Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACLEAH_RS27750 Genome accession   NZ_CP177242
Coordinates   5943960..5944583 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain SM45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5938960..5949583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLEAH_RS27730 (ACLEAH_27730) aroB 5939555..5940661 (-) 1107 WP_009314395.1 3-dehydroquinate synthase -
  ACLEAH_RS27735 (ACLEAH_27735) aroK 5940711..5941229 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACLEAH_RS27740 (ACLEAH_27740) pilQ 5941241..5943385 (-) 2145 WP_033995309.1 type 4a pilus secretin PilQ Machinery gene
  ACLEAH_RS27745 (ACLEAH_27745) comL/pilP 5943439..5943963 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACLEAH_RS27750 (ACLEAH_27750) pilO 5943960..5944583 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLEAH_RS27755 (ACLEAH_27755) pilN 5944580..5945176 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACLEAH_RS27760 (ACLEAH_27760) pilM 5945176..5946240 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACLEAH_RS27765 (ACLEAH_27765) - 5946425..5948896 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=970079 ACLEAH_RS27750 WP_003103268.1 5943960..5944583(-) (pilO) [Pseudomonas aeruginosa strain SM45]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=970079 ACLEAH_RS27750 WP_003103268.1 5943960..5944583(-) (pilO) [Pseudomonas aeruginosa strain SM45]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401