Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WKI14_RS13640 Genome accession   NZ_CP149818
Coordinates   3465540..3467267 (+) Length   575 a.a.
NCBI ID   WP_015818976.1    Uniprot ID   C5BP19
Organism   Teredinibacter turnerae strain SR01903     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3460540..3472267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI14_RS13620 (WKI14_13620) - 3462324..3462857 (+) 534 WP_018015269.1 TIGR02281 family clan AA aspartic protease -
  WKI14_RS13625 (WKI14_13625) nadC 3462871..3463713 (+) 843 WP_339065240.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WKI14_RS13630 (WKI14_13630) - 3463765..3464505 (-) 741 WP_339065241.1 hypothetical protein -
  WKI14_RS13635 (WKI14_13635) - 3464641..3465141 (-) 501 WP_015818399.1 pilin -
  WKI14_RS13640 (WKI14_13640) pilB 3465540..3467267 (+) 1728 WP_015818976.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WKI14_RS13645 (WKI14_13645) pilC 3467271..3468485 (+) 1215 WP_339065244.1 type II secretion system F family protein Machinery gene
  WKI14_RS13650 (WKI14_13650) - 3468486..3469370 (+) 885 WP_339065246.1 A24 family peptidase -
  WKI14_RS13655 (WKI14_13655) coaE 3469370..3469978 (+) 609 WP_339065247.1 dephospho-CoA kinase -
  WKI14_RS13660 (WKI14_13660) yacG 3469968..3470168 (+) 201 WP_026160581.1 DNA gyrase inhibitor YacG -
  WKI14_RS13665 (WKI14_13665) - 3470162..3471121 (-) 960 WP_339065250.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62702.51 Da        Isoelectric Point: 5.0442

>NTDB_id=961693 WKI14_RS13640 WP_015818976.1 3465540..3467267(+) (pilB) [Teredinibacter turnerae strain SR01903]
MNSSANLSGLARRLVRDSLISEEDAQKAITGAAKDKIPLALYLVKNSSLDSRQIAESAADEFGTPLIDLQCINDTSSVKG
VVDAKLIHKHHALPLYKRGNRLFLALSDPTNLHALDEIKFNTGLNTEALLAEADKLHAAIEAFLHSEEEGIGDALGGLDE
EALDSLDIEAVDEDGGGSQEDASEADEAPIVRFVNKVLLDAIKLGASDIHFEPYEKAYRVRFRADGILKEVARPPVNLAT
RLAARLKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNSLPTLFGEKIVLRILDPSSAKLGIDALGYEEEQKKMYLDA
LAQPQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVNMNTKVGLTFAEALRSFLRQDPDIVMV
GEIRDLETAEIGIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPTFNVATTVSLIIAQRLARRLCSACKKPATDIPEEIL
NEEGFYNIGMEKNEITLFHPVGCDKCNEGYKGRVGIYEVVRITDEISRIIMEGGNSIQIADAARKAGFHDLRTSALRKAA
IGVTSLEEANRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=961693 WKI14_RS13640 WP_015818976.1 3465540..3467267(+) (pilB) [Teredinibacter turnerae strain SR01903]
ATGAATAGTTCAGCAAATCTCAGCGGATTGGCACGCCGACTCGTCCGAGACAGCCTCATCTCAGAAGAAGATGCGCAGAA
AGCCATTACCGGCGCAGCTAAAGACAAAATCCCGCTAGCGCTATACTTGGTCAAAAACAGTTCTCTTGATAGTCGGCAAA
TTGCGGAGTCCGCCGCGGACGAATTCGGTACACCACTCATTGATCTTCAGTGTATAAACGATACCTCTTCTGTCAAAGGT
GTAGTAGACGCCAAACTTATTCATAAACACCACGCATTACCCTTGTACAAGCGTGGCAATCGCTTGTTCCTTGCTCTTTC
AGACCCCACCAACCTTCATGCCCTTGACGAAATCAAATTTAACACAGGCCTGAACACCGAAGCGCTCTTGGCGGAAGCAG
ACAAGCTCCATGCTGCAATCGAAGCCTTTCTTCATTCTGAGGAAGAAGGAATCGGGGATGCGCTGGGAGGCCTGGACGAG
GAAGCATTAGACTCGCTTGATATCGAAGCAGTGGACGAGGACGGAGGCGGTAGCCAGGAGGACGCATCCGAGGCTGACGA
GGCTCCGATAGTGCGCTTTGTAAACAAAGTTCTGCTTGACGCTATCAAACTCGGCGCATCAGATATTCATTTTGAGCCCT
ATGAAAAAGCCTACCGCGTGCGCTTTCGCGCAGACGGTATTCTGAAAGAGGTTGCCCGCCCTCCGGTCAACCTGGCCACT
CGCCTGGCTGCACGCCTAAAAGTGATGTCACAAATGGATATTTCCGAGCGCAGGGTCCCTCAGGACGGCCGGATAAAGAT
GAAAATATCCAAAACCCGCGCGATTGATTTCCGGGTAAACAGCTTGCCGACGCTGTTTGGTGAAAAGATCGTTCTACGAA
TTTTGGATCCTTCAAGCGCGAAGCTCGGTATTGACGCCCTGGGTTACGAGGAAGAGCAGAAAAAAATGTACCTCGATGCG
TTGGCGCAACCACAGGGCATGATTCTGGTAACAGGCCCTACTGGCTCGGGCAAAACCGTATCGCTCTATACCGGTCTGAA
TATTCTAAATACCACCGAGCGCAACATATCCACCGCGGAAGATCCTGTAGAAATCAACCTGGAAGGCATCAACCAGGTCA
ATATGAACACTAAAGTCGGGCTTACGTTTGCAGAAGCACTGCGCTCATTCCTTCGGCAGGACCCTGATATCGTGATGGTC
GGGGAGATCCGGGACCTGGAAACCGCCGAAATCGGGATAAAAGCCGCGCAAACAGGTCACTTGGTGCTGTCGACCCTACA
CACCAACAGCGCCCCAGAAACGCTTACCCGACTGCTCAACATGGGCGTACCCACCTTCAACGTCGCCACAACCGTAAGTT
TGATCATTGCGCAACGATTGGCTCGGCGACTCTGCAGCGCCTGTAAAAAGCCAGCGACCGATATTCCTGAAGAAATTCTA
AATGAAGAAGGCTTCTATAATATTGGCATGGAAAAGAACGAAATCACACTTTTTCACCCTGTGGGTTGCGACAAATGCAA
TGAAGGTTATAAAGGTCGTGTGGGAATTTACGAGGTGGTGCGTATCACAGACGAAATCTCGCGAATTATCATGGAAGGCG
GCAATTCTATTCAAATTGCCGACGCGGCCAGAAAGGCAGGCTTTCACGATTTAAGAACCTCCGCCCTGCGAAAGGCGGCA
ATTGGCGTTACCAGCCTAGAAGAAGCAAACCGAGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C5BP19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.9

100

0.612
  pilB Acinetobacter baylyi ADP1

60

100

0.6
  pilB Legionella pneumophila strain ERS1305867

55.497

99.652

0.553
  pilB Vibrio cholerae strain A1552

51.323

98.609

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

50.348

99.826

0.503
  pilB Vibrio campbellii strain DS40M4

52.354

92.348

0.483
  pilF Neisseria gonorrhoeae MS11

47.986

99.304

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.147

93.739

0.367