Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   NGK28_RS21925 Genome accession   NZ_AP025821
Coordinates   4577614..4579134 (+) Length   506 a.a.
NCBI ID   WP_022649778.1    Uniprot ID   A0A3S0HFG8
Organism   Enterobacter hormaechei strain FUJ80066     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4572614..4584134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK28_RS21900 (FJMB80066_42860) ilvE 4574180..4575109 (-) 930 WP_022649776.1 branched-chain-amino-acid transaminase -
  NGK28_RS21905 (FJMB80066_42870) ilvM 4575128..4575391 (-) 264 WP_251265452.1 acetolactate synthase 2 small subunit -
  NGK28_RS21910 (FJMB80066_42880) ilvG 4575388..4577034 (-) 1647 WP_022649777.1 acetolactate synthase 2 catalytic subunit -
  NGK28_RS21915 ilvX 4577037..4577087 (-) 51 WP_166792073.1 peptide IlvX -
  NGK28_RS21920 ilvL 4577174..4577272 (-) 99 WP_001311244.1 ilv operon leader peptide -
  NGK28_RS21925 (FJMB80066_42890) comM 4577614..4579134 (+) 1521 WP_022649778.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  NGK28_RS21930 (FJMB80066_42900) - 4579161..4579499 (-) 339 WP_006808641.1 DUF413 domain-containing protein -
  NGK28_RS21935 (FJMB80066_42910) hdfR 4579618..4580439 (+) 822 WP_022649779.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 54732.74 Da        Isoelectric Point: 7.3360

>NTDB_id=94513 NGK28_RS21925 WP_022649778.1 4577614..4579134(+) (comM) [Enterobacter hormaechei strain FUJ80066]
MSLSVVYTRAAIGVKAPLISVEVHLSNGLPGLTLVGLPETTVKEARDRVRSAIINSGYAFPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLNTPGLSSCEFVGELALTGALRGVPGAISGALEAIRAGRQIIVANENASEVSLIAEKGCLVAGHL
QEVCAWLEGRHELAEPQENEEVVADSPDDLSDIMGQEQGKRALEITAAGGHNLLLIGPPGTGKTMLASRLSGLLPPLNNH
EALESAAIFSLVSSISLHKQWRRRPFRSPHHSASLTAMVGGGSIPGPGEISLAHNGILFLDELPEFERRVLDALREPIES
GEIHLSRTRAKISYPAQFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLSKLSGPFLDRFDLSLEIPLPPPGLLRQTGIKG
ESSAVVRERVIAAQARQYARQNRLNARLDNSGIRQFCSLNAEDAGWLEETLTRFGLSIRAWQRLLKVARTVADVEGCPGI
ERRHLQEALSYRAIDRLLLHLQKMLA

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=94513 NGK28_RS21925 WP_022649778.1 4577614..4579134(+) (comM) [Enterobacter hormaechei strain FUJ80066]
ATGTCACTGTCAGTTGTTTATACACGCGCGGCCATCGGCGTGAAGGCACCGCTTATTTCTGTAGAGGTTCATTTAAGTAA
TGGACTGCCCGGACTGACGCTGGTCGGCTTACCCGAAACGACCGTTAAGGAGGCCAGGGATCGCGTGCGCAGCGCAATTA
TTAATAGCGGTTATGCTTTTCCGGCGAAGAAGATCACCATCAACCTTGCGCCCGCCGATTTGCCAAAGGAGGGAGGGCGA
TATGATTTACCTATTGCTATAGCGCTTCTCGCGGCTTCTGAACAGCTCAATACGCCAGGGTTAAGCTCGTGCGAGTTCGT
GGGTGAATTAGCGCTTACAGGCGCGTTAAGAGGCGTTCCCGGAGCAATCTCGGGTGCGCTTGAAGCCATACGCGCGGGAA
GGCAAATCATTGTGGCCAATGAAAATGCCTCTGAAGTCAGCCTTATCGCCGAAAAGGGGTGTCTTGTCGCGGGACATTTG
CAGGAGGTTTGCGCCTGGCTGGAAGGCCGACACGAGCTTGCCGAGCCGCAGGAGAATGAGGAGGTAGTGGCAGATTCACC
CGATGACCTCAGCGATATTATGGGTCAGGAACAGGGTAAGCGGGCGTTAGAAATAACGGCCGCGGGTGGGCATAACCTGC
TACTGATAGGTCCGCCAGGCACGGGGAAAACGATGCTGGCGAGCAGGTTGAGCGGCCTGCTGCCCCCCCTCAATAATCAC
GAAGCGCTGGAAAGCGCCGCGATATTTAGCCTGGTCAGTTCAATATCGCTGCATAAACAGTGGCGCCGCCGTCCTTTCCG
CTCGCCACACCACAGTGCGTCTCTTACCGCGATGGTGGGCGGGGGCTCAATTCCCGGACCGGGTGAAATCTCACTGGCAC
ACAACGGCATTCTGTTCCTGGATGAATTGCCAGAGTTTGAACGCCGCGTGCTGGATGCGTTGCGGGAGCCGATTGAATCC
GGCGAAATCCATCTGTCTCGTACACGCGCCAAAATAAGCTACCCTGCGCAGTTTCAGCTGGTCGCCGCGATGAATCCTAG
CCCAACGGGCCATTATCAGGGCAACCATAACCGCTGTACGCCGGAGCAGACGCTGCGCTATCTGAGTAAGCTGTCCGGCC
CCTTCCTCGACCGTTTTGATTTATCTCTCGAGATCCCCCTGCCACCGCCCGGTCTGCTGAGGCAAACCGGTATCAAAGGC
GAAAGCTCAGCAGTGGTGCGAGAACGGGTTATTGCGGCTCAGGCGCGGCAGTATGCTCGTCAGAACAGGCTGAATGCCCG
GCTGGATAACAGCGGGATCCGACAGTTTTGCTCTCTCAACGCCGAAGATGCAGGCTGGCTGGAAGAGACACTGACGCGGT
TTGGGTTATCCATTCGCGCGTGGCAGCGTTTGTTGAAAGTTGCGCGCACGGTCGCTGACGTGGAGGGCTGCCCCGGAATA
GAGAGGCGACATTTGCAGGAGGCATTAAGCTACCGTGCGATCGATCGGCTGCTGTTGCATCTGCAAAAAATGCTGGCATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0HFG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.939

100

0.593
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio campbellii strain DS40M4

58.648

99.407

0.583
  comM Vibrio cholerae strain A1552

58.566

99.209

0.581
  comM Legionella pneumophila str. Paris

49.497

98.221

0.486
  comM Legionella pneumophila strain ERS1305867

49.497

98.221

0.486
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

43.393

100

0.435

Multiple sequence alignment