Detailed information NTG0939691

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: V6D45_RS13020
• Genome accession: NZ_CP145722
• Coordinates: 2563532..2564149 (-)
• Length: 205 a.a.
• NCBI ID: WP_268393816.1
• Uniprot ID: A0A9Q4E6Z9
• Organism: Bacillus spizizenii strain kcgeb_S11
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2558532..2569149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6D45_RS12990 (V6D45_12995)	rpsT	2558833..2559099 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
V6D45_RS12995 (V6D45_13000)	holA	2559114..2560157 (-)	1044	WP_338442862.1	DNA polymerase III subunit delta	-
V6D45_RS13000 (V6D45_13005)	-	2560197..2560346 (-)	150	WP_338442863.1	hypothetical protein	-
V6D45_RS13005 (V6D45_13010)	-	2560387..2560521 (+)	135	WP_003229983.1	YqzM family protein	-
V6D45_RS13010 (V6D45_13015)	comEC	2560562..2562892 (-)	2331	WP_338442864.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
V6D45_RS13015 (V6D45_13020)	-	2562896..2563465 (-)	570	WP_014114472.1	ComE operon protein 2	-
V6D45_RS13020 (V6D45_13025)	comEA	2563532..2564149 (-)	618	WP_268393816.1	helix-hairpin-helix domain-containing protein	Machinery gene
V6D45_RS13025 (V6D45_13030)	comER	2564233..2565054 (+)	822	WP_338442865.1	late competence protein ComER	-
V6D45_RS13030 (V6D45_13035)	-	2565120..2565863 (-)	744	WP_268393818.1	class I SAM-dependent methyltransferase	-
V6D45_RS13035 (V6D45_13040)	rsfS	2565860..2566216 (-)	357	WP_003237112.1	ribosome silencing factor	-
V6D45_RS13040 (V6D45_13045)	yqeK	2566234..2566794 (-)	561	WP_338442866.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
V6D45_RS13045 (V6D45_13050)	-	2566784..2567353 (-)	570	WP_014114477.1	nicotinate-nucleotide adenylyltransferase	-
V6D45_RS13050 (V6D45_13055)	yhbY	2567365..2567655 (-)	291	WP_014114478.1	ribosome assembly RNA-binding protein YhbY	-
V6D45_RS13055 (V6D45_13060)	aroE	2567649..2568491 (-)	843	WP_338442867.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22001.84 Da
• Isoelectric Point: 4.8542

>NTDB_id=939691 V6D45_RS13020 WP_268393816.1 2563532..2564149(-) (comEA) [Bacillus spizizenii strain kcgeb_S11]
MNWLSQHKKAIILAASAAVFIAVIFFLASGKNKEPMKQTVSTETENTEVKQEAFKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAELLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTTEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=939691 V6D45_RS13020 WP_268393816.1 2563532..2564149(-) (comEA) [Bacillus spizizenii strain kcgeb_S11]
ATGAATTGGTTGAGCCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGGTTATTTTCTTCCT
GGCCAGTGGGAAAAACAAAGAGCCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAT
TCAAAGACGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCATCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCTCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGA
GCTGCTGCAGGACGGGACAGTGGTATACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGGAAGGGGGCGCTGGTAAACATCAATACAGCAACATTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAACTGAAGATATCACGAAGGT
TTCAGGAATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	89.756	100	0.898
comEA	Staphylococcus aureus MW2	36.866	100	0.39
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	37.264	100	0.385
comEA	Staphylococcus aureus N315	34.562	100	0.366