Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NGK73_RS20055 Genome accession   NZ_AP025915
Coordinates   4148508..4149488 (-) Length   326 a.a.
NCBI ID   WP_017692691.1    Uniprot ID   A0A2J0PYR2
Organism   Enterobacter hormaechei strain FUJ80377     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4143508..4154488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK73_RS20025 (FJMB80377_39210) - 4144386..4144883 (+) 498 WP_032649479.1 SprT family zinc-dependent metalloprotease -
  NGK73_RS20030 (FJMB80377_39220) endA 4144978..4145685 (+) 708 WP_003860034.1 deoxyribonuclease I -
  NGK73_RS20035 (FJMB80377_39230) rsmE 4145737..4146468 (+) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NGK73_RS20040 (FJMB80377_39240) gshB 4146500..4147447 (+) 948 WP_015571835.1 glutathione synthase -
  NGK73_RS20045 (FJMB80377_39250) - 4147522..4148082 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  NGK73_RS20050 (FJMB80377_39260) ruvX 4148082..4148498 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  NGK73_RS20055 (FJMB80377_39270) pilT 4148508..4149488 (-) 981 WP_017692691.1 type IV pilus twitching motility protein PilT Machinery gene
  NGK73_RS20060 (FJMB80377_39280) - 4149506..4150207 (+) 702 WP_032649482.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGK73_RS20065 (FJMB80377_39290) - 4150229..4150795 (+) 567 WP_015571838.1 YggT family protein -
  NGK73_RS20070 (FJMB80377_39300) yggU 4150792..4151088 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  NGK73_RS20075 (FJMB80377_39310) - 4151092..4151685 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  NGK73_RS20080 (FJMB80377_39320) hemW 4151678..4152826 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  NGK73_RS20085 (FJMB80377_39330) - 4152883..4153245 (+) 363 WP_032649485.1 endonuclease domain-containing protein -
  NGK73_RS20090 (FJMB80377_39340) - 4153405..4154121 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35682.70 Da        Isoelectric Point: 6.3047

>NTDB_id=95077 NGK73_RS20055 WP_017692691.1 4148508..4149488(-) (pilT) [Enterobacter hormaechei strain FUJ80377]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=95077 NGK73_RS20055 WP_017692691.1 4148508..4149488(-) (pilT) [Enterobacter hormaechei strain FUJ80377]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAAGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCAGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J0PYR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment