Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   V1M48_RS11465 Genome accession   NZ_CP143559
Coordinates   334842..335858 (+) Length   338 a.a.
NCBI ID   WP_029789968.1    Uniprot ID   -
Organism   Vibrio harveyi strain WHSS0915     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329842..340858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1M48_RS11450 (V1M48_11450) - 330215..330943 (-) 729 WP_005451494.1 redoxin family protein -
  V1M48_RS11455 (V1M48_11455) oxyR 331118..332026 (+) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  V1M48_RS11460 (V1M48_11460) - 332093..334663 (-) 2571 WP_199479218.1 penicillin-binding protein 1A -
  V1M48_RS11465 (V1M48_11465) pilM 334842..335858 (+) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  V1M48_RS11470 (V1M48_11470) pilN 335842..336417 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  V1M48_RS11475 (V1M48_11475) pilO 336410..337003 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  V1M48_RS11480 (V1M48_11480) pilP 336993..337508 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  V1M48_RS11485 (V1M48_11485) pilQ 337543..339294 (+) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  V1M48_RS11490 (V1M48_11490) aroK 339484..340002 (+) 519 WP_005451507.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38023.40 Da        Isoelectric Point: 6.4206

>NTDB_id=929668 V1M48_RS11465 WP_029789968.1 334842..335858(+) (pilM) [Vibrio harveyi strain WHSS0915]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=929668 V1M48_RS11465 WP_029789968.1 334842..335858(+) (pilM) [Vibrio harveyi strain WHSS0915]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494