Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WKI14_RS01000 Genome accession   NZ_CP149818
Coordinates   239676..240710 (+) Length   344 a.a.
NCBI ID   WP_015819744.1    Uniprot ID   C5BLK3
Organism   Teredinibacter turnerae strain SR01903     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 234676..245710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI14_RS00965 (WKI14_00965) - 234845..235282 (-) 438 WP_339066934.1 DUF4426 domain-containing protein -
  WKI14_RS00970 (WKI14_00970) metW 235279..235878 (-) 600 WP_015820259.1 methionine biosynthesis protein MetW -
  WKI14_RS00975 (WKI14_00975) - 235878..237029 (-) 1152 WP_015817511.1 homoserine O-acetyltransferase -
  WKI14_RS00980 (WKI14_00980) - 237038..237337 (-) 300 WP_015816900.1 DUF167 family protein -
  WKI14_RS00985 (WKI14_00985) - 237400..237987 (-) 588 WP_015818511.1 YggT family protein -
  WKI14_RS00990 (WKI14_00990) proC 238061..238894 (-) 834 WP_015820405.1 pyrroline-5-carboxylate reductase -
  WKI14_RS00995 (WKI14_00995) - 238944..239630 (-) 687 WP_015820845.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKI14_RS01000 (WKI14_01000) pilT 239676..240710 (+) 1035 WP_015819744.1 type IV pilus twitching motility protein PilT Machinery gene
  WKI14_RS01005 (WKI14_01005) pilU 240759..241901 (+) 1143 WP_015819970.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKI14_RS01010 (WKI14_01010) ruvX 241940..242380 (-) 441 WP_015820484.1 Holliday junction resolvase RuvX -
  WKI14_RS01015 (WKI14_01015) - 242373..242951 (-) 579 WP_015819114.1 YqgE/AlgH family protein -
  WKI14_RS01020 (WKI14_01020) - 243035..243916 (-) 882 WP_041590003.1 energy transducer TonB -
  WKI14_RS01025 (WKI14_01025) gshB 243916..244875 (-) 960 WP_339066943.1 glutathione synthase -
  WKI14_RS01030 (WKI14_01030) pilG 245122..245517 (+) 396 WP_015818072.1 twitching motility response regulator PilG Regulator

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38215.93 Da        Isoelectric Point: 6.5790

>NTDB_id=961679 WKI14_RS01000 WP_015819744.1 239676..240710(+) (pilT) [Teredinibacter turnerae strain SR01903]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGQVFRDVSSVPRGLVLVTGPTGSGKSTTLAAMMDYINENKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLEAVVSQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGGAIGMQTMDQCL
ADLVERRIISREVAREKAKMPDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=961679 WKI14_RS01000 WP_015819744.1 239676..240710(+) (pilT) [Teredinibacter turnerae strain SR01903]
ATGGATATTACCGAATTATTGGCGTTCTCAGCGAAGCAGGGCGCATCGGATTTACACCTCTCTGCTGGCTTGCCGCCAAT
GATCCGCGTCGATGGCGATGTGCGTCGTATCAACCTGCCGCCGATGGAGCACAAGCAGGTACACGGCCTCATCTACGAAA
TTATGAACGACAAGCAGCGCAAGGACTACGAGGAGTTCCTGGAAACGGATTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGGGTAAACGCATTTAACCAGAACCGTGGTGCAGGCGCGGTATTTCGAACGATCCCCTCCAAGGTGCTTACCATGGA
GGAACTGGGCATGGGGCAGGTCTTTCGCGATGTGTCCTCAGTGCCCCGCGGGTTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGTAAATCTACCACGCTTGCGGCGATGATGGACTACATCAACGAAAACAAATATCACCATATTCTGACTATTGAAGAC
CCGATAGAATTCGTGCACGAGAGCAAAAAGTGCCTGGTAAACCAGCGTGAAGTACACCGCGATACACATGGCTTTGCCGA
GGCGTTGCGCTCCGCGTTGCGGGAAGACCCGGACATTATTCTGGTGGGCGAGATGCGGGACCTTGAGACCATCCGATTGG
CGTTAACTGCCGCGGAAACCGGTCATTTGGTGTTCGGCACGCTGCATACCACTTCGGCGGCAAAAACCATCGACCGGGTT
GTCGACGTGTTTCCCGCCAATGAAAAAGCCATGGTGCGCTCAATGTTGTCGGAATCCCTGGAGGCGGTGGTTTCGCAAAC
ACTAATGAAAAAAAATGGCGGCGGGCGTGTTGCTGCACATGAAATCATGCGTGGCACACCGGCCATTCGCAACCTGATTC
GCGAAGACAAAGTGGCGCAAATGTACTCTGCTATTCAAACCGGCGGCGCCATCGGAATGCAAACGATGGATCAATGTCTG
GCGGATTTAGTCGAGCGCCGCATTATTTCGCGCGAAGTCGCGCGCGAAAAAGCCAAGATGCCGGACGCTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C5BLK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.826

100

0.898
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Neisseria meningitidis 8013

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

67.941

98.837

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

38.081

100

0.381