Detailed information NTG0000672

Experimentally validated

Overview

• Name: radC
• Type: Machinery gene
• Locus tag: SGO_RS06035
• Genome accession: NC_009785
• Coordinates: 1274670..1275350 (-)
• Length: 226 a.a.
• NCBI ID: WP_012000629.1
• Uniprot ID: A8AXK6
• Organism: Streptococcus gordonii str. Challis substr. CH1
• Function: homologous recombination Homologous recombination

Function

radC is expressed specifically in cells competent for genetic transformation

Genomic Context

Location: 1269670..1280350

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGO_RS06000 (SGO_1222)	-	1269792..1270457 (-)	666	WP_012000623.1	GTP pyrophosphokinase	-
SGO_RS06005 (SGO_1223)	-	1270560..1271129 (+)	570	WP_012000624.1	CYTH domain-containing protein	-
SGO_RS06010 (SGO_1224)	-	1271201..1272157 (+)	957	WP_111724295.1	ribose-phosphate diphosphokinase	-
SGO_RS06015 (SGO_1225)	-	1272167..1273294 (+)	1128	WP_012000626.1	cysteine desulfurase family protein	-
SGO_RS06020 (SGO_1226)	-	1273284..1273631 (+)	348	WP_008809173.1	DUF1831 domain-containing protein	-
SGO_RS06025 (SGO_1227)	-	1273666..1273899 (+)	234	WP_012000627.1	DUF4649 family protein	-
SGO_RS06030 (SGO_1228)	-	1273985..1274650 (+)	666	WP_041789693.1	redox-sensing transcriptional repressor Rex	-
SGO_RS06035 (SGO_1229)	radC	1274670..1275350 (-)	681	WP_012000629.1	RadC family protein	Machinery gene
SGO_RS06040 (SGO_1230)	-	1275432..1276187 (-)	756	WP_012000630.1	class A sortase	-
SGO_RS06045 (SGO_1231)	gyrA	1276191..1278644 (-)	2454	WP_012000631.1	DNA gyrase subunit A	-
SGO_RS06050 (SGO_1232)	-	1278842..1279828 (+)	987	WP_012000632.1	L-lactate dehydrogenase	-
SGO_RS11060	-	1279825..1280058 (-)	234	Protein_1200	hypothetical protein	-

Sequence

Protein

• Length: 226 a.a.
• Molecular weight: 25617.43 Da
• Isoelectric Point: 6.7740

>NTDB_id=672 SGO_RS06035 WP_012000629.1 1274670..1275350(-) (radC) [Streptococcus gordonii str. Challis substr. CH1]
MYSISFQEESLLPRERLVRLGAEQLSNQELLSILIRTGNKKENVFQIASKILSSVSSLTELRHMTLSELQGMSGIGQVKA
IELQAMIELGSRISKAENFEGERILSSQKLAKKMQQELSHKKQEHLVALYLNTQNQIIHQQTIFIGSLTRSLAEPREILH
YAIKHMATSIILVHNHPSGATRPSRDDDQVTEKIKEACEMMGFVLLDHLIVGGDSYYSYREETDLI

Nucleotide

• Length: 681 bp

>NTDB_id=672 SGO_RS06035 WP_012000629.1 1274670..1275350(-) (radC) [Streptococcus gordonii str. Challis substr. CH1]
ATGTACAGTATATCATTTCAAGAGGAATCATTATTACCACGGGAACGATTAGTAAGACTAGGAGCAGAGCAGTTAAGCAA
TCAGGAATTGCTTTCCATTCTCATTCGTACAGGAAATAAAAAAGAGAATGTATTCCAAATAGCTAGTAAGATTTTATCAT
CCGTATCAAGTTTGACTGAACTCCGTCACATGACTCTGTCTGAACTACAGGGGATGTCTGGTATAGGGCAAGTAAAAGCG
ATTGAGCTACAGGCCATGATTGAGTTGGGTAGTCGTATTTCAAAAGCGGAAAATTTTGAAGGAGAACGTATACTGAGTAG
TCAAAAACTTGCTAAGAAAATGCAACAGGAATTAAGTCATAAAAAGCAAGAGCATTTGGTTGCACTTTATTTGAACACAC
AAAATCAGATTATTCATCAACAAACTATTTTCATTGGAAGTCTGACTCGGAGTTTAGCCGAACCTAGAGAGATTCTTCAT
TATGCGATTAAGCATATGGCGACATCAATTATCTTGGTCCATAATCATCCTTCAGGCGCAACTAGGCCAAGTAGGGACGA
TGATCAGGTGACAGAGAAAATAAAAGAAGCCTGCGAAATGATGGGCTTCGTTTTATTGGATCATTTAATTGTGGGAGGGG
ATAGCTATTATAGTTATCGAGAGGAAACAGACCTCATTTAA

Domains

Predicted by InterProScan.

• RadC-like JAB domain (RadC) (106-222)

• UPF0758 N-terminal (UPF0758_N) (11-81)

3D structure

• AlphaFold DB: A8AXK6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radC	Streptococcus pneumoniae TIGR4	76.106	100	0.761
radC	Streptococcus pneumoniae D39	75.664	100	0.757
radC	Streptococcus pneumoniae R6	75.664	100	0.757
ysxA/radC	Bacillus subtilis subsp. subtilis str. 168	46.667	92.92	0.434

References

• [1]: M M Vickerman et al. (2007) Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide. Journal of Bacteriology 189(21):7799-807. [PMID: 17720781]