Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACLEAH_RS27755 Genome accession   NZ_CP177242
Coordinates   5944580..5945176 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain SM45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5939580..5950176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLEAH_RS27735 (ACLEAH_27735) aroK 5940711..5941229 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACLEAH_RS27740 (ACLEAH_27740) pilQ 5941241..5943385 (-) 2145 WP_033995309.1 type 4a pilus secretin PilQ Machinery gene
  ACLEAH_RS27745 (ACLEAH_27745) comL/pilP 5943439..5943963 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACLEAH_RS27750 (ACLEAH_27750) pilO 5943960..5944583 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLEAH_RS27755 (ACLEAH_27755) pilN 5944580..5945176 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACLEAH_RS27760 (ACLEAH_27760) pilM 5945176..5946240 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACLEAH_RS27765 (ACLEAH_27765) - 5946425..5948896 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=970080 ACLEAH_RS27755 WP_003095839.1 5944580..5945176(-) (pilN) [Pseudomonas aeruginosa strain SM45]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=970080 ACLEAH_RS27755 WP_003095839.1 5944580..5945176(-) (pilN) [Pseudomonas aeruginosa strain SM45]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTCGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369