Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   V5F97_RS06460 Genome accession   NZ_CP145057
Coordinates   1238607..1239098 (+) Length   163 a.a.
NCBI ID   WP_353114437.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain WHO_alpha_2024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1233607..1244098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F97_RS06430 (V5F97_06460) - 1233984..1234739 (+) 756 WP_003702903.1 formylglycine-generating enzyme family protein -
  V5F97_RS06435 (V5F97_06465) - 1234982..1235365 (+) 384 Protein_1259 pilin -
  V5F97_RS06440 (V5F97_06470) lpxC 1236093..1237016 (+) 924 WP_003687007.1 UDP-3-O-acyl-N-acetylglucosamine deacetylase -
  V5F97_RS06445 (V5F97_06475) pilE 1237123..1237551 (+) 429 WP_050169685.1 pilin Machinery gene
  V5F97_RS06450 (V5F97_06480) - 1237674..1237792 (+) 119 Protein_1262 pilin -
  V5F97_RS06455 (V5F97_06485) - 1238095..1238238 (+) 144 WP_003691943.1 hypothetical protein -
  V5F97_RS06460 (V5F97_06490) pilE 1238607..1239098 (+) 492 WP_353114437.1 pilin Machinery gene
  V5F97_RS06465 (V5F97_06495) - 1239640..1240473 (+) 834 WP_353114439.1 opacity family porin -
  V5F97_RS06470 (V5F97_06500) - 1240447..1240656 (-) 210 WP_050169701.1 hypothetical protein -
  V5F97_RS06475 (V5F97_06505) - 1240674..1241231 (-) 558 WP_003691591.1 nitroreductase family protein -
  V5F97_RS06480 (V5F97_06510) - 1241306..1242940 (-) 1635 WP_003689187.1 phosphoethanolamine transferase -
  V5F97_RS06485 (V5F97_06515) - 1243003..1243341 (-) 339 WP_003689188.1 hypothetical protein -

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 17605.03 Da        Isoelectric Point: 8.4464

>NTDB_id=936612 V5F97_RS06460 WP_353114437.1 1238607..1239098(+) (pilE) [Neisseria gonorrhoeae strain WHO_alpha_2024]
MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAILLAEGQKSAVTEYYLNNGIWPKDNTSAGVASSSSIK
GKYVKEVEVKNGVVTAQMNPSGVNNEIKDKKLSLWAKRENGSVKWFCGQPVTRNGDTVAAGNDNKKIDTKHLPSTCRDES
TAE

Nucleotide


Download         Length: 492 bp        

>NTDB_id=936612 V5F97_RS06460 WP_353114437.1 1238607..1239098(+) (pilE) [Neisseria gonorrhoeae strain WHO_alpha_2024]
ATGAATACCCTTCAAAAAGGCTTTACCCTTATCGAGCTGATGATTGTGATCGCCATCGTCGGCATTTTGGCGGCAGTCGC
CCTTCCCGCCTACCAAGACTACACCGCCCGCGCGCAAGTTTCCGAAGCCATCCTTTTGGCCGAAGGTCAAAAATCAGCCG
TTACCGAGTATTACCTGAATAACGGCATATGGCCGAAAGACAACACTTCTGCCGGCGTGGCATCTTCTTCATCAATCAAA
GGCAAATATGTTAAAGAGGTTGAAGTTAAAAACGGCGTCGTCACCGCCCAAATGAATCCAAGCGGCGTAAACAATGAAAT
CAAAGACAAAAAACTCTCCCTGTGGGCCAAGCGTGAAAACGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTACGCGCA
ACGGCGACACCGTTGCCGCCGGCAACGACAACAAAAAAATCGACACCAAGCACCTGCCGTCAACCTGCCGCGATGAATCA
ACTGCCGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-157)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

84.848

100

0.859
  pilE Neisseria gonorrhoeae MS11

84.663

100

0.847
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.263

100

0.411
  comP Acinetobacter baylyi ADP1

37.805

100

0.38
  pilA/pilA1 Eikenella corrodens VA1

36.257

100

0.38
  pilA2 Legionella pneumophila strain ERS1305867

39.355

95.092

0.374
  pilA2 Legionella pneumophila str. Paris

38.71

95.092

0.368