Detailed information    

experimental Experimentally validated

Overview

Name   ysxA/radC   Type   Machinery gene
Locus tag   BSU_28040 Genome accession   NC_000964
Coordinates   2861840..2862535 (-) Length   231 a.a.
NCBI ID   NP_390682.1    Uniprot ID   Q02170
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

radC is expressed specifically in cells competent for genetic transformation


Genomic Context

Location: 2856840..2867535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_27990 (BSU27990) minD 2857776..2858582 (-) 807 NP_390677.1 ATPase activator of MinC -
  BSU_28000 (BSU28000) minC 2858584..2859264 (-) 681 NP_390678.1 cell-division regulator (septum placement) -
  BSU_28010 (BSU28010) mreD 2859317..2859835 (-) 519 NP_390679.1 cell-shape determining protein -
  BSU_28020 (BSU28020) mreC 2859832..2860704 (-) 873 NP_390680.2 cell-shape determining protein -
  BSU_28030 (BSU28030) mreB 2860735..2861748 (-) 1014 NP_390681.2 cell-shape determining protein -
  BSU_28040 (BSU28040) ysxA/radC 2861840..2862535 (-) 696 NP_390682.1 conserved nucleotide-related metabolism protein Machinery gene
  BSU_28050 (BSU28050) maf 2862572..2863141 (-) 570 NP_390683.1 nucleoside triphosphate pyrophosphatase; septum formation DNA-binding protein (multicopy associated filamentation) -
  BSU_28060 (BSU28060) spoIIB 2863294..2864292 (-) 999 NP_390684.1 spatial and temporal regulator of the dissolution of septal peptidoglycan during engulfment (stage II sporulation) -
  BSU_28070 (BSU28070) comC 2864426..2865172 (-) 747 NP_390685.2 membrane prepilin peptidase Machinery gene
  BSU_28080 (BSU28080) folC 2865312..2866604 (-) 1293 NP_390686.1 folyl-polyglutamate synthase -

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 26146.48 Da        Isoelectric Point: 7.9285

>NTDB_id=671 BSU_28040 NP_390682.1 2861840..2862535(-) (ysxA/radC) [Bacillus subtilis subsp. subtilis str. 168]
MVIHDLPLKLKDFPMKEKPRERLLKVGAENLANHELLAILLRTGTKHESVLDLSNRLLRSFDGLRLLKEASVEELSSIPG
IGMVKAIQILAAVELGSRIHKLANEEHFVIRSPEDGANLVMEDMRFLTQEHFVCLYLNTKNQVIHKRTVFIGSLNSSIVH
PREVFKEAFKRSAASFICVHNHPSGDPTPSREDIEVTRRLFECGNLIGIELLDHLVIGDKKFVSLKEKGYL

Nucleotide


Download         Length: 696 bp        

>NTDB_id=671 BSU_28040 NP_390682.1 2861840..2862535(-) (ysxA/radC) [Bacillus subtilis subsp. subtilis str. 168]
TTGGTCATACACGATCTGCCATTAAAACTCAAAGATTTCCCAATGAAAGAAAAGCCAAGGGAACGGCTCCTGAAAGTCGG
AGCCGAGAACTTAGCGAATCATGAACTTTTGGCTATATTATTGCGGACAGGGACTAAACACGAATCTGTTTTGGACCTGT
CAAACCGGCTGCTGCGCTCATTTGACGGGCTGCGCCTGCTCAAGGAAGCATCGGTTGAAGAGCTGTCAAGCATCCCGGGA
ATCGGTATGGTAAAAGCGATTCAAATACTGGCAGCAGTTGAGCTTGGAAGCCGGATTCATAAATTAGCCAACGAAGAACA
TTTCGTTATTCGCTCCCCGGAAGACGGCGCTAATCTTGTCATGGAGGATATGCGCTTTTTAACCCAGGAGCATTTTGTCT
GTTTATACTTAAATACAAAAAATCAAGTCATCCATAAACGCACCGTATTTATCGGAAGCCTGAATTCATCTATTGTCCAC
CCGCGAGAGGTGTTTAAAGAAGCGTTTAAACGATCTGCCGCTTCCTTTATCTGTGTTCATAATCATCCTTCTGGAGATCC
GACGCCGAGCAGGGAAGATATTGAAGTGACAAGACGGCTGTTTGAATGCGGAAACCTGATTGGCATCGAGCTGCTTGACC
ATTTGGTGATCGGGGATAAAAAATTTGTGAGTTTAAAGGAAAAAGGATATTTGTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(110-229)

UPF0758 N-terminal (UPF0758_N)

(11-87)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q02170

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Streptococcus pneumoniae TIGR4

48.095

92.92

0.447
  radC Streptococcus pneumoniae D39

47.619

92.92

0.442
  radC Streptococcus pneumoniae R6

47.619

92.92

0.442
  radC Streptococcus gordonii str. Challis substr. CH1

46.667

92.92

0.434

References

[1] Randy M Berka et al. (2002) Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK. Molecular Microbiology 43(5):1331-45. [PMID: 11918817]
[2] Mitsuo Ogura et al. (2002) Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK. Journal of Bacteriology 184(9):2344-51. [PMID: 11948146]
[3] Leendert W Hamoen et al. (2002) Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach. Nucleic Acids Research 30(24):5517-28. [PMID: 12490720]