Detailed information NTG0934310

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V4Q78_RS15265
• Genome accession: NZ_CP144563
• Coordinates: 3107986..3109104 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB5075 isolate grey variant
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3102986..3114104

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4Q78_RS15255 (V4Q78_15255)	-	3106102..3106794 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
V4Q78_RS15260 (V4Q78_15260)	pilT	3106921..3107958 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
V4Q78_RS15265 (V4Q78_15265)	pilU	3107986..3109104 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V4Q78_RS15270 (V4Q78_15270)	fur	3109174..3109611 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
V4Q78_RS15275 (V4Q78_15275)	bamE	3109723..3110121 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
V4Q78_RS15280 (V4Q78_15280)	-	3110176..3110499 (-)	324	WP_000442585.1	RnfH family protein	-
V4Q78_RS15285 (V4Q78_15285)	-	3110499..3111566 (-)	1068	WP_000092403.1	hypothetical protein	-
V4Q78_RS15290 (V4Q78_15290)	-	3111719..3112174 (-)	456	WP_000782976.1	bacteriohemerythrin	-
V4Q78_RS15295 (V4Q78_15295)	-	3112427..3113275 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=934310 V4Q78_RS15265 WP_000347036.1 3107986..3109104(+) (pilU) [Acinetobacter baumannii strain AB5075 isolate grey variant]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=934310 V4Q78_RS15265 WP_000347036.1 3107986..3109104(+) (pilU) [Acinetobacter baumannii strain AB5075 isolate grey variant]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392