 (+) 522 WP_000818618.1 shikimate kinase AroK -
  ACL60T_RS03475 (ACL60T_03475) aroB 470559..471647 (+) 1089 WP_000439839.1 3-dehydroquinate synthase -
  ACL60T_RS03480 (ACL60T_03480) damX 471739..473025 (+) 1287 WP_000343195.1 cell division protein DamX -
  ACL60T_RS03485 (ACL60T_03485) dam 473132..473968 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44703.19 Da        Isoelectric Point: 6.1173

>NTDB_id=972700 ACL60T_RS03465 WP_000816000.1 468342..469580(+) (comE) [Escherichia coli strain VTB96933v]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=972700 ACL60T_RS03465 WP_000816000.1 468342..469580(+) (comE) [Escherichia coli strain VTB96933v]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACACTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACTAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCCGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGTGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGACAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362