Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACIRXM_RS05615 Genome accession   NZ_CP173234
Coordinates   1089016..1090338 (+) Length   440 a.a.
NCBI ID   WP_035688106.1    Uniprot ID   A0A0F5EWW2
Organism   Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1084016..1095338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIRXM_RS05590 (ACIRXM_05595) - 1084117..1086666 (-) 2550 WP_410687690.1 penicillin-binding protein 1A -
  ACIRXM_RS05595 (ACIRXM_05600) comA 1086825..1087592 (+) 768 WP_264245250.1 hypothetical protein Machinery gene
  ACIRXM_RS05600 (ACIRXM_05605) - 1087622..1088134 (+) 513 WP_017806830.1 PilN domain-containing protein -
  ACIRXM_RS05605 (ACIRXM_05610) - 1088121..1088648 (+) 528 WP_410683657.1 hypothetical protein -
  ACIRXM_RS05610 (ACIRXM_05615) - 1088648..1089007 (+) 360 WP_410687692.1 hypothetical protein -
  ACIRXM_RS05615 (ACIRXM_05620) comE 1089016..1090338 (+) 1323 WP_035688106.1 type IV pilus secretin PilQ Machinery gene
  ACIRXM_RS05620 (ACIRXM_05625) aroK 1090539..1091099 (+) 561 WP_272487554.1 shikimate kinase AroK -
  ACIRXM_RS05625 (ACIRXM_05630) aroB 1091116..1092204 (+) 1089 WP_410687694.1 3-dehydroquinate synthase -
  ACIRXM_RS05630 (ACIRXM_05635) - 1092210..1093100 (+) 891 WP_035688112.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACIRXM_RS05635 (ACIRXM_05640) - 1093072..1093377 (-) 306 WP_035688115.1 DciA family protein -
  ACIRXM_RS05640 (ACIRXM_05645) secM 1093483..1093794 (+) 312 WP_026138577.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 48606.59 Da        Isoelectric Point: 6.1204

>NTDB_id=957719 ACIRXM_RS05615 WP_035688106.1 1089016..1090338(+) (comE) [Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1]
MKLPNIIKNILLFFLILSYPFRQVNASDENNTFSVHLKQAPLVDTVQYLAGYQKINLVIDDELSGSISLQLQNTTLLQLL
KSIAEIKHLRFWENNGIYYLSQSQATNQENVVLNSSTEIATSADMLITSIKLHYAKASEVMKALTSGNGSLLTANGSISF
DERNNLLIIQDEAKKIKQIRKLVADMDKPIQQIIIEARIVTISDESLKELGVRWGIFSPSDISHSVSGSLNANGFSNITD
NLNINFATSSNPAGSVALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTEIPYVVNNGKNDTQSIE
FREAVLGLEVTPHLLKHNGILLDLVVSQNAPGGRVSYGENEVISIDKQEINTQVFAQDGETIVLGGIFHDTITKALDKVP
ILGDIPGIKRLFSKEVERHQKRELVIFVTPRLIRSGTVEE

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=957719 ACIRXM_RS05615 WP_035688106.1 1089016..1090338(+) (comE) [Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1]
ATGAAGTTACCAAATATAATAAAGAATATTCTTTTATTTTTTCTTATTCTATCCTATCCATTTAGACAGGTTAATGCAAG
TGACGAAAATAATACATTCTCTGTTCATTTAAAGCAGGCTCCATTAGTAGATACTGTACAATATTTAGCTGGGTATCAAA
AAATCAATTTAGTCATAGATGATGAATTGTCTGGTTCAATATCTTTGCAATTGCAAAATACAACATTACTTCAATTATTA
AAATCTATCGCTGAAATCAAACATTTGCGGTTTTGGGAAAATAATGGGATATATTATTTAAGTCAGTCGCAAGCAACTAA
TCAAGAAAATGTAGTGTTAAATTCATCAACAGAAATAGCAACATCAGCAGATATGTTAATAACTTCAATAAAGTTGCACT
ATGCCAAAGCATCCGAGGTAATGAAAGCCCTCACTTCAGGGAATGGCTCTTTATTAACAGCAAATGGAAGTATAAGTTTT
GATGAACGCAATAATTTACTCATTATCCAAGATGAAGCTAAAAAAATAAAACAAATTAGGAAATTGGTAGCGGATATGGA
TAAACCCATTCAGCAAATTATTATTGAAGCTCGTATTGTCACAATTAGCGATGAAAGTTTAAAAGAATTGGGAGTCAGAT
GGGGAATTTTTTCTCCCTCAGATATAAGCCATAGCGTTTCTGGAAGTTTAAATGCAAATGGATTCTCAAATATTACGGAT
AATCTAAATATTAATTTTGCGACGAGTTCAAATCCTGCAGGTTCGGTAGCTTTGCAAGTAGCTAAAATTAATGGAAGGTT
ATTAGATTTAGAGTTGACAGCATTAGAAAGAGAAAATAACGTTGAAATTATTGCGAGTCCACGATTATTGACCACAAATA
AGAAAAGTGCAAGTATCAAGCAAGGTACAGAAATCCCCTATGTAGTGAATAATGGAAAAAATGATACGCAGTCTATTGAA
TTCCGAGAAGCTGTACTTGGGCTTGAAGTTACACCTCATTTATTAAAGCATAACGGTATTTTACTTGATCTTGTTGTTAG
CCAGAATGCACCTGGAGGACGGGTATCTTATGGAGAAAATGAGGTGATTTCTATTGATAAACAGGAAATTAATACGCAAG
TCTTTGCACAAGACGGCGAAACGATTGTATTGGGCGGAATTTTCCACGATACGATTACTAAAGCACTTGATAAAGTACCA
ATTTTAGGAGACATACCTGGAATAAAAAGGCTATTTAGTAAGGAAGTTGAGAGACATCAAAAGAGGGAACTCGTTATTTT
TGTTACACCACGCTTAATTCGTAGTGGTACAGTAGAGGAATGA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(129-192)

Bacterial type II and III secretion system protein (Secretin)

(274-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5EWW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

65.023

96.818

0.63
  comE Haemophilus influenzae 86-028NP

64.789

96.818

0.627
  comE Glaesserella parasuis strain SC1401

49.883

97.045

0.484
  pilQ Vibrio campbellii strain DS40M4

40

96.591

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.288

94.773

0.382
  pilQ Vibrio cholerae strain A1552

40.288

94.773

0.382