Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ORY85_RS00190 Genome accession   NZ_CP145606
Coordinates   39144..40787 (+) Length   547 a.a.
NCBI ID   WP_405030358.1    Uniprot ID   -
Organism   Neisseria leonii strain 3986     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34144..45787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORY85_RS00170 (ORY85_00170) - 34605..34832 (-) 228 WP_274570731.1 hypothetical protein -
  ORY85_RS00175 (ORY85_00175) ilvD 35263..37125 (-) 1863 WP_274570730.1 dihydroxy-acid dehydratase -
  ORY85_RS00180 (ORY85_00180) asd 37275..38396 (-) 1122 WP_274570729.1 aspartate-semialdehyde dehydrogenase -
  ORY85_RS00190 (ORY85_00190) pilF 39144..40787 (+) 1644 WP_405030358.1 GspE/PulE family protein Machinery gene
  ORY85_RS00195 (ORY85_00195) - 40809..42002 (+) 1194 WP_274570727.1 type II secretion system F family protein -
  ORY85_RS00200 (ORY85_00200) - 42002..42823 (+) 822 WP_274570726.1 A24 family peptidase -
  ORY85_RS00205 (ORY85_00205) coaE 42820..43437 (+) 618 WP_274570725.1 dephospho-CoA kinase -
  ORY85_RS00210 (ORY85_00210) zapD 43445..44206 (+) 762 WP_274570724.1 cell division protein ZapD -
  ORY85_RS00215 (ORY85_00215) - 44209..44385 (+) 177 WP_274570723.1 DNA gyrase inhibitor YacG -
  ORY85_RS00220 (ORY85_00220) - 44437..44751 (+) 315 WP_274570722.1 hypothetical protein -

Sequence

Protein


Download         Length: 547 a.a.        Molecular weight: 59168.28 Da        Isoelectric Point: 6.1989

>NTDB_id=938995 ORY85_RS00190 WP_405030358.1 39144..40787(+) (pilF) [Neisseria leonii strain 3986]
MASGALRILAGHFGWDGTVLRRCEEWARQGLLLPKLFEEGLCTPDEVAAVLAQAVGRPYVDLAAFAPEDTAMTAEQMRRF
GCVPLARRQGRLLAGVADPTDPVLQRLAFVCAEPVDLAVVRYDLLVQRLAESGGGTAGILAQMPQEEAGTAAAWAEEAGD
GPVARFVQQVLEDAWAAGASDVHFEFYEQAARVRFRVDGVLREVVQPPHAVRRRLVSRIKVMAGLDIAEQRLPQDGRLQT
VLGGRTADLRISTLPTLFGEKVVVRILNGDAQAPDLARLGLEPCQYDLLLAEIRRPYGMILVTGPTGSGKTLSLYACLNE
LNSERVNIAAVEDPAEINLAGINQVNVNEKQGLTFAEALRAFLRQDPDVLMVGEIRDLETADIAVKAAQTGHLVFSTLHT
NRAAASLTRLLNMGVAPFHIAASVNLVVAQRLVRKLCPHCREVWERPSENVLAAAGFTPQDLAQDWPLYRPKGCAACRGK
GYRGRIGIFELMPVSQAMRRAMMDGAGELDLAALAAREGMVDLRRAGLLKVMAGATTLAEVDAVVGR

Nucleotide


Download         Length: 1644 bp        

>NTDB_id=938995 ORY85_RS00190 WP_405030358.1 39144..40787(+) (pilF) [Neisseria leonii strain 3986]
ATGGCATCGGGGGCATTGCGGATTTTGGCCGGACATTTCGGTTGGGACGGAACGGTGTTGCGCCGTTGCGAAGAGTGGGC
GCGGCAGGGGCTGCTGCTGCCCAAGCTGTTTGAAGAGGGTTTGTGCACGCCCGATGAGGTGGCGGCGGTGCTGGCGCAGG
CGGTCGGGCGGCCGTATGTGGATTTGGCGGCATTTGCGCCCGAAGATACGGCGATGACGGCGGAACAGATGCGCCGTTTC
GGCTGTGTGCCGCTGGCACGGCGGCAGGGGCGGCTGCTGGCAGGTGTGGCCGATCCGACGGATCCCGTGTTGCAGCGGTT
GGCTTTTGTCTGCGCCGAACCGGTCGATCTGGCGGTGGTGCGCTACGATCTGCTGGTGCAGCGGCTGGCAGAGTCGGGTG
GCGGTACAGCGGGGATTCTGGCGCAAATGCCGCAGGAGGAAGCGGGAACGGCCGCCGCTTGGGCGGAAGAAGCGGGCGAC
GGGCCGGTGGCGCGTTTCGTGCAGCAGGTGTTGGAAGATGCGTGGGCGGCGGGTGCGTCGGACGTGCATTTCGAGTTTTA
CGAGCAGGCGGCGCGGGTGCGTTTCCGCGTGGACGGTGTGTTGCGTGAAGTGGTGCAGCCGCCTCATGCGGTCAGACGGC
GGCTGGTGTCGCGGATTAAGGTAATGGCGGGGCTGGACATTGCCGAACAGCGGCTGCCGCAGGACGGGCGTTTGCAGACG
GTTTTGGGCGGGCGCACGGCGGATTTGCGCATCAGCACGCTGCCGACGCTTTTCGGCGAAAAAGTGGTGGTGCGGATTCT
GAACGGCGATGCGCAGGCGCCGGATTTGGCGCGCTTGGGGCTGGAGCCGTGCCAATATGACTTGCTGCTGGCCGAAATCC
GCCGCCCTTACGGCATGATTCTGGTCACCGGGCCGACCGGTTCGGGCAAAACGCTGTCGCTGTATGCCTGTCTGAACGAA
TTGAACAGCGAGCGGGTAAATATTGCGGCGGTGGAAGACCCGGCGGAAATCAATCTGGCGGGCATCAATCAGGTAAATGT
GAACGAGAAGCAGGGGCTGACGTTTGCCGAGGCTCTGCGGGCGTTTCTGCGCCAGGATCCCGATGTGCTGATGGTGGGCG
AAATCCGCGATTTGGAAACGGCCGATATTGCCGTCAAGGCGGCGCAGACGGGGCATTTGGTATTTTCCACGCTGCATACC
AACCGCGCGGCGGCCAGTCTGACGCGGCTGCTGAATATGGGCGTGGCGCCGTTTCATATTGCCGCTTCGGTGAATCTGGT
TGTGGCACAGCGGCTGGTACGCAAGCTGTGTCCGCATTGTCGTGAAGTGTGGGAGCGGCCGTCTGAAAATGTGTTGGCGG
CGGCAGGGTTTACGCCGCAGGATTTGGCGCAGGATTGGCCGCTGTACCGTCCCAAAGGTTGCGCGGCCTGCCGCGGCAAG
GGTTATCGGGGGCGTATCGGCATATTCGAGCTGATGCCGGTCAGTCAGGCGATGCGGCGGGCGATGATGGACGGTGCGGG
CGAATTGGATTTGGCGGCATTGGCCGCACGGGAGGGTATGGTGGATTTGCGCCGTGCCGGATTGCTGAAAGTGATGGCGG
GTGCGACCACGCTGGCGGAAGTCGATGCCGTGGTGGGGAGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(169-435)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.578

100

0.556
  pilB Vibrio cholerae strain A1552

45.421

97.806

0.444
  pilB Acinetobacter baumannii D1279779

44.879

98.172

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

43.693

100

0.437
  pilB Vibrio campbellii strain DS40M4

43.831

99.269

0.435
  pilB Acinetobacter baylyi ADP1

44.34

96.892

0.43
  pilB Legionella pneumophila strain ERS1305867

43.47

97.989

0.426
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.326

97.623

0.384
  pilF Thermus thermophilus HB27

39.434

96.892

0.382