Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   VP459_RS13475 Genome accession   NZ_CP142600
Coordinates   2750319..2750816 (+) Length   165 a.a.
NCBI ID   WP_029697681.1    Uniprot ID   A0AAE2ZZ83
Organism   Staphylococcus aureus strain M19060305     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2745319..2755816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP459_RS13435 (VP459_13440) - 2745363..2746349 (+) 987 WP_000161315.1 ROK family glucokinase -
  VP459_RS13440 (VP459_13445) - 2746349..2746678 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  VP459_RS13445 (VP459_13450) - 2746675..2747298 (+) 624 WP_001223006.1 MBL fold metallo-hydrolase -
  VP459_RS13450 (VP459_13455) comGA 2747350..2748324 (+) 975 WP_000697215.1 competence type IV pilus ATPase ComGA Machinery gene
  VP459_RS13455 (VP459_13460) comGB 2748296..2749366 (+) 1071 WP_000776403.1 competence type IV pilus assembly protein ComGB Machinery gene
  VP459_RS13460 (VP459_13465) comGC 2749380..2749691 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  VP459_RS13465 (VP459_13470) comGD 2749669..2750115 (+) 447 WP_075583651.1 competence type IV pilus minor pilin ComGD Machinery gene
  VP459_RS13470 (VP459_13475) comGE 2750102..2750401 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  VP459_RS13475 (VP459_13480) comGF 2750319..2750816 (+) 498 WP_029697681.1 competence type IV pilus minor pilin ComGF Machinery gene
  VP459_RS13480 (VP459_13485) - 2750913..2751059 (+) 147 WP_001789879.1 hypothetical protein -
  VP459_RS13485 (VP459_13490) - 2751049..2751573 (+) 525 WP_001015122.1 shikimate kinase -
  VP459_RS13490 (VP459_13495) gcvT 2751732..2752823 (+) 1092 WP_000093355.1 glycine cleavage system aminomethyltransferase GcvT -
  VP459_RS13495 (VP459_13500) gcvPA 2752843..2754189 (+) 1347 WP_000019688.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  VP459_RS13500 (VP459_13505) gcvPB 2754182..2755654 (+) 1473 WP_000202191.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19211.94 Da        Isoelectric Point: 10.2436

>NTDB_id=926167 VP459_RS13475 WP_029697681.1 2750319..2750816(+) (comGF) [Staphylococcus aureus strain M19060305]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFRGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=926167 VP459_RS13475 WP_029697681.1 2750319..2750816(+) (comGF) [Staphylococcus aureus strain M19060305]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAGAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(69-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976