Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACHP5S_RS18780 Genome accession   NZ_CP172127
Coordinates   3884651..3885889 (+) Length   412 a.a.
NCBI ID   WP_096983595.1    Uniprot ID   -
Organism   Escherichia coli strain GF43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3879651..3890889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5S_RS18755 (ACHP5S_18755) mrcA 3879931..3882483 (-) 2553 WP_042002839.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  ACHP5S_RS18760 (ACHP5S_18760) hofM 3882603..3883382 (+) 780 WP_096983599.1 DNA utilization protein HofM -
  ACHP5S_RS18765 (ACHP5S_18765) hofN 3883382..3883921 (+) 540 WP_096983598.1 DNA utilization protein HofN -
  ACHP5S_RS18770 (ACHP5S_18770) hofO 3883905..3884345 (+) 441 WP_096983597.1 DNA utilization protein HofO -
  ACHP5S_RS18775 (ACHP5S_18775) hofP 3884335..3884739 (+) 405 WP_096983596.1 DNA utilization protein HofP -
  ACHP5S_RS18780 (ACHP5S_18780) comE 3884651..3885889 (+) 1239 WP_096983595.1 DNA uptake porin HofQ Machinery gene
  ACHP5S_RS18785 (ACHP5S_18785) aroK 3886290..3886811 (+) 522 WP_000818618.1 shikimate kinase AroK -
  ACHP5S_RS18790 (ACHP5S_18790) aroB 3886868..3887956 (+) 1089 WP_042002841.1 3-dehydroquinate synthase -
  ACHP5S_RS18795 (ACHP5S_18795) damX 3888048..3889334 (+) 1287 WP_096983594.1 cell division protein DamX -
  ACHP5S_RS18800 (ACHP5S_18800) dam 3889441..3890277 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44720.26 Da        Isoelectric Point: 5.9488

>NTDB_id=950175 ACHP5S_RS18780 WP_096983595.1 3884651..3885889(+) (comE) [Escherichia coli strain GF43]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELLAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=950175 ACHP5S_RS18780 WP_096983595.1 3884651..3885889(+) (comE) [Escherichia coli strain GF43]
ATGAAGCAATGGATAGCAGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACACTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCCGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTTGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGTGGCATTTTTACCCGTAAAAATAAATCGGGGCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362