Detailed information NTG0948197

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WDW98_RS05475
• Genome accession: NZ_CP147720
• Coordinates: 1142710..1143852 (+)
• Length: 380 a.a.
• NCBI ID: WP_005408369.1
• Uniprot ID: A0A854DW57
• Organism: Stenotrophomonas maltophilia strain CGMCC 1.1788
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1137710..1148852

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WDW98_RS05450	-	1138214..1138825 (-)	612	Protein_1057	short chain dehydrogenase	-
WDW98_RS05455	-	1138915..1139790 (+)	876	WP_024956082.1	LysR family transcriptional regulator	-
WDW98_RS05460	proC	1139967..1140788 (-)	822	WP_024956081.1	pyrroline-5-carboxylate reductase	-
WDW98_RS05465	-	1140807..1141484 (-)	678	WP_024956080.1	YggS family pyridoxal phosphate-dependent enzyme	-
WDW98_RS05470	pilT	1141572..1142609 (+)	1038	WP_004147099.1	type IV pilus twitching motility protein PilT	Machinery gene
WDW98_RS05475	pilU	1142710..1143852 (+)	1143	WP_005408369.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WDW98_RS05480	-	1144060..1145043 (+)	984	WP_024956079.1	aldo/keto reductase	-
WDW98_RS05485	-	1145091..1145990 (-)	900	WP_024956078.1	LysR family transcriptional regulator	-
WDW98_RS05490	-	1146084..1146293 (+)	210	WP_005412490.1	DUF4287 domain-containing protein	-
WDW98_RS05495	-	1146404..1147111 (+)	708	WP_024956077.1	YitT family protein	-
WDW98_RS05500	-	1147192..1147737 (-)	546	WP_024956076.1	DNA-3-methyladenine glycosylase I	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42355.69 Da
• Isoelectric Point: 6.7683

>NTDB_id=948197 WDW98_RS05475 WP_005408369.1 1142710..1143852(+) (pilU) [Stenotrophomonas maltophilia strain CGMCC 1.1788]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide

• Length: 1143 bp

>NTDB_id=948197 WDW98_RS05475 WP_005408369.1 1142710..1143852(+) (pilU) [Stenotrophomonas maltophilia strain CGMCC 1.1788]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGTGAGGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAGACCGGTCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGAAAGGTCGCCATGGAGATCCTGCTCGGCA
CGCCGCTGGTTCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGCACGCTGTCACAGGGCCTGGATGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-277)

3D structure

• AlphaFold DB: A0A854DW57

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.847	96.579	0.655
pilU	Acinetobacter baylyi ADP1	63.585	93.947	0.597
pilU	Vibrio cholerae strain A1552	53.371	93.684	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.099	93.421	0.403