Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WAE38_RS08035 Genome accession   NZ_CP147984
Coordinates   1623249..1623707 (+) Length   152 a.a.
NCBI ID   WP_075605061.1    Uniprot ID   K4JZ52
Organism   Glaesserella parasuis strain H16SD2BY4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1618249..1628707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAE38_RS08015 (WAE38_08015) - 1618639..1620093 (-) 1455 WP_338823446.1 aminoacyl-histidine dipeptidase -
  WAE38_RS08020 (WAE38_08020) gpt 1620273..1620752 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  WAE38_RS08025 (WAE38_08025) gmk 1621006..1621632 (+) 627 WP_261473486.1 guanylate kinase -
  WAE38_RS08030 (WAE38_08030) radA 1621685..1623061 (-) 1377 WP_005711784.1 DNA repair protein RadA -
  WAE38_RS08035 (WAE38_08035) pilA 1623249..1623707 (+) 459 WP_075605061.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  WAE38_RS08040 (WAE38_08040) pilB 1623755..1625140 (+) 1386 WP_203399225.1 GspE/PulE family protein Machinery gene
  WAE38_RS08045 (WAE38_08045) pilC 1625133..1626329 (+) 1197 WP_203399224.1 type II secretion system F family protein Machinery gene
  WAE38_RS08050 (WAE38_08050) - 1626326..1626994 (+) 669 WP_035491631.1 prepilin peptidase -
  WAE38_RS08055 (WAE38_08055) dapE 1627078..1628211 (+) 1134 WP_021113185.1 succinyl-diaminopimelate desuccinylase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16062.55 Da        Isoelectric Point: 9.0925

>NTDB_id=949838 WAE38_RS08035 WP_075605061.1 1623249..1623707(+) (pilA) [Glaesserella parasuis strain H16SD2BY4]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNTGALTNCTGGSNGV
QANKTDVADAKYLKSISVASGTITVEGKGSIDGYGYTMTPKFANNNITWATSCRGSDISLFPANFCGTKATQ

Nucleotide


Download         Length: 459 bp        

>NTDB_id=949838 WAE38_RS08035 WP_075605061.1 1623249..1623707(+) (pilA) [Glaesserella parasuis strain H16SD2BY4]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCTGCACTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACACAGGGGCTTTAACTAACTGTACAGGGGGTTCTAACGGCGTT
CAAGCCAATAAAACCGATGTTGCCGATGCTAAATATCTTAAATCTATTTCCGTCGCTTCGGGGACAATCACCGTAGAAGG
CAAAGGCAGCATTGATGGCTACGGCTACACAATGACCCCGAAATTTGCCAATAACAACATTACTTGGGCAACAAGCTGTC
GAGGTTCTGACATTAGTTTATTCCCTGCGAATTTCTGTGGGACAAAAGCCACACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4JZ52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

86.928

100

0.875
  pilA Haemophilus influenzae 86-028NP

57.432

97.368

0.559
  pilA Haemophilus influenzae Rd KW20

57.432

97.368

0.559
  comP Acinetobacter baylyi ADP1

43.077

85.526

0.368