Detailed information NTG0969539

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MHB78_RS09225
• Genome accession: NZ_CP150286
• Coordinates: 1716639..1717262 (+)
• Length: 207 a.a.
• NCBI ID: WP_231947363.1
• Uniprot ID: -
• Organism: Bacillus sp. FSL K6-0138
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1711639..1722262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MHB78_RS09190 (MHB78_09190)	aroE	1712288..1713121 (+)	834	WP_046131757.1	shikimate dehydrogenase	-
MHB78_RS09195 (MHB78_09195)	yhbY	1713115..1713405 (+)	291	WP_046131758.1	ribosome assembly RNA-binding protein YhbY	-
MHB78_RS09200 (MHB78_09200)	-	1713424..1713993 (+)	570	WP_046131759.1	nicotinate-nucleotide adenylyltransferase	-
MHB78_RS09205 (MHB78_09205)	yqeK	1713983..1714546 (+)	564	WP_046131760.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MHB78_RS09210 (MHB78_09210)	rsfS	1714560..1714913 (+)	354	WP_046131761.1	ribosome silencing factor	-
MHB78_RS09215 (MHB78_09215)	-	1714913..1715668 (+)	756	WP_046131762.1	class I SAM-dependent methyltransferase	-
MHB78_RS09220 (MHB78_09220)	comER	1715736..1716557 (-)	822	WP_046131763.1	late competence protein ComER	-
MHB78_RS09225 (MHB78_09225)	comEA	1716639..1717262 (+)	624	WP_231947363.1	helix-hairpin-helix domain-containing protein	Machinery gene
MHB78_RS09230 (MHB78_09230)	-	1717331..1717900 (+)	570	WP_046131765.1	ComE operon protein 2	-
MHB78_RS09235 (MHB78_09235)	comEC	1717907..1720234 (+)	2328	WP_046131766.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MHB78_RS09240 (MHB78_09240)	-	1720337..1720468 (-)	132	WP_046131767.1	YqzM family protein	-
MHB78_RS09245 (MHB78_09245)	holA	1720698..1721726 (+)	1029	WP_164918190.1	DNA polymerase III subunit delta	-
MHB78_RS09250 (MHB78_09250)	rpsT	1721796..1722062 (-)	267	WP_046131769.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 207 a.a.
• Molecular weight: 21573.42 Da
• Isoelectric Point: 5.8770

>NTDB_id=969539 MHB78_RS09225 WP_231947363.1 1716639..1717262(+) (comEA) [Bacillus sp. FSL K6-0138]
MMNRLKRYKWPAAGGILAALFIIAVAVMSNIRSEPAGSNAVPAEAAASSSGRAAKKETKENDEIVIDLKGAVKKPGVYEM
KAGERVHHLLKKAGGTVKNAEEKQVNLAAVLQDGMVIYIPYEGEEGVQTGAGGGSSSTAGGEAETVNINTASSEELQAIP
GVGPSKAEAIAAYREENGPFQTVEDITNVSGIGEKTFEKIKSSISVK

Nucleotide

• Length: 624 bp

>NTDB_id=969539 MHB78_RS09225 WP_231947363.1 1716639..1717262(+) (comEA) [Bacillus sp. FSL K6-0138]
TTGATGAACAGGCTGAAGCGCTACAAATGGCCAGCGGCCGGAGGGATACTTGCAGCACTTTTTATCATCGCAGTCGCTGT
GATGTCAAACATACGCAGCGAACCAGCCGGGAGCAATGCTGTGCCGGCAGAGGCCGCCGCTTCTTCATCCGGCCGTGCTG
CCAAAAAGGAAACGAAAGAAAACGACGAAATTGTGATCGATCTGAAGGGCGCCGTCAAAAAGCCCGGCGTTTATGAGATG
AAGGCGGGCGAGAGGGTGCATCATTTGCTGAAAAAGGCGGGAGGTACCGTGAAAAATGCAGAAGAGAAACAAGTCAACCT
GGCCGCTGTCCTTCAGGATGGGATGGTGATTTATATTCCGTATGAAGGCGAGGAAGGCGTTCAAACCGGTGCGGGAGGAG
GATCATCATCGACAGCGGGAGGCGAAGCAGAAACGGTTAACATTAATACCGCTTCATCGGAAGAGCTTCAGGCGATCCCG
GGCGTCGGACCTTCAAAAGCGGAAGCGATCGCAGCATACCGTGAGGAGAACGGTCCGTTTCAAACGGTGGAAGACATTAC
AAATGTTTCAGGCATCGGAGAAAAAACATTTGAAAAAATAAAATCGTCAATATCAGTGAAGTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (66-120)

• Helix-hairpin-helix motif (HHH_3) (143-204)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	53.883	99.517	0.536
comEA	Latilactobacillus sakei subsp. sakei 23K	35.776	100	0.401
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.239	100	0.382
comEA/celA/cilE	Streptococcus pneumoniae D39	35.78	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.78	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	35.78	100	0.377
comEA	Lactococcus lactis subsp. cremoris KW2	34.884	100	0.362