Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHI53_RS00570 Genome accession   NZ_CP150304
Coordinates   108408..109796 (+) Length   462 a.a.
NCBI ID   WP_100530404.1    Uniprot ID   A0A2M9P229
Organism   Peribacillus sp. FSL E2-0218     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 103408..114796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI53_RS00550 (MHI53_00550) - 103534..103995 (+) 462 WP_061141076.1 CtsR family transcriptional regulator -
  MHI53_RS00555 (MHI53_00555) - 104017..104568 (+) 552 WP_061141077.1 UvrB/UvrC motif-containing protein -
  MHI53_RS00560 (MHI53_00560) - 104565..105641 (+) 1077 WP_061141078.1 protein arginine kinase -
  MHI53_RS00565 (MHI53_00565) clpC 105770..108211 (+) 2442 WP_340372566.1 ATP-dependent protease ATP-binding subunit ClpC -
  MHI53_RS00570 (MHI53_00570) radA 108408..109796 (+) 1389 WP_100530404.1 DNA repair protein RadA Machinery gene
  MHI53_RS00575 (MHI53_00575) disA 109799..110872 (+) 1074 WP_061141081.1 DNA integrity scanning diadenylate cyclase DisA -
  MHI53_RS00580 (MHI53_00580) - 111093..112187 (+) 1095 WP_061141082.1 PIN/TRAM domain-containing protein -
  MHI53_RS00585 (MHI53_00585) ispD 112208..112891 (+) 684 WP_061141083.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHI53_RS00590 (MHI53_00590) ispF 112906..113385 (+) 480 WP_061141084.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50228.79 Da        Isoelectric Point: 8.2404

>NTDB_id=970081 MHI53_RS00570 WP_100530404.1 108408..109796(+) (radA) [Peribacillus sp. FSL E2-0218]
MAVKKKTKFMCQSCGYESAKWMGKCPGCGEWNKMVEETEIVKPARKGAFTHSEVRTSGEREKAAPITTIQSEKEPRIKTD
LMELNRALGGGIVQGSLVLIGGDPGIGKSTLLLQVSSQLAHKQKKVLYISGEESVKQTKLRADRLGSMSENLYVYAETDM
DYIQQAISEVKPDLVIIDSIQTVYHSEVTSAPGSVSQVRECTASLMRIAKTNGVAIFIVGHVTKEGAIAGPRLLEHMVDT
VLYFEGERHHTYRIIRAVKNRFGSTNEMGIFEMKEHGLEEVANPSEIFLEERSQGASGSTVVASMEGTRPVLVEIQALIS
PTSFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAISIASSFRDKPTNPTDCIIGE
VGLTGEVRRVSRIEQRVQEAAKLGFNRVIIPANNIGGWTAPKGIKIVGVSSVSEALQHSLGG

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=970081 MHI53_RS00570 WP_100530404.1 108408..109796(+) (radA) [Peribacillus sp. FSL E2-0218]
ATGGCAGTGAAGAAGAAAACCAAATTCATGTGTCAGTCTTGCGGATATGAATCTGCGAAATGGATGGGGAAATGCCCAGG
CTGCGGTGAGTGGAACAAGATGGTCGAGGAAACTGAAATCGTGAAACCGGCGAGGAAAGGCGCCTTCACCCATTCGGAGG
TTAGAACCTCTGGGGAACGTGAGAAGGCTGCGCCGATAACGACCATTCAGTCTGAAAAAGAACCGCGGATCAAGACGGAT
TTAATGGAACTGAATCGTGCCCTTGGCGGCGGGATCGTCCAGGGGTCGCTTGTTTTGATTGGAGGTGATCCGGGTATCGG
GAAATCGACACTCCTTCTGCAGGTATCTTCCCAATTGGCACATAAGCAGAAAAAAGTGCTGTATATATCTGGTGAAGAAT
CAGTCAAGCAGACCAAATTGAGGGCCGACCGGCTGGGGAGCATGTCGGAAAACCTGTACGTGTATGCGGAAACGGATATG
GACTATATCCAACAGGCGATTTCAGAGGTGAAACCGGATTTGGTGATTATTGATTCCATCCAAACGGTGTACCATTCGGA
GGTTACATCTGCGCCGGGAAGTGTATCGCAAGTGCGCGAATGCACAGCTTCACTGATGCGCATCGCTAAAACGAATGGAG
TGGCTATTTTCATCGTCGGGCACGTAACGAAGGAAGGGGCGATTGCAGGCCCGCGTCTGCTTGAGCATATGGTGGACACC
GTATTATATTTCGAAGGCGAAAGGCACCATACATATCGAATTATCCGGGCGGTCAAAAACCGTTTTGGTTCGACGAATGA
GATGGGGATCTTTGAAATGAAAGAACATGGTTTGGAGGAAGTGGCCAATCCATCGGAAATATTCCTTGAAGAACGGTCCC
AAGGAGCGTCAGGTTCCACCGTCGTTGCCTCGATGGAAGGAACCAGGCCGGTGCTGGTAGAAATTCAGGCGTTGATTTCA
CCTACGAGCTTTGGAAATCCAAGGAGAATGGCGACAGGAATCGACCATAATCGTGTTTCGCTTCTCATGGCAGTCCTGGA
AAAAAGGGTGGGCTTGCTGCTGCAAAACCAGGATGCGTACTTAAAGGTGGCTGGCGGCGTTAAGCTCGATGAGCCGGCAA
TCGACCTGGCTGTCGCGATCAGCATCGCTTCGAGCTTCCGTGATAAACCAACGAACCCAACCGATTGCATCATTGGGGAA
GTGGGGCTCACAGGGGAAGTGAGAAGGGTTTCGCGAATAGAGCAAAGAGTGCAGGAAGCGGCAAAGCTTGGATTTAACCG
GGTGATAATTCCCGCTAATAATATCGGGGGATGGACCGCGCCGAAAGGTATCAAGATCGTCGGGGTTTCATCGGTTTCGG
AGGCTCTTCAACATTCGTTAGGGGGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(369-434)

AAA domain (AAA_25)

(77-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2M9P229

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.391

99.567

0.771
  radA Streptococcus mitis NCTC 12261

61.894

98.268

0.608
  radA Streptococcus mitis SK321

61.674

98.268

0.606
  radA Streptococcus pneumoniae TIGR4

61.454

98.268

0.604
  radA Streptococcus pneumoniae D39

61.454

98.268

0.604
  radA Streptococcus pneumoniae Rx1

61.454

98.268

0.604
  radA Streptococcus pneumoniae R6

61.454

98.268

0.604

Multiple sequence alignment