Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WKI13_RS07515 Genome accession   NZ_CP149817
Coordinates   1840581..1842308 (-) Length   575 a.a.
NCBI ID   WP_018276442.1    Uniprot ID   -
Organism   Teredinibacter turnerae strain T7902     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1835581..1847308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI13_RS07490 (WKI13_07490) - 1836700..1837683 (+) 984 WP_232427050.1 Nudix family hydrolase -
  WKI13_RS07495 (WKI13_07495) - 1837677..1837877 (-) 201 WP_018276438.1 DNA gyrase inhibitor YacG -
  WKI13_RS07500 (WKI13_07500) coaE 1837867..1838475 (-) 609 WP_018276439.1 dephospho-CoA kinase -
  WKI13_RS07505 (WKI13_07505) - 1838475..1839362 (-) 888 WP_018276440.1 A24 family peptidase -
  WKI13_RS07510 (WKI13_07510) pilC 1839363..1840577 (-) 1215 WP_018276441.1 type II secretion system F family protein Machinery gene
  WKI13_RS07515 (WKI13_07515) pilB 1840581..1842308 (-) 1728 WP_018276442.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WKI13_RS07520 (WKI13_07520) - 1842707..1843204 (+) 498 WP_018276444.1 pilin -
  WKI13_RS07525 (WKI13_07525) - 1843296..1844048 (+) 753 WP_018276445.1 hypothetical protein -
  WKI13_RS07530 (WKI13_07530) nadC 1844080..1844934 (-) 855 WP_018276446.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WKI13_RS07535 (WKI13_07535) - 1844948..1845439 (-) 492 WP_230515118.1 TIGR02281 family clan AA aspartic protease -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62697.42 Da        Isoelectric Point: 4.9839

>NTDB_id=961661 WKI13_RS07515 WP_018276442.1 1840581..1842308(-) (pilB) [Teredinibacter turnerae strain T7902]
MNSSANLSGLARRLVRDSLISEEDAQKAITGAAKDKIPLALYLVKNSSLDSRQIAESAADEFGTPLIDLQCINDTSSVKG
VVDAKLIHKHHALPLYKRGNRLFLALSDPTNLHALDEIKFNTGLNTEALLAEADKLHAAIEAFLHAEEEGIGDALGGLDE
EALDSLDIEAVDEDGGGSQDDTSEADEAPIVRFVNKVLLDAIKLGASDIHFEPYEKAYRVRFRADGILKEVARPPVNLAT
RLAARLKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNSLPTLFGEKIVLRILDPSSAKLGIDALGYEEEQKQMYLDA
LAQPQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVNMNTKVGLTFAEALRSFLRQDPDIVMV
GEIRDLETAEIGIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPTFNVATTVSLIIAQRLARRLCSSCKKPATDIPDEIL
HEEGFDNIGMEKNEITLFHPVGCDKCNEGYKGRVGIYEVVRITDEISRIIMEGGNSIQIADAARKAGFHDLRTSALRKAA
MGVTSLEEANRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=961661 WKI13_RS07515 WP_018276442.1 1840581..1842308(-) (pilB) [Teredinibacter turnerae strain T7902]
ATGAATAGTTCAGCAAATCTCAGCGGATTGGCACGCCGACTCGTCCGAGACAGCCTCATCTCAGAAGAAGATGCGCAGAA
AGCCATTACCGGCGCAGCTAAAGACAAAATCCCGCTAGCGCTATACTTGGTCAAAAACAGTTCTCTAGACAGTCGGCAAA
TTGCGGAGTCCGCCGCGGACGAATTCGGTACACCACTCATTGATCTTCAGTGTATAAACGATACCTCTTCTGTCAAAGGT
GTAGTAGACGCCAAACTTATTCATAAGCACCATGCATTGCCCTTATACAAGCGTGGCAATCGCTTGTTCCTTGCACTTTC
AGACCCCACCAACCTCCATGCCCTTGACGAAATCAAATTTAACACAGGTCTGAATACCGAAGCGCTTCTGGCTGAAGCAG
ATAAGCTTCATGCCGCGATTGAAGCCTTCCTTCATGCAGAGGAAGAGGGAATTGGGGATGCATTGGGTGGCCTGGACGAG
GAAGCGTTAGACTCGCTTGATATCGAAGCAGTTGACGAAGACGGAGGGGGTAGCCAGGACGACACATCCGAGGCTGACGA
GGCTCCGATAGTGCGCTTTGTAAACAAGGTTCTGCTTGACGCTATTAAGCTCGGCGCATCAGATATTCATTTTGAACCCT
ATGAAAAAGCCTACCGCGTACGATTTCGCGCAGACGGTATTCTGAAAGAGGTTGCCCGTCCTCCGGTCAACCTGGCCACT
CGCCTGGCGGCACGCTTAAAAGTGATGTCGCAAATGGATATTTCCGAGCGCAGAGTCCCTCAGGACGGCCGGATAAAGAT
GAAAATATCCAAAACCCGCGCGATTGATTTTCGGGTTAACAGCTTGCCAACGCTGTTTGGTGAGAAAATTGTTCTACGTA
TTTTGGACCCTTCAAGCGCGAAGCTCGGTATCGACGCCCTGGGTTACGAGGAAGAGCAGAAACAAATGTACCTCGATGCG
CTGGCGCAGCCTCAGGGTATGATTTTAGTCACAGGCCCGACAGGCTCAGGCAAAACCGTATCGCTCTATACCGGCCTGAA
TATTCTCAATACCACCGAGCGCAACATCTCTACCGCAGAAGATCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCA
ATATGAATACCAAAGTTGGGCTCACGTTCGCCGAAGCGCTGCGCTCGTTCCTTCGGCAAGACCCCGATATCGTAATGGTC
GGGGAGATCCGCGACCTGGAAACCGCCGAAATCGGCATTAAAGCCGCGCAAACAGGTCACTTAGTACTATCGACCCTACA
CACCAACAGCGCCCCAGAAACGCTTACCCGACTGCTCAACATGGGTGTACCCACCTTCAACGTCGCCACGACCGTGAGCT
TGATCATCGCACAGCGTCTCGCTCGTAGGCTTTGCAGCTCCTGCAAAAAACCCGCAACGGACATTCCCGACGAGATTTTG
CACGAGGAAGGCTTCGATAATATTGGCATGGAAAAGAACGAAATCACACTTTTTCACCCTGTGGGTTGCGACAAATGCAA
TGAAGGCTATAAAGGACGGGTGGGGATATACGAGGTGGTACGCATCACCGATGAAATCTCGCGAATTATCATGGAAGGCG
GCAATTCTATACAAATTGCCGACGCGGCGAGAAAGGCAGGCTTTCACGATTTAAGAACCTCTGCCTTACGAAAGGCGGCA
ATGGGCGTTACCAGCCTAGAAGAAGCAAACCGAGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.727

100

0.61
  pilB Acinetobacter baylyi ADP1

59.652

100

0.597
  pilB Legionella pneumophila strain ERS1305867

56.37

99.652

0.562
  pilB Vibrio cholerae strain A1552

51.323

98.609

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

50.523

99.826

0.504
  pilB Vibrio campbellii strain DS40M4

52.354

92.348

0.483
  pilF Neisseria gonorrhoeae MS11

47.986

99.304

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.776

93.739

0.363