Detailed information NTG0961986

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WKU41_RS02490
• Genome accession: NZ_CP149854
• Coordinates: 521842..522990 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2024CK-00032
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 516842..527990

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKU41_RS02465 (WKU41_02465)	-	517993..518289 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
WKU41_RS02470 (WKU41_02470)	-	518286..518879 (-)	594	WP_003084544.1	YggT family protein	-
WKU41_RS02475 (WKU41_02475)	proC	518890..519711 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
WKU41_RS02480 (WKU41_02480)	-	519723..520415 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
WKU41_RS02485 (WKU41_02485)	pilT	520630..521664 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WKU41_RS02490 (WKU41_02490)	pilU	521842..522990 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WKU41_RS02495 (WKU41_02495)	czcD	522998..523897 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
WKU41_RS02500 (WKU41_02500)	-	524051..524455 (+)	405	WP_003084560.1	NINE protein	-
WKU41_RS02505 (WKU41_02505)	-	524698..526071 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
WKU41_RS02510 (WKU41_02510)	-	526068..527252 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=961986 WKU41_RS02490 WP_003100959.1 521842..522990(+) (pilU) [Pseudomonas aeruginosa strain 2024CK-00032]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=961986 WKU41_RS02490 WP_003100959.1 521842..522990(+) (pilU) [Pseudomonas aeruginosa strain 2024CK-00032]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382