Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   VKP34_RS00655 Genome accession   NZ_CP142358
Coordinates   103002..104036 (+) Length   344 a.a.
NCBI ID   WP_247593334.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 6702-99     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98002..109036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKP34_RS00645 (VKP34_00645) - 101670..102035 (+) 366 WP_002986560.1 DUF1033 family protein -
  VKP34_RS00650 (VKP34_00650) comYA 102128..103066 (+) 939 WP_023612548.1 competence type IV pilus ATPase ComGA Machinery gene
  VKP34_RS00655 (VKP34_00655) comYB 103002..104036 (+) 1035 WP_247593334.1 competence type IV pilus assembly protein ComGB Machinery gene
  VKP34_RS00660 (VKP34_00660) comYC 104038..104364 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  VKP34_RS00665 (VKP34_00665) comGD 104339..104767 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  VKP34_RS00670 (VKP34_00670) comGE 104724..105008 (+) 285 Protein_85 competence type IV pilus minor pilin ComGE -
  VKP34_RS00675 (VKP34_00675) comYF 105001..105435 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  VKP34_RS00680 (VKP34_00680) comGG 105419..105745 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  VKP34_RS00685 (VKP34_00685) comYH 105843..106796 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  VKP34_RS00690 (VKP34_00690) - 106855..108051 (+) 1197 WP_109821056.1 acetate kinase -
  VKP34_RS00695 (VKP34_00695) - 108238..108546 (+) 309 WP_032467031.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39718.23 Da        Isoelectric Point: 9.7085

>NTDB_id=925407 VKP34_RS00655 WP_247593334.1 103002..104036(+) (comYB) [Streptococcus pyogenes strain 6702-99]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIVFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=925407 VKP34_RS00655 WP_247593334.1 103002..104036(+) (comYB) [Streptococcus pyogenes strain 6702-99]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGTTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404