Detailed information NTG0930690

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: ACB343_RS00660
• Genome accession: NZ_CP167012
• Coordinates: 102216..103250 (+)
• Length: 344 a.a.
• NCBI ID: WP_227868694.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain Isolate 17
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 97216..108250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACB343_RS00650 (ACB343_00650)	-	100884..101249 (+)	366	WP_002986560.1	DUF1033 family protein	-
ACB343_RS00655 (ACB343_00655)	comGA	101342..102280 (+)	939	WP_011284420.1	competence type IV pilus ATPase ComGA	Machinery gene
ACB343_RS00660 (ACB343_00660)	comGB	102216..103250 (+)	1035	WP_227868694.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACB343_RS00665 (ACB343_00665)	comGC	103252..103578 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
ACB343_RS00670 (ACB343_00670)	comGD	103553..103981 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACB343_RS00675 (ACB343_00675)	comGE	103938..104222 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACB343_RS00680 (ACB343_00680)	comGF	104215..104649 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACB343_RS00685 (ACB343_00685)	comGG	104633..104959 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
ACB343_RS00690 (ACB343_00690)	comGH	105057..106010 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
ACB343_RS00695 (ACB343_00695)	-	106069..107265 (+)	1197	WP_011284424.1	acetate kinase	-
ACB343_RS00700 (ACB343_00700)	-	107452..107760 (+)	309	WP_011284425.1	hypothetical protein	-

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 39734.19 Da
• Isoelectric Point: 9.6101

>NTDB_id=930690 ACB343_RS00660 WP_227868694.1 102216..103250(+) (comGB) [Streptococcus pyogenes strain Isolate 17]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQDLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide

• Length: 1035 bp

>NTDB_id=930690 ACB343_RS00660 WP_227868694.1 102216..103250(+) (comGB) [Streptococcus pyogenes strain Isolate 17]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGACTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterProScan.

• Type II secretion system (T2SS), protein F (T2SSF) (28-147)

• Type II secretion system (T2SS), protein F (T2SSF) (215-335)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Streptococcus mutans UA140	53.982	98.547	0.532
comGB	Streptococcus mutans UA159	53.982	98.547	0.532
comGB	Streptococcus gordonii str. Challis substr. CH1	50.588	98.837	0.5
comGB/cglB	Streptococcus mitis NCTC 12261	47.619	97.674	0.465
comGB/cglB	Streptococcus mitis SK321	47.748	96.802	0.462
comGB/cglB	Streptococcus pneumoniae Rx1	45.646	96.802	0.442
comGB/cglB	Streptococcus pneumoniae D39	45.646	96.802	0.442
comGB/cglB	Streptococcus pneumoniae R6	45.646	96.802	0.442
comGB/cglB	Streptococcus pneumoniae TIGR4	45.646	96.802	0.442
comGB	Lactococcus lactis subsp. cremoris KW2	42.769	94.477	0.404