Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACEPM4_RS14820 Genome accession   NZ_CP168733
Coordinates   3158982..3161120 (-) Length   712 a.a.
NCBI ID   WP_276145546.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN5935     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3153982..3166120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM4_RS14795 (ACEPM4_14795) - 3154166..3154891 (-) 726 WP_064478099.1 transposase -
  ACEPM4_RS14800 (ACEPM4_14800) - 3155158..3155832 (+) 675 WP_011002872.1 OmpW family protein -
  ACEPM4_RS14805 (ACEPM4_14805) - 3155982..3157145 (-) 1164 WP_064478100.1 deoxyguanosinetriphosphate triphosphohydrolase -
  ACEPM4_RS14810 (ACEPM4_14810) aroB 3157165..3158271 (-) 1107 WP_405124950.1 3-dehydroquinate synthase -
  ACEPM4_RS14815 (ACEPM4_14815) - 3158268..3158840 (-) 573 WP_011002875.1 shikimate kinase -
  ACEPM4_RS14820 (ACEPM4_14820) pilQ 3158982..3161120 (-) 2139 WP_276145546.1 temperature dependent type IV pilus secretin PilQ Machinery gene
  ACEPM4_RS14825 (ACEPM4_14825) - 3161123..3161668 (-) 546 WP_011002877.1 pilus assembly protein PilP -
  ACEPM4_RS14830 (ACEPM4_14830) - 3161665..3162324 (-) 660 WP_020832821.1 type 4a pilus biogenesis protein PilO -
  ACEPM4_RS14835 (ACEPM4_14835) - 3162327..3162959 (-) 633 WP_011002879.1 PilN domain-containing protein -
  ACEPM4_RS14840 (ACEPM4_14840) pilM 3162973..3164043 (-) 1071 WP_011002880.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76349.44 Da        Isoelectric Point: 9.8259

>NTDB_id=936799 ACEPM4_RS14820 WP_276145546.1 3158982..3161120(-) (pilQ) [Ralstonia pseudosolanacearum strain RUN5935]
MRRVKGGLVQAVRVARGGAVAWLSLCMLMVGGQALAQQAITAPASNAVERVEQASTGESTVVTVTLKDAPAQKPVEFSTQ
QPARIAIDFFGTAFAQGRANYQYGGKLLRAASVVQIGDRTRVVLDLARQSQYKSEIRGNQYVLTLGAAPTASATPVPTFA
APAPTAGVERPSVRNIDFRRGEELAGRVVVDLSTSNSAINIAQQGQNLVVDFAGATLPQSLRRRFDVSDFGTPVQAMRAT
DNGTGARLVIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERMSLNFQNIDIRSLLQVFADFTNLNIVTSD
SVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRNELATKEKAELESQQQVTELEPLRSQVFRLNYQRADDV
RNMLLGTGGTAGGGAAASRILSKRGSLTSDARTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEADDTFSRNLG
AKLGFAAKTNGAGYGNTYSNVVSPVTKDATWDNSAAVSLPANGINGVNAASVAVSLFNAGAGRFLALELSALEADGRGKI
ISSPRVVTADNIKALIEQGTEVPYQQATSSGATSVSFKKANLKLEVTPKITPDGNVFLDVDVNKDSLGTQTTNGFAINTK
HVQTQVLVENGGTVVIGGIYTQNERTDVNKVPLLGDIPVLGNLFKSTAKTNDRTELLVFLTPRVLSDQLSLK

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=936799 ACEPM4_RS14820 WP_276145546.1 3158982..3161120(-) (pilQ) [Ralstonia pseudosolanacearum strain RUN5935]
ATGCGTCGCGTGAAGGGCGGCCTGGTGCAGGCCGTTCGAGTGGCCCGGGGGGGCGCAGTGGCATGGCTTTCGCTGTGCAT
GCTGATGGTGGGTGGACAGGCGTTGGCCCAGCAGGCAATCACGGCGCCGGCAAGCAATGCCGTCGAACGCGTGGAACAAG
CCTCGACAGGCGAATCCACCGTGGTGACGGTGACGCTGAAGGACGCGCCCGCCCAGAAGCCGGTGGAATTCAGCACGCAG
CAGCCTGCGCGCATCGCCATCGACTTCTTCGGCACGGCATTCGCCCAGGGGCGTGCCAACTATCAATATGGCGGCAAGCT
GCTGCGCGCGGCCAGCGTGGTGCAGATCGGTGACCGCACCCGCGTCGTGCTGGATCTGGCGCGCCAGTCGCAGTACAAGT
CCGAGATCCGGGGCAACCAGTACGTGCTGACGCTGGGCGCCGCGCCGACGGCGTCGGCCACCCCGGTGCCGACCTTCGCC
GCGCCCGCGCCGACGGCCGGTGTGGAGCGGCCGTCCGTGCGCAACATCGACTTCCGCCGTGGCGAAGAACTGGCCGGCCG
CGTGGTGGTCGACCTGTCGACGTCCAACTCCGCGATCAACATCGCTCAGCAGGGGCAAAACCTGGTGGTCGATTTCGCCG
GTGCCACGCTGCCGCAATCGCTGCGTCGCCGCTTTGACGTGAGCGACTTCGGCACGCCGGTGCAGGCCATGCGCGCCACC
GACAACGGCACGGGCGCTCGCCTGGTCATCGAGCCGCGCGGCAACTGGCAGTACAGCTCGTACCAGACCGACACGCAGTT
TGTGGTGGAAGTCCGCCCGACCAAGGAGGACCCGAACAAGCTGATCTCCGGTCCGGGCTATCGCGGGGAGCGCATGTCGC
TGAACTTCCAGAACATCGACATTCGTTCGCTGCTGCAGGTCTTTGCCGACTTCACCAACCTGAACATCGTCACCAGCGAC
AGCGTGACGGGGACCCTGTCGCTGCGTCTGAAGGACGTGCCGTGGGATCAGGCGCTGCAGATCGTGCTGGATTCCAAGGG
CCTGGCCTCGCGCCGCAACGGCAACGTGCTGTGGGTGGCGCCGCGCAACGAACTGGCGACCAAGGAGAAGGCTGAGCTGG
AGTCGCAGCAACAGGTGACCGAGCTGGAGCCGCTGCGCAGCCAGGTGTTCCGTCTGAATTACCAGCGTGCTGACGACGTG
CGCAACATGCTGCTGGGTACCGGAGGAACGGCGGGCGGTGGTGCTGCGGCGTCGCGCATCTTGTCCAAGCGTGGCTCGCT
GACGTCGGATGCCCGTACCAACCAGCTGTTCGTCTCGGATATCCCGAGCAAGCTGGAGGAGGTGCAGGCCTTCCTGCTGA
AGATCGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGATGACACGTTCAGCCGCAATCTTGGG
GCAAAGCTGGGCTTCGCGGCCAAGACCAATGGTGCCGGGTATGGCAACACCTACAGCAACGTGGTGTCGCCGGTCACGAA
AGACGCCACTTGGGATAACAGCGCGGCCGTCAGCCTGCCCGCCAATGGCATCAACGGTGTGAACGCCGCCAGTGTGGCAG
TGAGCTTGTTCAACGCCGGTGCCGGCCGTTTCCTGGCGCTCGAACTGTCGGCGCTGGAAGCCGATGGCCGCGGCAAGATC
ATCTCCAGCCCGCGTGTGGTCACCGCTGACAACATCAAGGCGCTGATCGAGCAGGGTACCGAAGTGCCTTACCAGCAGGC
GACCTCGTCGGGTGCGACGTCGGTTTCGTTCAAAAAGGCCAACCTGAAATTGGAGGTGACGCCGAAGATTACGCCGGACG
GCAACGTGTTCCTGGACGTGGACGTCAACAAGGACAGCCTGGGCACGCAGACCACCAACGGCTTCGCCATCAACACCAAG
CATGTGCAGACCCAGGTGTTGGTCGAGAACGGCGGTACGGTTGTGATTGGCGGCATCTACACGCAGAACGAACGGACCGA
TGTGAACAAGGTCCCGCTGCTGGGTGACATCCCGGTGCTGGGCAACCTGTTCAAGAGCACGGCCAAGACCAACGACCGGA
CGGAACTGCTGGTGTTCCTGACGCCGCGCGTGCTGTCCGACCAGCTGTCGCTGAAGTAA

Domains

Predicted by InterProScan.

AMIN domain (AMIN)

(61-143)

AMIN domain (AMIN)

(195-270)

Secretin and TonB N terminus short domain (STN)

(316-361)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-461)

Bacterial type II and III secretion system protein (Secretin)

(551-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

41.467

93.82

0.389
  pilQ Neisseria gonorrhoeae strain FA1090

36.401

100

0.372
  comQ/pilQ Acinetobacter baylyi ADP1

38.15

97.191

0.371
  pilQ Legionella pneumophila strain Lp02

39.269

92.275

0.362
  pilQ Legionella pneumophila strain ERS1305867

39.269

92.275

0.362