Detailed information    

experimental Experimentally validated

Overview

Name   radC   Type   Machinery gene
Locus tag   SPD_RS05240 Genome accession   NC_008533
Coordinates   989008..989688 (+) Length   226 a.a.
NCBI ID   WP_000286923.1    Uniprot ID   Q8DPU7
Organism   Streptococcus pneumoniae D39     
Function   homologous recombination   
Homologous recombination

Function

radC is expressed specifically in cells competent for genetic transformation


Genomic Context

Location: 984008..994688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS05220 (SPD_0970) - 984440..985300 (+) 861 WP_000631543.1 methionyl aminopeptidase -
  SPD_RS05230 - 985681..986483 (+) 803 WP_106269370.1 IS5 family transposase -
  SPD_RS05235 (SPD_0973) pcrA 986674..988965 (+) 2292 WP_000992853.1 DNA helicase PcrA -
  SPD_RS05240 (SPD_0975) radC 989008..989688 (+) 681 WP_000286923.1 RadC family protein Machinery gene
  SPD_RS05245 (SPD_0974) - 989685..990374 (-) 690 WP_000743331.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  SPD_RS05250 (SPD_0976) - 990392..991033 (-) 642 WP_000653405.1 redox-sensing transcriptional repressor Rex -
  SPD_RS05255 (SPD_0977) - 991116..991331 (-) 216 WP_000462488.1 DUF4649 family protein -
  SPD_RS05260 (SPD_0978) - 991339..991686 (-) 348 WP_000863509.1 DUF1831 domain-containing protein -
  SPD_RS05265 (SPD_0979) - 991691..992806 (-) 1116 WP_000639657.1 cysteine desulfurase family protein -
  SPD_RS05270 (SPD_0980) - 992816..993775 (-) 960 WP_001283813.1 ribose-phosphate diphosphokinase -
  SPD_RS05275 (SPD_0981) - 993888..994457 (-) 570 WP_000681292.1 CYTH domain-containing protein -

Sequence

Protein


Download         Length: 226 a.a.        Molecular weight: 25521.51 Da        Isoelectric Point: 7.1203

>NTDB_id=668 SPD_RS05240 WP_000286923.1 989008..989688(+) (radC) [Streptococcus pneumoniae D39]
MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASVFEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKA
IELQAMIELGHRIHKHETLEMESILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
YAIKHMATSLVLVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFSYREKTDLI

Nucleotide


Download         Length: 681 bp        

>NTDB_id=668 SPD_RS05240 WP_000286923.1 989008..989688(+) (radC) [Streptococcus pneumoniae D39]
ATGTACAGTATTTCATTCCAAGAAGATTCACTATTACCAAGAGAAAGGCTGGCCAAGGAAGGAGTTGAAGCGCTTAGTAA
CCAAGAGTTGCTAGCTATTTTACTCAGGACAGGAACACGTCAAGCTAGCGTTTTTGAAATTGCCCAAAAAGTCTTGAACA
ATCTTTCAAGCCTAACGGATTTGAAAAAAATGACCCTGCAGGAATTGCAGAGTTTGTCTGGTATTGGGCGTGTTAAGGCC
ATAGAATTACAAGCTATGATTGAACTGGGGCATCGTATTCACAAACACGAGACTCTTGAAATGGAAAGTATTCTCAGCAG
TCAAAAGTTGGCCAAGAAGATGCAGCAGGAATTAGGGGATAAAAAACAAGAGCACCTGGTGGCACTCTATCTCAATACTC
AAAATCAAATCATCCATCAGCAGACCATTTTTATCGGGTCTGTAACTCGTAGTATCGCTGAACCGCGAGAGATTCTTCAC
TATGCAATCAAGCATATGGCGACTTCTCTTGTCTTGGTCCACAATCATCCTTCAGGAGCGGTAGCGCCTAGCCAAAATGA
TGATCATGTCACTAAACTTGTTAAAGAAGCCTGCGAACTGATGGGGATTGTTCTCTTGGACCATTTGATTGTCTCTCATT
CTAATTACTTTAGTTATCGTGAAAAGACAGATTTAATCTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(13-81)

RadC-like JAB domain (RadC)

(106-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DPU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Streptococcus pneumoniae R6

100

100

1
  radC Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radC Streptococcus gordonii str. Challis substr. CH1

75.664

100

0.757
  ysxA/radC Bacillus subtilis subsp. subtilis str. 168

47.619

92.92

0.442

References

[1] J A Ruiz-Masó et al. (2006) Genetic and biochemical characterization of the Streptococcus pneumoniae PcrA helicase and its role in plasmid rolling circle replication. Journal of Bacteriology 188(21):7416-25. [PMID: 16936036]
[2] Jae-Hoon Song et al. (2005) Identification of essential genes in Streptococcus pneumoniae by allelic replacement mutagenesis. Molecules And Cells 19(3):365-74. [PMID: 15995353]