Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   ACEY2B_RS02095 Genome accession   NZ_CP169315
Coordinates   404835..406718 (+) Length   627 a.a.
NCBI ID   WP_015427040.1    Uniprot ID   -
Organism   Lactococcus lactis strain IFLLLBEK1     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 399835..411718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY2B_RS02065 (ACEY2B_02065) - 400949..401191 (+) 243 WP_003131476.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  ACEY2B_RS02070 (ACEY2B_02070) amaP 401219..401779 (+) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  ACEY2B_RS02075 (ACEY2B_02075) - 401790..401978 (+) 189 WP_012898445.1 DUF2273 domain-containing protein -
  ACEY2B_RS02080 (ACEY2B_02080) - 401999..402505 (+) 507 WP_374788169.1 Asp23/Gls24 family envelope stress response protein -
  ACEY2B_RS02085 (ACEY2B_02085) rsmB 402704..403978 (+) 1275 WP_015427042.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACEY2B_RS02090 (ACEY2B_02090) - 404059..404835 (+) 777 WP_015427041.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACEY2B_RS02095 (ACEY2B_02095) pknB 404835..406718 (+) 1884 WP_015427040.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ACEY2B_RS02100 (ACEY2B_02100) rpsO 406901..407170 (+) 270 WP_015427039.1 30S ribosomal protein S15 -
  ACEY2B_RS02105 (ACEY2B_02105) proC 407248..408036 (-) 789 WP_015427038.1 pyrroline-5-carboxylate reductase -
  ACEY2B_RS02110 (ACEY2B_02110) glmU 408264..409640 (+) 1377 WP_015427037.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  ACEY2B_RS02115 (ACEY2B_02115) - 409745..410329 (+) 585 WP_015427036.1 NUDIX hydrolase -
  ACEY2B_RS02120 (ACEY2B_02120) macP 410322..410600 (+) 279 WP_015427035.1 cell wall synthase accessory phosphoprotein MacP -
  ACEY2B_RS02125 (ACEY2B_02125) - 410632..411312 (+) 681 WP_015427034.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67816.83 Da        Isoelectric Point: 4.7347

>NTDB_id=938989 ACEY2B_RS02095 WP_015427040.1 404835..406718(+) (pknB) [Lactococcus lactis strain IFLLLBEK1]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLVILLLVVGGATLAWVVSTPTNVKIPNVTNSTLSQAKSKIKDAKLKVGTVHKQQSSTI
AEGNVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTSQQADGYVYSVYPTVGA
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTPETSSSTTSSTSSSTTSQPSTDNNNSNKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=938989 ACEY2B_RS02095 WP_015427040.1 404835..406718(+) (pknB) [Lactococcus lactis strain IFLLLBEK1]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGCGGCGGAATGGCGAATGTTTA
TCAAGGTGAGGATACTTTTCTTGGTGACCGCTTGGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAATGATGACATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTTATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACGGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAGGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCAGTTCATTATTTATCACCAGAGCAAGCGCGTGGCTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAGCTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTCGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAGGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTGTTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CACTTAGCCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTAGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAATGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCTTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAGTCAACAAGCTGACGGTTATGTATATTCTGTTTATCCGACTGTTGGAGCG
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACAACGACGCCTGAAACTTCGTCAAGCACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCGACAGATAACA
ACAATAGCAACAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(434-501)

Protein kinase domain (Pkinase)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

49.081

86.762

0.426
  stkP/pknB Streptococcus salivarius strain HSISS4

51.252

82.775

0.424
  stkP Streptococcus pneumoniae TIGR4

48.024

80.702

0.388
  stkP Streptococcus pneumoniae D39

47.826

80.702

0.386
  stkP Streptococcus pneumoniae R6

47.826

80.702

0.386
  stkP Streptococcus pneumoniae Rx1

47.628

80.702

0.384