Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WKW49_RS13240 Genome accession   NZ_CP149819
Coordinates   3356464..3358191 (+) Length   575 a.a.
NCBI ID   WP_015818976.1    Uniprot ID   C5BP19
Organism   Teredinibacter turnerae strain SR02026     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3351464..3363191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKW49_RS13220 (WKW49_13220) - 3353234..3353767 (+) 534 WP_018015269.1 TIGR02281 family clan AA aspartic protease -
  WKW49_RS13225 (WKW49_13225) nadC 3353781..3354623 (+) 843 WP_339074026.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WKW49_RS13230 (WKW49_13230) - 3354689..3355429 (-) 741 WP_143876291.1 hypothetical protein -
  WKW49_RS13235 (WKW49_13235) - 3355565..3356065 (-) 501 WP_015818399.1 pilin -
  WKW49_RS13240 (WKW49_13240) pilB 3356464..3358191 (+) 1728 WP_015818976.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WKW49_RS13245 (WKW49_13245) pilC 3358195..3359409 (+) 1215 WP_339074027.1 type II secretion system F family protein Machinery gene
  WKW49_RS13250 (WKW49_13250) - 3359410..3360294 (+) 885 WP_339074028.1 A24 family peptidase -
  WKW49_RS13255 (WKW49_13255) coaE 3360294..3360902 (+) 609 WP_339074029.1 dephospho-CoA kinase -
  WKW49_RS13260 (WKW49_13260) yacG 3360892..3361092 (+) 201 WP_026160581.1 DNA gyrase inhibitor YacG -
  WKW49_RS13265 (WKW49_13265) - 3361086..3362045 (-) 960 WP_339074030.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62702.51 Da        Isoelectric Point: 5.0442

>NTDB_id=961715 WKW49_RS13240 WP_015818976.1 3356464..3358191(+) (pilB) [Teredinibacter turnerae strain SR02026]
MNSSANLSGLARRLVRDSLISEEDAQKAITGAAKDKIPLALYLVKNSSLDSRQIAESAADEFGTPLIDLQCINDTSSVKG
VVDAKLIHKHHALPLYKRGNRLFLALSDPTNLHALDEIKFNTGLNTEALLAEADKLHAAIEAFLHSEEEGIGDALGGLDE
EALDSLDIEAVDEDGGGSQEDASEADEAPIVRFVNKVLLDAIKLGASDIHFEPYEKAYRVRFRADGILKEVARPPVNLAT
RLAARLKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNSLPTLFGEKIVLRILDPSSAKLGIDALGYEEEQKKMYLDA
LAQPQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVNMNTKVGLTFAEALRSFLRQDPDIVMV
GEIRDLETAEIGIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPTFNVATTVSLIIAQRLARRLCSACKKPATDIPEEIL
NEEGFYNIGMEKNEITLFHPVGCDKCNEGYKGRVGIYEVVRITDEISRIIMEGGNSIQIADAARKAGFHDLRTSALRKAA
IGVTSLEEANRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=961715 WKW49_RS13240 WP_015818976.1 3356464..3358191(+) (pilB) [Teredinibacter turnerae strain SR02026]
ATGAATAGTTCAGCAAATCTCAGCGGATTGGCACGCCGACTCGTCCGAGACAGCCTCATCTCAGAAGAAGATGCGCAGAA
AGCCATTACCGGCGCAGCTAAAGACAAAATCCCGCTAGCGCTATACTTGGTCAAAAACAGTTCTCTTGATAGTCGGCAAA
TTGCGGAGTCCGCCGCGGACGAATTCGGTACACCACTCATTGATCTTCAGTGTATAAACGATACCTCTTCTGTCAAAGGT
GTAGTAGACGCCAAACTTATTCATAAACACCACGCATTACCCTTGTACAAGCGTGGCAATCGCTTGTTCCTTGCTCTTTC
AGACCCCACCAACCTTCATGCCCTTGACGAAATCAAATTTAACACAGGCCTGAACACCGAAGCGCTCTTGGCGGAAGCAG
ACAAGCTCCATGCTGCAATCGAAGCCTTTCTTCATTCTGAGGAAGAAGGAATCGGGGATGCGCTGGGAGGCCTGGACGAG
GAAGCACTAGACTCGCTTGATATCGAAGCAGTGGACGAGGACGGAGGCGGTAGCCAGGAGGACGCATCCGAGGCTGACGA
GGCTCCGATAGTGCGCTTTGTAAACAAAGTTCTGCTTGACGCCATCAAACTCGGCGCATCAGATATTCATTTTGAACCCT
ATGAAAAAGCCTACCGCGTTCGCTTTCGCGCAGACGGTATTCTGAAAGAGGTTGCCCGCCCTCCGGTCAACCTGGCCACT
CGCCTCGCCGCACGCCTTAAAGTCATGTCGCAAATGGATATTTCCGAGCGCAGGGTCCCTCAGGACGGCCGGATAAAGAT
GAAAATATCCAAAACCCGCGCGATTGATTTCCGGGTAAACAGCTTGCCGACGCTGTTTGGTGAAAAGATCGTTCTACGAA
TTTTGGACCCTTCAAGCGCGAAGCTCGGTATTGACGCCCTGGGTTACGAGGAAGAGCAGAAAAAAATGTACCTCGATGCG
TTGGCGCAACCACAGGGCATGATTCTGGTAACAGGCCCTACTGGCTCGGGCAAAACCGTATCGCTCTATACCGGTCTGAA
TATTCTAAATACCACCGAGCGCAACATATCCACCGCGGAAGATCCTGTAGAAATCAACCTGGAAGGCATCAACCAGGTCA
ATATGAACACTAAAGTCGGGCTTACGTTTGCAGAAGCACTGCGCTCATTCCTTCGGCAGGACCCTGATATCGTGATGGTC
GGGGAGATCCGGGACCTGGAAACCGCCGAAATCGGGATAAAAGCCGCGCAAACAGGTCACTTGGTGCTGTCGACCCTACA
CACCAACAGCGCCCCAGAAACGCTTACCCGACTGCTCAACATGGGCGTACCCACCTTCAACGTCGCCACAACCGTAAGTT
TGATCATTGCGCAACGATTGGCTCGGCGACTCTGCAGCGCCTGTAAAAAGCCAGCGACCGATATTCCTGAAGAAATTCTA
AATGAAGAAGGCTTCTATAATATTGGCATGGAAAAGAACGAAATCACACTTTTTCACCCTGTGGGTTGCGACAAATGCAA
TGAAGGTTATAAAGGTCGTGTGGGAATTTACGAGGTGGTGCGTATCACAGACGAAATCTCGCGAATTATCATGGAAGGCG
GCAATTCTATTCAAATTGCCGACGCGGCCAGGAAGGCAGGCTTTCACGATTTAAGAACCTCCGCCCTGCGAAAGGCGGCA
ATTGGCGTTACCAGCCTAGAAGAAGCAAACCGAGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C5BP19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.9

100

0.612
  pilB Acinetobacter baylyi ADP1

60

100

0.6
  pilB Legionella pneumophila strain ERS1305867

55.497

99.652

0.553
  pilB Vibrio cholerae strain A1552

51.323

98.609

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

50.348

99.826

0.503
  pilB Vibrio campbellii strain DS40M4

52.354

92.348

0.483
  pilF Neisseria gonorrhoeae MS11

47.986

99.304

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.147

93.739

0.367