Detailed information NTG0931815

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2198_RS01755
• Genome accession: NZ_CP143900
• Coordinates: 356937..358085 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PA19_NI3_44.22_ST207
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 351937..363085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2198_RS01735 (V2198_01735)	-	352674..353858 (-)	1185	WP_023092310.1	cystathionine gamma-synthase	-
V2198_RS01740 (V2198_01740)	-	353855..355228 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
V2198_RS01745 (V2198_01745)	-	355471..355875 (-)	405	WP_003084560.1	NINE protein	-
V2198_RS01750 (V2198_01750)	czcD	356030..356929 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V2198_RS01755 (V2198_01755)	pilU	356937..358085 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
V2198_RS01760 (V2198_01760)	pilT	358263..359297 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2198_RS01765 (V2198_01765)	-	359512..360204 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2198_RS01770 (V2198_01770)	proC	360216..361037 (+)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
V2198_RS01775 (V2198_01775)	-	361048..361641 (+)	594	WP_003084544.1	YggT family protein	-
V2198_RS01780 (V2198_01780)	-	361638..361934 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=931815 V2198_RS01755 WP_003110016.1 356937..358085(-) (pilU) [Pseudomonas aeruginosa strain PA19_NI3_44.22_ST207]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931815 V2198_RS01755 WP_003110016.1 356937..358085(-) (pilU) [Pseudomonas aeruginosa strain PA19_NI3_44.22_ST207]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382