Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   V9W64_RS08805 Genome accession   NZ_CP146598
Coordinates   1888448..1890169 (+) Length   573 a.a.
NCBI ID   WP_274584864.1    Uniprot ID   A0A9X4E2R0
Organism   Neisseria leonii strain 51.81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1883448..1895169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W64_RS08790 (V9W64_08790) ilvD 1884574..1886436 (-) 1863 WP_274584862.1 dihydroxy-acid dehydratase -
  V9W64_RS08795 (V9W64_08795) asd 1886581..1887702 (-) 1122 WP_274584863.1 aspartate-semialdehyde dehydrogenase -
  V9W64_RS08805 (V9W64_08805) pilF 1888448..1890169 (+) 1722 WP_274584864.1 GspE/PulE family protein Machinery gene
  V9W64_RS08810 (V9W64_08810) - 1890176..1891387 (+) 1212 WP_274584865.1 type II secretion system F family protein -
  V9W64_RS08815 (V9W64_08815) - 1891387..1892208 (+) 822 WP_274584866.1 A24 family peptidase -
  V9W64_RS08820 (V9W64_08820) coaE 1892205..1892822 (+) 618 WP_274584867.1 dephospho-CoA kinase -
  V9W64_RS08825 (V9W64_08825) zapD 1892830..1893591 (+) 762 WP_274584868.1 cell division protein ZapD -
  V9W64_RS08830 (V9W64_08830) - 1893594..1893770 (+) 177 WP_274584869.1 DNA gyrase inhibitor YacG -
  V9W64_RS08835 (V9W64_08835) - 1893822..1894136 (+) 315 WP_274584870.1 hypothetical protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 61759.11 Da        Isoelectric Point: 6.2027

>NTDB_id=943270 V9W64_RS08805 WP_274584864.1 1888448..1890169(+) (pilF) [Neisseria leonii strain 51.81]
MNRAVRRQAVCFGSAEPENGLADGGGMASGALRILAGHFGWDGTVLRRCEEWARQGLLLPKVFEEGLCTPDEAAAVLAQA
VGRPYVDLAAFAPEDTAMTAEQMRRFGCVPLARRQGRLLAGVADPTDPALQRLAFVCAEPVDLAVVRYDLLVQRLAESGG
GTAGILAQMPQEEAGTAAAWAEEAGDGPVARFVQQVLEDAWAAGASDVHFEFYEQAARVRFRVDGVLREVVQPPHAVRRR
LVSRIKVMAGLDIAEQRLPQDGRLQTVLGGRTADLRISTLPTLFGEKVVVRILNGDAQAPDLARLGLEPCQYDLLLAEIR
RPYGMILVTGPTGSGKTLSLYACLNELNSERVNIAAVEDPAEINLAGINQVNVNEKQGLTFAEALRAFLRQDPDVLMVGE
IRDLETADIAVKAAQTGHLVFSTLHTNRAAASLTRLLNMGVAPFHIAASVNLVVAQRLVRKLCPHCREVWERPSENVLAA
AGFTLQDLAQDWPLYRPKGCAACRGKGYRGRIGVFELMPVSQAMRRAMMEGAGELDLAALAAREGMVDLRRAGLLKVMAG
ATTLAEVDAVVGR

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=943270 V9W64_RS08805 WP_274584864.1 1888448..1890169(+) (pilF) [Neisseria leonii strain 51.81]
ATGAATCGGGCAGTACGGCGGCAGGCCGTCTGTTTTGGATCGGCGGAGCCGGAAAACGGGTTGGCGGACGGAGGCGGTAT
GGCATCGGGGGCATTGCGGATTTTGGCCGGACATTTCGGTTGGGACGGAACGGTGTTGCGCCGTTGCGAAGAATGGGCGC
GGCAGGGGCTGCTGCTGCCCAAGGTGTTTGAAGAGGGTCTGTGTACGCCCGATGAGGCAGCGGCGGTGCTGGCGCAGGCA
GTCGGGCGGCCGTATGTGGATTTGGCGGCATTTGCGCCCGAAGATACGGCGATGACGGCGGAACAGATGCGCCGTTTCGG
CTGTGTGCCGCTGGCACGGCGGCAGGGGCGGCTGCTGGCGGGCGTGGCCGATCCGACGGATCCCGCGTTGCAGCGGTTGG
CTTTTGTCTGCGCCGAACCGGTCGATCTGGCGGTGGTGCGCTACGATCTGCTGGTGCAGCGGCTGGCAGAGTCGGGTGGC
GGTACGGCGGGGATTCTGGCGCAAATGCCGCAGGAGGAAGCGGGAACGGCCGCCGCTTGGGCGGAAGAAGCGGGCGACGG
GCCGGTGGCGCGTTTTGTGCAGCAGGTGTTGGAAGATGCGTGGGCGGCGGGTGCGTCGGACGTGCATTTCGAGTTTTACG
AGCAGGCGGCGCGGGTGCGTTTCCGCGTGGACGGTGTGCTGCGCGAAGTGGTGCAGCCGCCTCATGCGGTCAGACGGCGG
CTGGTGTCGCGGATTAAAGTGATGGCGGGGCTGGATATTGCCGAACAGCGGCTGCCGCAGGACGGGCGTTTGCAGACGGT
TTTGGGCGGGCGCACGGCGGATTTGCGCATCAGCACGCTGCCGACGCTTTTCGGCGAAAAAGTGGTGGTGCGGATTCTGA
ACGGCGATGCGCAGGCGCCGGATTTGGCGCGTTTGGGGCTGGAGCCGTGCCAATATGACTTGCTGCTGGCCGAAATCCGC
CGCCCTTACGGCATGATTCTGGTAACCGGGCCGACCGGTTCGGGCAAAACGCTGTCGCTGTATGCCTGTCTGAACGAATT
GAACAGCGAGCGGGTGAATATTGCGGCGGTGGAAGACCCGGCGGAAATCAATTTGGCGGGCATCAATCAGGTGAATGTGA
ACGAGAAGCAGGGGCTGACGTTTGCCGAGGCTCTGCGGGCGTTTCTGCGGCAGGATCCCGATGTGCTGATGGTGGGCGAA
ATCCGCGATTTGGAAACGGCCGATATTGCTGTCAAGGCGGCGCAGACGGGGCATTTGGTGTTTTCCACGCTGCATACCAA
CCGCGCGGCGGCCAGTCTGACGCGGCTGCTGAATATGGGGGTGGCGCCGTTCCATATTGCCGCTTCGGTGAATCTGGTGG
TGGCGCAGCGGCTGGTACGTAAGCTGTGTCCGCATTGCCGCGAAGTGTGGGAGCGGCCGTCTGAAAACGTGTTGGCGGCG
GCAGGGTTTACGCTGCAGGATTTGGCGCAGGATTGGCCGCTGTACCGTCCCAAAGGCTGCGCGGCCTGCCGAGGTAAGGG
TTATCGGGGGCGTATCGGCGTATTCGAGCTGATGCCGGTCAGTCAGGCAATGCGGCGGGCGATGATGGAGGGAGCGGGCG
AATTGGATTTGGCGGCATTGGCCGCACGGGAGGGTATGGTGGATTTGCGCCGTGCCGGATTGCTGAAAGTGATGGCGGGT
GCGACCACGCTGGCGGAAGTCGATGCGGTGGTGGGAAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.578

97.208

0.531
  pilB Vibrio cholerae strain A1552

45.216

93.019

0.421
  pilB Acinetobacter baumannii D1279779

44.693

93.717

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

43.831

94.764

0.415
  pilB Vibrio campbellii strain DS40M4

43.462

94.764

0.412
  pilB Legionella pneumophila strain ERS1305867

43.657

93.543

0.408
  pilB Acinetobacter baylyi ADP1

44.151

92.496

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.139

93.194

0.365
  pilF Thermus thermophilus HB27

39.057

92.496

0.361