Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACEPM0_RS03455 Genome accession   NZ_CP168731
Coordinates   708430..708870 (+) Length   146 a.a.
NCBI ID   WP_197345369.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN6285     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703430..713870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM0_RS03430 (ACEPM0_03430) - 703994..704272 (-) 279 WP_011002595.1 hypothetical protein -
  ACEPM0_RS03435 (ACEPM0_03435) proC 704294..705118 (-) 825 WP_011002594.1 pyrroline-5-carboxylate reductase -
  ACEPM0_RS03440 (ACEPM0_03440) - 705153..705875 (-) 723 WP_011002593.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPM0_RS03445 (ACEPM0_03445) pilT 706000..707043 (+) 1044 WP_011002592.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPM0_RS03450 (ACEPM0_03450) pilU 707096..708235 (+) 1140 WP_011002591.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPM0_RS03455 (ACEPM0_03455) pilE 708430..708870 (+) 441 WP_197345369.1 type IV pilin protein Machinery gene
  ACEPM0_RS03460 (ACEPM0_03460) - 708874..709362 (+) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  ACEPM0_RS03465 (ACEPM0_03465) pilV 709359..709949 (+) 591 WP_011002588.1 type IV pilus modification protein PilV -
  ACEPM0_RS03470 (ACEPM0_03470) - 709946..710974 (+) 1029 WP_028853589.1 PilW family protein -
  ACEPM0_RS03475 (ACEPM0_03475) - 710978..711514 (+) 537 WP_011002586.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15231.23 Da        Isoelectric Point: 8.6699

>NTDB_id=936699 ACEPM0_RS03455 WP_197345369.1 708430..708870(+) (pilE) [Ralstonia pseudosolanacearum strain RUN6285]
MASKLACQRARSARGFTLIELMIVAAIVAILAVLAYPSYVQYVVRSNRAAAESFMQEVAAAQERFLLDNRAYAPDLATLQ
YASSVPASVASNYQFSFTAVTTAPPAYTLNATPTGSQSSSDAACGTLGLSNTGSKTASGGASNCWK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=936699 ACEPM0_RS03455 WP_197345369.1 708430..708870(+) (pilE) [Ralstonia pseudosolanacearum strain RUN6285]
GTGGCAAGCAAGCTTGCATGCCAGCGCGCGCGGTCAGCGCGGGGCTTCACGCTGATCGAGCTCATGATCGTGGCGGCGAT
TGTCGCCATTCTCGCGGTTCTGGCGTATCCCTCTTACGTTCAGTACGTCGTCCGCTCCAACCGGGCGGCGGCGGAATCGT
TCATGCAGGAAGTGGCTGCGGCCCAAGAGCGCTTCCTGCTCGATAACCGGGCCTATGCCCCGGACCTGGCGACGCTGCAA
TATGCCAGCAGTGTTCCGGCCAGCGTCGCCTCCAACTACCAGTTCAGCTTCACTGCCGTCACGACGGCTCCGCCGGCGTA
CACGCTGAATGCAACGCCGACGGGCAGCCAGTCCTCCAGCGACGCCGCGTGCGGCACGCTCGGCCTGTCGAACACCGGCA
GCAAGACGGCCTCCGGCGGTGCCTCCAACTGCTGGAAATAG

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(37-127)

Prokaryotic N-terminal methylation motif (N_methyl)

(11-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

39.552

91.781

0.363
  comF Acinetobacter baylyi ADP1

39.259

92.466

0.363