Detailed information    

experimental Experimentally validated

Overview

Name   radC   Type   Machinery gene
Locus tag   SP_RS05390 Genome accession   NC_003028
Coordinates   1024899..1025579 (+) Length   226 a.a.
NCBI ID   WP_000286922.1    Uniprot ID   Q97QW0
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

radC is expressed specifically in cells competent for genetic transformation


Genomic Context

Location: 1019899..1030579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS05360 (SP_1084) - 1020331..1021191 (+) 861 WP_000631543.1 methionyl aminopeptidase -
  SP_RS13780 - 1021416..1021574 (+) 159 WP_001827319.1 hypothetical protein -
  SP_RS12110 - 1021571..1022374 (+) 804 Protein_1026 IS5 family transposase -
  SP_RS05385 (SP_1087) pcrA 1022565..1024856 (+) 2292 WP_000992862.1 DNA helicase PcrA -
  SP_RS05390 (SP_1088) radC 1024899..1025579 (+) 681 WP_000286922.1 RadC family protein Machinery gene
  SP_RS05395 (SP_1089) - 1025576..1026265 (-) 690 WP_000743330.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  SP_RS05400 (SP_1090) - 1026283..1026924 (-) 642 WP_000653403.1 redox-sensing transcriptional repressor Rex -
  SP_RS05405 (SP_1092) - 1027007..1027222 (-) 216 WP_000462487.1 DUF4649 family protein -
  SP_RS05410 (SP_1093) - 1027230..1027577 (-) 348 WP_000863509.1 DUF1831 domain-containing protein -
  SP_RS05415 (SP_1094) - 1027582..1028697 (-) 1116 WP_000639669.1 cysteine desulfurase family protein -
  SP_RS05420 (SP_1095) - 1028707..1029666 (-) 960 WP_001283813.1 ribose-phosphate diphosphokinase -
  SP_RS05425 (SP_1096) - 1029779..1030348 (-) 570 WP_000681292.1 CYTH domain-containing protein -

Sequence

Protein


Download         Length: 226 a.a.        Molecular weight: 25535.53 Da        Isoelectric Point: 7.1203

>NTDB_id=670 SP_RS05390 WP_000286922.1 1024899..1025579(+) (radC) [Streptococcus pneumoniae TIGR4]
MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASVFEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKA
IELQAMIELGHRIHKHETLEMESILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFSYREKTDLI

Nucleotide


Download         Length: 681 bp        

>NTDB_id=670 SP_RS05390 WP_000286922.1 1024899..1025579(+) (radC) [Streptococcus pneumoniae TIGR4]
ATGTACAGTATTTCATTCCAAGAAGATTCACTATTACCAAGAGAAAGGCTGGCCAAGGAAGGAGTTGAAGCGCTTAGTAA
CCAAGAGTTGCTAGCTATTTTACTCAGGACAGGAACACGTCAAGCTAGCGTTTTTGAAATTGCCCAAAAAGTCTTGAACA
ATCTTTCAAGCCTAACGGATTTGAAAAAAATGACCCTGCAGGAATTGCAGAGTTTGTCTGGTATTGGGCGTGTTAAGGCC
ATAGAATTACAAGCTATGATTGAACTGGGGCATCGTATTCACAAACACGAGACTCTTGAAATGGAAAGTATTCTCAGCAG
TCAAAAGTTGGCCAAGAAGATGCAGCAGGAATTAGGGGATAAAAAACAAGAGCACCTGGTGGCACTCTATCTCAATACTC
AAAATCAAATCATCCATCAGCAGACCATTTTTATCGGGTCTGTAACTCGTAGTATCGCTGAACCGCGAGAGATTCTTCAC
TATGCAATCAAGCATATGGCGACTTCTCTTATCTTGGTCCACAATCATCCTTCAGGAGCGGTAGCGCCTAGCCAAAATGA
TGATCATGTCACTAAACTTGTTAAAGAAGCCTGCGAATTGATGGGGATTGTTCTCTTGGACCATTTGATTGTCTCTCATT
CTAATTACTTTAGTTATCGTGAAAAGACAGATTTAATCTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(13-81)

RadC-like JAB domain (RadC)

(106-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q97QW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Streptococcus pneumoniae D39

99.558

100

0.996
  radC Streptococcus pneumoniae R6

99.558

100

0.996
  radC Streptococcus gordonii str. Challis substr. CH1

76.106

100

0.761
  ysxA/radC Bacillus subtilis subsp. subtilis str. 168

48.095

92.92

0.447

References

[1] J A Ruiz-Masó et al. (2006) Genetic and biochemical characterization of the Streptococcus pneumoniae PcrA helicase and its role in plasmid rolling circle replication. Journal of Bacteriology 188(21):7416-25. [PMID: 16936036]
[2] Jae-Hoon Song et al. (2005) Identification of essential genes in Streptococcus pneumoniae by allelic replacement mutagenesis. Molecules And Cells 19(3):365-74. [PMID: 15995353]