Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VKP34_RS00675 Genome accession   NZ_CP142358
Coordinates   105001..105435 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 6702-99     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100001..110435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKP34_RS00645 (VKP34_00645) - 101670..102035 (+) 366 WP_002986560.1 DUF1033 family protein -
  VKP34_RS00650 (VKP34_00650) comYA 102128..103066 (+) 939 WP_023612548.1 competence type IV pilus ATPase ComGA Machinery gene
  VKP34_RS00655 (VKP34_00655) comYB 103002..104036 (+) 1035 WP_247593334.1 competence type IV pilus assembly protein ComGB Machinery gene
  VKP34_RS00660 (VKP34_00660) comYC 104038..104364 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  VKP34_RS00665 (VKP34_00665) comGD 104339..104767 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  VKP34_RS00670 (VKP34_00670) comGE 104724..105008 (+) 285 Protein_85 competence type IV pilus minor pilin ComGE -
  VKP34_RS00675 (VKP34_00675) comYF 105001..105435 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  VKP34_RS00680 (VKP34_00680) comGG 105419..105745 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  VKP34_RS00685 (VKP34_00685) comYH 105843..106796 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  VKP34_RS00690 (VKP34_00690) - 106855..108051 (+) 1197 WP_109821056.1 acetate kinase -
  VKP34_RS00695 (VKP34_00695) - 108238..108546 (+) 309 WP_032467031.1 hypothetical protein -
  VKP34_RS00700 (VKP34_00700) proC 108629..109399 (-) 771 WP_002992745.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=925409 VKP34_RS00675 WP_002992738.1 105001..105435(+) (comYF) [Streptococcus pyogenes strain 6702-99]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=925409 VKP34_RS00675 WP_002992738.1 105001..105435(+) (comYF) [Streptococcus pyogenes strain 6702-99]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396