Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MKZ05_RS16170 Genome accession   NZ_CP150255
Coordinates   3180302..3181426 (-) Length   374 a.a.
NCBI ID   WP_339160557.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0277     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3175302..3186426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ05_RS16135 (MKZ05_16135) flgL 3175482..3176393 (-) 912 WP_106030071.1 flagellar hook-associated protein FlgL -
  MKZ05_RS16140 (MKZ05_16140) flgK 3176403..3177923 (-) 1521 WP_003213544.1 flagellar hook-associated protein FlgK -
  MKZ05_RS16145 (MKZ05_16145) - 3177939..3178421 (-) 483 WP_050944342.1 flagellar protein FlgN -
  MKZ05_RS16150 (MKZ05_16150) flgM 3178440..3178703 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MKZ05_RS16155 (MKZ05_16155) - 3178783..3179202 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  MKZ05_RS16160 (MKZ05_16160) comFC 3179259..3179945 (-) 687 WP_212045588.1 ComF family protein Machinery gene
  MKZ05_RS16165 (MKZ05_16165) - 3179942..3180235 (-) 294 WP_106066158.1 late competence development ComFB family protein -
  MKZ05_RS16170 (MKZ05_16170) comFA 3180302..3181426 (-) 1125 WP_339160557.1 DEAD/DEAH box helicase Machinery gene
  MKZ05_RS16175 (MKZ05_16175) - 3181801..3182643 (-) 843 WP_226804196.1 DegV family protein -
  MKZ05_RS16180 (MKZ05_16180) degU 3182861..3183550 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MKZ05_RS16185 (MKZ05_16185) degS 3183615..3184790 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  MKZ05_RS16190 (MKZ05_16190) - 3185012..3185680 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42771.10 Da        Isoelectric Point: 10.4626

>NTDB_id=968102 MKZ05_RS16170 WP_339160557.1 3180302..3181426(-) (comFA) [Bacillus sp. FSL M8-0277]
MGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYALHQGMS
VCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDERLQFA
VLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKFKKNQLPPKVMNWMQKHITKK
RRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAESTIFT
ESALVQISGRAGRHPDFFKGDVFFFHFGLTRSMKQAMKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=968102 MKZ05_RS16170 WP_339160557.1 3180302..3181426(-) (comFA) [Bacillus sp. FSL M8-0277]
ATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATGTCACTCGGAATTAAC
ATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAAACAAATCTGATCTAC
TCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATATGCCTTACATCAAGGAATGAGT
GTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTTTCAAGGAATGAAAAT
AGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGTTAGTAATCGCTACAACTCACCAGTTGATGAGAT
ATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGAACGTCTCCAATTTGCT
GTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAATGATAAGAGATGTATC
TCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCATCCTTTCAATGGATTG
GACATTGGAAGAAGAAATTTAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAACATATTACAAAAAAG
AGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAAGCACCACTTAAATGT
AGAAGGGGTGTCTGCTGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTACAAATACGATGTACTAGTTA
CAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTGCAAGTGGGGGTACTAGGTGCAGAATCTACTATCTTTACA
GAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGTTTTCTTTTTTCATTT
TGGTTTAACGAGAAGTATGAAACAAGCAATGAAGCATATTGTAAAAATGAATGATACAGCTGAAAAAGAGTTCTCCGAAA
AATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(235-334)

Type III restriction enzyme, res subunit (ResIII)

(31-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

57.995

98.663

0.572
  comFA Latilactobacillus sakei subsp. sakei 23K

39.189

98.93

0.388
  comFA/cflA Streptococcus mitis SK321

40.395

94.652

0.382
  comFA/cflA Streptococcus pneumoniae Rx1

39.831

94.652

0.377
  comFA/cflA Streptococcus pneumoniae D39

39.831

94.652

0.377
  comFA/cflA Streptococcus pneumoniae R6

39.831

94.652

0.377
  comFA/cflA Streptococcus pneumoniae TIGR4

39.548

94.652

0.374
  comFA/cflA Streptococcus mitis NCTC 12261

39.266

94.652

0.372
  comFA Lactococcus lactis subsp. cremoris KW2

38.44

95.989

0.369

Multiple sequence alignment