Detailed information NTG0937428

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: V5F86_RS13025
• Genome accession: NZ_CP145137
• Coordinates: 2563805..2564422 (-)
• Length: 205 a.a.
• NCBI ID: WP_268393816.1
• Uniprot ID: A0A9Q4E6Z9
• Organism: Bacillus spizizenii strain kcgeb_sc
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2558805..2569422

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5F86_RS12995 (V5F86_13000)	rpsT	2559106..2559372 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
V5F86_RS13000 (V5F86_13005)	holA	2559387..2560430 (-)	1044	WP_338442862.1	DNA polymerase III subunit delta	-
V5F86_RS13005 (V5F86_13010)	-	2560470..2560619 (-)	150	WP_338442863.1	hypothetical protein	-
V5F86_RS13010 (V5F86_13015)	-	2560660..2560794 (+)	135	WP_003229983.1	YqzM family protein	-
V5F86_RS13015 (V5F86_13020)	comEC	2560835..2563165 (-)	2331	WP_338442864.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
V5F86_RS13020 (V5F86_13025)	-	2563169..2563738 (-)	570	WP_014114472.1	ComE operon protein 2	-
V5F86_RS13025 (V5F86_13030)	comEA	2563805..2564422 (-)	618	WP_268393816.1	helix-hairpin-helix domain-containing protein	Machinery gene
V5F86_RS13030 (V5F86_13035)	comER	2564506..2565327 (+)	822	WP_338442865.1	late competence protein ComER	-
V5F86_RS13035 (V5F86_13040)	-	2565393..2566136 (-)	744	WP_268393818.1	class I SAM-dependent methyltransferase	-
V5F86_RS13040 (V5F86_13045)	rsfS	2566133..2566489 (-)	357	WP_003237112.1	ribosome silencing factor	-
V5F86_RS13045 (V5F86_13050)	yqeK	2566507..2567067 (-)	561	WP_338442866.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
V5F86_RS13050 (V5F86_13055)	-	2567057..2567626 (-)	570	WP_014114477.1	nicotinate-nucleotide adenylyltransferase	-
V5F86_RS13055 (V5F86_13060)	yhbY	2567638..2567928 (-)	291	WP_014114478.1	ribosome assembly RNA-binding protein YhbY	-
V5F86_RS13060 (V5F86_13065)	aroE	2567922..2568764 (-)	843	WP_338442867.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22001.84 Da
• Isoelectric Point: 4.8542

>NTDB_id=937428 V5F86_RS13025 WP_268393816.1 2563805..2564422(-) (comEA) [Bacillus spizizenii strain kcgeb_sc]
MNWLSQHKKAIILAASAAVFIAVIFFLASGKNKEPMKQTVSTETENTEVKQEAFKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAELLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTTEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=937428 V5F86_RS13025 WP_268393816.1 2563805..2564422(-) (comEA) [Bacillus spizizenii strain kcgeb_sc]
ATGAATTGGTTGAGCCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGGTTATTTTCTTCCT
GGCCAGTGGGAAAAACAAAGAGCCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAT
TCAAAGACGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCATCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCTCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGA
GCTGCTGCAGGACGGGACAGTGGTATACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGGAAGGGGGCGCTGGTAAACATCAATACAGCAACATTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAACTGAAGATATCACGAAGGT
TTCAGGAATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	89.756	100	0.898
comEA	Staphylococcus aureus MW2	36.866	100	0.39
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	37.264	100	0.385
comEA	Staphylococcus aureus N315	34.562	100	0.366