Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   WHU13_RS22965 Genome accession   NZ_CP148366
Coordinates   4696547..4698067 (-) Length   506 a.a.
NCBI ID   WP_280225329.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323HEC0300     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4691547..4703067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU13_RS22955 (WHU13_22960) hdfR 4695226..4696065 (-) 840 WP_000379257.1 HTH-type transcriptional regulator HdfR -
  WHU13_RS22960 (WHU13_22965) maoP 4696184..4696522 (+) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  WHU13_RS22965 (WHU13_22970) comM 4696547..4698067 (-) 1521 WP_280225329.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  WHU13_RS22970 (WHU13_22975) ilvL 4698420..4698518 (+) 99 WP_001311244.1 ilv operon leader peptide -
  WHU13_RS22975 (WHU13_22980) ilvX 4698605..4698655 (+) 51 WP_001387183.1 peptide IlvX -
  WHU13_RS22980 (WHU13_22985) ilvG 4698658..4700304 (+) 1647 WP_001012595.1 acetolactate synthase 2 catalytic subunit -
  WHU13_RS22985 (WHU13_22990) ilvM 4700301..4700564 (+) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  WHU13_RS22990 (WHU13_22995) ilvE 4700584..4701513 (+) 930 WP_000208520.1 branched-chain-amino-acid transaminase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55264.43 Da        Isoelectric Point: 7.9062

>NTDB_id=954041 WHU13_RS22965 WP_280225329.1 4696547..4698067(-) (comM) [Escherichia coli strain Z1323HEC0300]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQKAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=954041 WHU13_RS22965 WP_280225329.1 4696547..4698067(-) (comM) [Escherichia coli strain Z1323HEC0300]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCAGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTGCCTATCGCTATTGCGTTACTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCAATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAACAGCACCACCGTTAAACAACGTGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAATCCGGAAATACGCCAATTCTGCAAGCTTGAAAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGAAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.121

100

0.587
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio cholerae strain A1552

58.566

99.209

0.581
  comM Vibrio campbellii strain DS40M4

57.57

99.209

0.571
  comM Legionella pneumophila str. Paris

47.791

98.419

0.47
  comM Legionella pneumophila strain ERS1305867

47.791

98.419

0.47
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

43.874

100

0.439