Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI5E1_RS04675 Genome accession   NZ_CP173662
Coordinates   1041557..1042951 (-) Length   464 a.a.
NCBI ID   WP_237593620.1    Uniprot ID   A0A3G2M2T7
Organism   Actinobacillus pleuropneumoniae strain APL2305174E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1036557..1047951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI5E1_RS04640 (ACI5E1_04635) holA 1036702..1037727 (+) 1026 WP_237593618.1 DNA polymerase III subunit delta -
  ACI5E1_RS04645 (ACI5E1_04640) yacG 1037804..1037992 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  ACI5E1_RS04650 (ACI5E1_04645) coaE 1037974..1038612 (-) 639 WP_237593619.1 dephospho-CoA kinase -
  ACI5E1_RS04655 (ACI5E1_04650) - 1038634..1039272 (-) 639 WP_005604550.1 prepilin peptidase -
  ACI5E1_RS04660 (ACI5E1_04655) - 1039299..1039796 (-) 498 Protein_909 type II secretion system F family protein -
  ACI5E1_RS04665 (ACI5E1_04660) - 1039885..1040859 (+) 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
  ACI5E1_RS04670 (ACI5E1_04665) - 1040869..1041573 (-) 705 Protein_911 type II secretion system F family protein -
  ACI5E1_RS04675 (ACI5E1_04670) pilB 1041557..1042951 (-) 1395 WP_237593620.1 GspE/PulE family protein Machinery gene
  ACI5E1_RS04680 (ACI5E1_04675) pilA 1042969..1043415 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACI5E1_RS04685 (ACI5E1_04680) radA/sms 1043597..1044976 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  ACI5E1_RS04690 (ACI5E1_04685) - 1045020..1045253 (-) 234 WP_237593621.1 YgjV family protein -
  ACI5E1_RS04695 (ACI5E1_04690) folA 1045383..1045871 (-) 489 WP_237593622.1 type 3 dihydrofolate reductase -
  ACI5E1_RS04700 (ACI5E1_04695) proB 1045958..1047049 (+) 1092 WP_237593623.1 glutamate 5-kinase -
  ACI5E1_RS04705 (ACI5E1_04700) - 1047062..1047838 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53080.93 Da        Isoelectric Point: 6.3752

>NTDB_id=961623 ACI5E1_RS04675 WP_237593620.1 1041557..1042951(-) (pilB) [Actinobacillus pleuropneumoniae strain APL2305174E]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQEQHSDVNDPIIQVLDGLFKYGLKHNASDIHLEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=961623 ACI5E1_RS04675 WP_237593620.1 1041557..1042951(-) (pilB) [Actinobacillus pleuropneumoniae strain APL2305174E]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTTCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGAGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTGGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G2M2T7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.41

98.922

0.677
  pilB Haemophilus influenzae Rd KW20

59.341

98.06

0.582
  pilB Haemophilus influenzae 86-028NP

58.901

98.06

0.578
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.412
  pilB Acinetobacter baumannii D1279779

39.059

100

0.412
  pilB Vibrio cholerae strain A1552

39.212

100

0.407
  pilB Acinetobacter baylyi ADP1

38.115

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369